I\'m having issues with two homework questions. I get the gist of the mechanisms involved with transcription and translation, but the question itself is really throwing me off. 4. What kinds of mutations might result in transcriptional errors? What kinds of mutations might result in translational errors? What I\'m thinking for this one is that transcriptional errors are having to do with radiation-type mutations as well as incidences where the RNA polymerase or other polymerases make a mistake when creating that RNA template and the mistake does not get fixed. As far as the translational side, I\'m kind of clueless. Part of this homework is in the realm of CFTR and the delF508 gene and the W1282X gene, as well as nonsense and missense mutations. I do not know where they fall into this stuff. 6. Which part of the central dogma is represented by CFTRdel508 and by W1282X? Where is the information flow interrupted (transcription or translation)? Justify your answer. Again, I get the gist, but not really how these actually fit into the question. I BELIEVE the answer to be that the problem / informational flow interruption occurs during translation, but I\'m not sure that\'s correct, nor can I justify it. THANKS FOR THE HELP! :) Solution 4. Mutation like point mutations, framshift mutations, chromosomal disjunction etc causes incorrect base pairing at the DNA level, hence the RNA polymerase incorporates incorrect nucleotides and thereby creating incorrect mRNA sequences. Also, any mutations in RNA polymerase, and other transcriptional factors lead to transxccriptional errors Similarly the mRNA is incorrect then the ribosome and the tRNA also incorporates incorrect amino acids thereby forming a defective protein which leads to translational errors. Also, any mutation of the tRNA/ ribosome\'s sub units (50 S and 30 S) can also lead to translational errors thereby leading to a defective protein. 6. DNA ----> RNA ----> Protein The deletions inthe 2 genes fall in the DNA to RNA part of central dogma. Any mutations of the CFTRdel508 and W1282X gene causes the protein folding to be disrupted. The protein in question for these 2 genes is Cystic Fibrosis transmembrane conductance regulator (CFTR). Since the protein is unable to fold properly (the missense mutation in CFTRdel508 causing the Phenylalanine not to get coded) hence the protein is unable to move out of the Endoplasmic Reticulum. Hence, the probelm is not at the translation level but mainly at the transcription level, since the deletion had occured in the DNA which resulted in the coding of an incorrect mRNA thereby forming a defective protein. So the major problem is at the transcriptional level..

1. I'm having issues with two homework questions. I get the gist of the mechanisms involved with
transcription and translation, but the question itself is really throwing me off.
4. What kinds of mutations might result in transcriptional errors? What kinds of mutations might
result in translational errors?
What I'm thinking for this one is that transcriptional errors are having to do with radiation-type
mutations as well as incidences where the RNA polymerase or other polymerases make a
mistake when creating that RNA template and the mistake does not get fixed. As far as the
translational side, I'm kind of clueless. Part of this homework is in the realm of CFTR and the
delF508 gene and the W1282X gene, as well as nonsense and missense mutations. I do not know
where they fall into this stuff.
6. Which part of the central dogma is represented by CFTRdel508 and by W1282X? Where is
the information flow interrupted (transcription or translation)? Justify your answer.
Again, I get the gist, but not really how these actually fit into the question. I BELIEVE the
answer to be that the problem / informational flow interruption occurs during translation, but I'm
not sure that's correct, nor can I justify it. THANKS FOR THE HELP! :)
Solution
4. Mutation like point mutations, framshift mutations, chromosomal disjunction etc causes
incorrect base pairing at the DNA level, hence the RNA polymerase incorporates incorrect
nucleotides and thereby creating incorrect mRNA sequences. Also, any mutations in RNA
polymerase, and other transcriptional factors lead to transxccriptional errors
Similarly the mRNA is incorrect then the ribosome and the tRNA also incorporates incorrect
amino acids thereby forming a defective protein which leads to translational errors. Also, any
mutation of the tRNA/ ribosome's sub units (50 S and 30 S) can also lead to translational errors
thereby leading to a defective protein.
6. DNA ----> RNA ----> Protein
The deletions inthe 2 genes fall in the DNA to RNA part of central dogma.
Any mutations of the CFTRdel508 and W1282X gene causes the protein folding to be disrupted.
The protein in question for these 2 genes is Cystic Fibrosis transmembrane conductance
regulator (CFTR). Since the protein is unable to fold properly (the missense mutation in
CFTRdel508 causing the Phenylalanine not to get coded) hence the protein is unable to move out
of the Endoplasmic Reticulum.
Hence, the probelm is not at the translation level but mainly at the transcription level, since the
deletion had occured in the DNA which resulted in the coding of an incorrect mRNA thereby

2. forming a defective protein.
So the major problem is at the transcriptional level.