An in vivo brain-distribution study was carried out using Wistar albino rats divided into two groups of six animals each. For the first group, a drug suspension was administered intranasally by holding the rats in a slanted position. For the second group, a plain drug suspension was administered intravenously via tuberculin syringe. The rats were euthanized using CO2 at different time intervals after blood collection via retro-orbital puncture. Their brains were then excised for analysis.

1. In-vivo Brain-distribution study in rats
Mr. Sagar Kishor Savale
M. Pharm (Pharmaceutics)

2. In vivo pharmacokinetic study was carried out using Wistar albino rats
Mr. Sagar Kishor Savale

3. This in vivo brain-distribution studies can be divided in two groups each group
consist of six animals.
Groups I Groups II
Mr. Sagar Kishor Savale

4. The rats are held from the back in slanted
position during intranasal (IN) administration
Groups I
Mr. Sagar Kishor Savale

5. Groups II
For second group plain drug suspension (PDS) are administered in intravenously (IV) with the help of
tuberculin syringe (1 mL), at the delivery site.
Mr. Sagar Kishor Savale

6. Mr. Sagar Kishor Savale

7. The rats are euthanatized by using CO2 chamber
Mr. Sagar Kishor Savale

8. The animals were decapitated immediately
after blood collection by retro-orbital plexus
puncture (at different time of interval)
Mr. Sagar Kishor Savale

9. The rats are sacrificed at different time of intervals
Mr. Sagar Kishor Savale

10. After Blood Collection, Skull was openand the brain was carefully excised
Mr. Sagar Kishor Savale

11. Mr. Sagar Kishor Savale

12. Contact
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