Why aren\'t all detectors suitable for gradient elution methods in HPLC? You should include an example of a detection system that works with it, and one that does not, but also give reason for their success/failure as a gradient elution detector.
Solution
The gradient elution methods used for heavy molecular weight component move faster in mobile phase of HPLC/Chromatography, and least molecular weight component move slower in elucidation of chromatography.
The detection in HPLC due to absorbance, In HPLC UV detector and refractive index detector are commenly used.
UV detector is easy to operate and provide good stability compared to all other detector.
More preferebly. differential refractometer (DRI), or refractive index detector (RI or RID) is measures the refractive index of an analyte relative to the solvent.
.
A brief review on development and validation of hplc method.adhirajain
the slides in the ppt gives a brief review on product development and its validation in HPLC method. Contents are with advantages, disadvantages, application , classification and methods for development.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
A brief review on development and validation of hplc method.adhirajain
the slides in the ppt gives a brief review on product development and its validation in HPLC method. Contents are with advantages, disadvantages, application , classification and methods for development.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
HPLC BMLT/ DMLT High Performance Liquid ChromatographyMdShamsTabrez4
Chromatography is a separation technique used to separate and analyze the components of a mixture. It involves the distribution of the components between two phases: a mobile phase and a stationary phase. The different affinities of the components for these phases lead to their separation based on their physical or chemical properties.
This presentation was presented when I was MVSc 1st year student, hence this ppt will provide a piece of basic information about the use of Chromatography.in toxicology. Principle of its work and related terminology are explained in this ppt.
Wilson Corporation is budgeting its equipment needs on an ongoing basi.docxrichard981
Wilson Corporation is budgeting its equipment needs on an ongoing basis, with a new quarter being added to the budget as the current quarter is completed. This type of budget is most commonly known as a:
capital budget.
rolling budget.
revised budget.
pro-forma budget.
financial budget.
Solution
Wilson Corporation is budgeting its equipment needs on an ongoing basis, with a new quarter being added to the budget as the current quarter is completed. This type of budget is most commonly known as a:
Rolling Budget
Note : This type of Budget is also known as continuos Budget where Rolling or Continuos process is being performed after the end of process and repeatedly performed . A rolling budget is continually updated to add a new budget period as the most recent budget period is completed. Thus, the rolling budget involves the incremental extension of the existing budget model
.
Windows hides most of its configuration in a binary file called a regi.docxrichard981
Windows hides most of its configuration in a binary file called a registry. Special Windows tools must be used to read and modify the registry. What are the advantages and disadvantages of this approach as opposed to using text-based configuration files that other operating systems favor? Identify one configuration item that you could change in the registry.
Solution
There are so many advantages provided by the design of window registry over text based.INI files (INI is file extension for initialization file format used by Microsoft windows) used in predecessors of windows95 operating system.
The disadvantages of the window registry are:
There are so many advantages provided by the design of window registry over text based.INI files (INI is file extension for initialization file format used by Microsoft windows) used in predecessors of windows95 operating system.
The disadvantages of the window registry are:
.
More Related Content
Similar to Why aren-'t all detectors suitable for gradient elution methods in HPL.docx
HPLC stands for “High-performance liquid chromatography”(sometimes referred to as High-pressure liquid chromatography).
High performance liquid chromatography is a powerful tool in analysis, it yields high performance and high speed compared to traditional columns chromatography because of the forcibly pumped mobile phase.
It is used in biochemistry and analytical chemistry to identify, quantify and purify the individual components of a mixture.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
HPLC BMLT/ DMLT High Performance Liquid ChromatographyMdShamsTabrez4
Chromatography is a separation technique used to separate and analyze the components of a mixture. It involves the distribution of the components between two phases: a mobile phase and a stationary phase. The different affinities of the components for these phases lead to their separation based on their physical or chemical properties.
This presentation was presented when I was MVSc 1st year student, hence this ppt will provide a piece of basic information about the use of Chromatography.in toxicology. Principle of its work and related terminology are explained in this ppt.
Similar to Why aren-'t all detectors suitable for gradient elution methods in HPL.docx (20)
Wilson Corporation is budgeting its equipment needs on an ongoing basi.docxrichard981
Wilson Corporation is budgeting its equipment needs on an ongoing basis, with a new quarter being added to the budget as the current quarter is completed. This type of budget is most commonly known as a:
capital budget.
rolling budget.
revised budget.
pro-forma budget.
financial budget.
Solution
Wilson Corporation is budgeting its equipment needs on an ongoing basis, with a new quarter being added to the budget as the current quarter is completed. This type of budget is most commonly known as a:
Rolling Budget
Note : This type of Budget is also known as continuos Budget where Rolling or Continuos process is being performed after the end of process and repeatedly performed . A rolling budget is continually updated to add a new budget period as the most recent budget period is completed. Thus, the rolling budget involves the incremental extension of the existing budget model
.
Windows hides most of its configuration in a binary file called a regi.docxrichard981
Windows hides most of its configuration in a binary file called a registry. Special Windows tools must be used to read and modify the registry. What are the advantages and disadvantages of this approach as opposed to using text-based configuration files that other operating systems favor? Identify one configuration item that you could change in the registry.
Solution
There are so many advantages provided by the design of window registry over text based.INI files (INI is file extension for initialization file format used by Microsoft windows) used in predecessors of windows95 operating system.
The disadvantages of the window registry are:
There are so many advantages provided by the design of window registry over text based.INI files (INI is file extension for initialization file format used by Microsoft windows) used in predecessors of windows95 operating system.
The disadvantages of the window registry are:
.
Wile E- Coyote is dropping from cliff- but having been in this sort of.docxrichard981
Wile E. Coyote is dropping from cliff, but having been in this sort of situation before, he planned ahead. He is carrving an anvil that is ten times his own weight and plans to to throw the anvil downwards as he falls. He has calculated that he throws it hard enough, he will reduce his impact speed by half of what it would have been (if he just fell freely he\'d hit the ground at a speed of 24 m/s). How fast is the anvil going to be moving when it hits the ground, if all goes according to plan?
Solution
conserving momentum before and after throwing the anvil:
mass of the person*speed of the person when it hits ground without the anvil=mass of the person*impat speed after anvil is hit +mass of the anvil*impact speed for the anvil
==>m*24=m*(24/2)+10*m*impact speed of anvil
==>12*m=10*m*impact speed of anvil
==>impact speed of anvil=1.2 m/s
.
Why were the Wall Street analysts willing to believe anything about En.docxrichard981
Why were the Wall Street analysts willing to believe anything about Enron? What factors perpetuated the Enron myth? Why were the warnings ignored?.
Solution
Goals for Business Ethics Education
Goals of business ethics education have included the teaching of ethical theory and the
particular contexts unique to making ethical decisions in the workplace (Morse, 1999).
The objective of some courses is to have an effect on student awareness of ethical
issues and attitudes towards ethics, while other courses focus on reasoning ability, with
a third type of course seeking to integrate the two objectives of awareness and
reasoning (McDonald, 2004). Williams & Dewett (2005) contend that business ethics
education should promote students
.
Why the Uniform Commercial Code exists- Is it important-SolutionThe ma (1).docxrichard981
Why the Uniform Commercial Code exists. Is it important?
Solution
The main purposes of the UCC are:
1. To simplify, clarify, and modernize the law governing commercial
transactions.
2. To permit the continued expansion of commercial transactions.
3. To provide for consistency in the law regarding the sale and financing of
personal property in the various jurisdictions (municipalities, counties, and
states).
Yes,It is important
.
why the Uniform Commercial Code exists- Is it important-SolutionThe ma.docxrichard981
why the Uniform Commercial Code exists. Is it important?
Solution
The main purposes of the UCC are:
1. To simplify, clarify, and modernize the law governing commercial
transactions.
2. To permit the continued expansion of commercial transactions.
3. To provide for consistency in the law regarding the sale and financing of
personal property in the various jurisdictions (municipalities, counties, and
states).
Yes,It is important
.
why the existence of electron trapping sites in a crystalline material.docxrichard981
why the existence of electron trapping sites in a crystalline material is desirable if the material is used as a thermoluminescent dosimeter, but undesirable if the material is used as a conventional scintillator
Solution
Thermoluminescent dosimeter (TLD) is a passive radiation detection device that is used for personal dose monitoring or to measure patient dose. Materials exhibiting thermoluminescence in response to ionizing radiation. The ionizing radiation will be active when the existence of electron trapping sites in a crystalline material. But the electron trapping sites are not useful in conventional scintillator because a scintillation detector or scintillation counter is obtained when a scintillator is coupled to an electronic light sensor such as a photomultiplier tube (PMT), photodiode, or silicon photomultiplier.
.
Why saturated suspended weight is less than dry weight and soaked weig.docxrichard981
Why saturated suspended weight is less than dry weight and soaked weight?
Solution
The Soil is made up of four phases namely Air, water ,voids and solid particles. The solid particles and the water present in the soil have both weight and occupy some volume. However, the air that is present in the soil does not have any weight.
in a saturated system of soil, the components of water and solids is present while the air component is completely removed from the soil.
In a dry soil,the component of water is absent while the pores (voids) of the soil is completely filled with air . So the weight of dry soil equals weight of only solids.
In a Soaked soil,the voids are filled u with water. So additional weight of water adds up.
In suspended saturated soil, the Buoyancy forces of water acts against the weight of soil. So i weighs less than the other kinds of soil
.
Why might a small corporation organize as an S corporation- An S corpo.docxrichard981
Why might a small corporation organize as an S corporation? An S corporation (a) does not have to issue stock (b) is not subject to the corporate income tax (c) is the only form of business organization that offers limited liability (d) is the only form of business organization that provides unlimited life. 11. Double taxation refers to the fact that (a) corporations have to pay both state and federal taxes (b) the part of a corporation\'s earnings not distributed as dividends is subject to excess profits taxes (e) corporations pay both income taxes and sales taxes (d) corporations pay income taxes on their earnings, and the earnings that they distribute as dividends are subject to personal income taxes.
Solution
10c11d
.
Why might elemental bromine and iodine be more soluble in dicholormeth.docxrichard981
Why might elemental bromine and iodine be more soluble in dicholormethane than in water?
Why might elemental bromine and iodine be more soluble in dicholormethane than in water?
Solution
according to like dissolve like principle polar compounds soluble in polar solvents only.
non-polar compounds dissolve in non-polar solvents only
Br2 and I2 both are non-polar compunds. dichloromethane also non-polar solvent so
both Cl2 and I2 soluble in dichloromethane then water
.
Why lack of control got Comcast in trouble-Solutionthe media player is.docxrichard981
Why lack of control got Comcast in trouble?
Solution
the media player is experienced many critics and many problems in its entire life.
there are many people who critisize becuase of its policies and procedures, the way they deal the programs and broadcasting.
because of lack of competition they enjoyed high market share earlier, later many players entered and started capturing the market share.
Comcast personally benefited most of the times while using the information, they blackmailed the accused people and gaind personal benefits which legally and ethically not acceptable.
what ever the firm or individual, if they crossed ethics and if they wont respect law, they need to be face allegations and they create problems to society, rather than solutions.
.
Why might a company accept a project or investment with an NPV less th.docxrichard981
Why might a company accept a project or investment with an NPV less than zero?
Solution
A company accept a project or investment with an NPV less than zero due to many a points:
1. If a firm has poor corporate governance, managers may ignore the net present value rule. In order to appear active, management may take on negative NPV projects to give shareholders the illusion of ongoing corporate investment.
2. As the usefulness of net present value relies entirely upon accuracy of the expected income of a project and the discount rate. The company accepting a project of NPV less than zero on the assumption that the discount rate is set too high or expected income is too optimistic which has resulted in negative NPV. They accept the project on positive future.
3. If the Company is going with no business, then it might be tempted to accept a project with NPV less than zero.
.
Why is Pluto so small- compared to the gas giants- Pick the one best a.docxrichard981
Why is Pluto so small, compared to the gas giants? Pick the one best answer.
A.Pluto was so far away from the Sun that it was too cold for gases to condense and create a gas giant
B.Pluto is small right now, but it is still growing: in the far future it will be as large as Neptune
C.Dwarf planets are always smaller than gas giants
D.The density of the building blocks was lower where Pluto formed compared to where Uranus and Neptune formed
E.Pluto is not a planet, rather it is a moon of Neptune that got ejected
Solution
initially pluto was considered planet but then it was discovered that is mass and size is very small so it was then not considered planet so considered as dwarf planet because of its smaller mass and size.
so correct option is C.
.
Why is it important to use multiple performance measurements when eval.docxrichard981
Why is it important to use multiple performance measurements when evaluating the successful operation of responsibility centers?
Solution
Profit is not the only measurement of performance of any enterprise or responsibility centres within an enterprise. There can be many financial and non-financial parameters for the purpose. Financial pameters could be Revenue , Turnover , Contribution Margin , Liquidity etc. Non-finanacial parameters could be Innovations, employee satisfaction , customer satisfaction , corporate social responsibility , corporate governance etc. Only when a performance evaluation takes into account all these parameters , the evaluation is comprehensive and useful.
.
Why does the absorption spectrum of as semiconductor have a sharp-stee.docxrichard981
Why does the absorption spectrum of as semiconductor have a sharp/steep region in the middle and two flat regions on either side? For emission spectrum, why is the emission peak typically sharp (narrow)?
Solution
molecules absorb light to different excited states. Emission comes from the lowest of these states, called the S1 state. When a molecule is excited to a higher state it often ends up in this lowest excited state S1 and then emits radiation. In this case the excitation spectrum is the same as the absorption spectrum. However from a higher excited state a molecule does not have to go to the lowest excited state. It can directly go to the ground state for instance. Then it does not emit radiation. In this case the excitation spectrum only has the lowest energy absoption features.
.
why is it important to understand culturesSolutionIt important to unde.docxrichard981
why is it important to understand cultures
Solution
It important to understand culture so then you can pass down traditions , know about your close friends, you, and your family tree. As well as to know how to respect and appreciate others at what they do .
.....
There are many reasons why understanding different cultures is very important. A reason may be so that you can analyze things from a different point of view, outside you \"box\". Another reason is so that you can better understand why some people act the way they do and why they make certain decision because I think that the way someone gets brought up plays an important role in that. Some other reason is to respect other cultures and just enrich yourself with learning about new traditions etc..
.
Why is goal setting important- What are three benefits- What are three.docxrichard981
Why is goal setting important? What are three benefits?
What are three key behaviors (characteristics) for effective goal setting?
What are goal statements?
What is
Solution
Why is goal setting important? What are three benefits : A jounney is futile if no destination is set in advance. The 3 benefits of goal setting are : clear direction , helps in planning and optimum utilization of resources. What are three key behaviors (characteristics) for effective goal setting? : They are derived from the mission and vision statement of a person or organization , they are long term and they are cherished by the goal setter. What are goal statements? : These are the statements that define the goals of a person or an organization. What is
.
Why is frequency reuse of 1 possible and what is the number of users p.docxrichard981
Why is frequency reuse of 1 possible and what is the number of users per carrier it allows?
Solution
In a low loaded network, the scheduler in each cell has an easy life as the amount of UE(user equipment)\'s simultaneous active is little. But LTE(Long Term Evolution) can prove its superior Frequency Domain Scheduling algorithm\'s (which are eNB Vendor specific) when the network gets loaded and RSRQ gets below -12 dB ... - 16 dB due to high interCell Interference from neighbor cells when looking at a particular serving cell. The Scheduler on its own can schedule the UE\'s in its own cell according to his scheduling algorithm and/or takes InterCell Interference Co-ordination signaling via X2-interface into account and be restricted to schedule cdertain Resource Blocks so that cell edge users are less interfered. This of course can be considered as a soft-reuse which enforces an re-use > 1.
No. of users >1
.
why EI can not be used in determining protein primary structures-Solut.docxrichard981
why EI can not be used in determining protein primary structures?
Solution
EI is considered a hard (high fragmentation) ionization method, since it uses high energetic electrons
to produce ions. This leads to extensive fragmentation, which can be helpful for structure determination of unknown
compounds. EI is the most useful for organic compounds which have a molecular weight below 600.
EI is useful for mass range <1000 Da, But Fragmentation of proteins in the 13000 to 29000Da mass range
observed.
Therefore, EI can not be used in determining protein primary structures
.
Why is anion suppression necessary in ion chromatography-SolutionAnion.docxrichard981
Why is anion suppression necessary in ion chromatography?
Solution
Anion suppression is mandatory in ion chromatography, particularly in anion exchange chromatography. In suppressed ion chromatography, the sample is introduced and flows along with an eluent to the ion exchange column where separation occurs. After the separation, the mixture of ions in the eluent is introduced to a suppressor which exchanges the eluent and counter ions with suppressor derived ions. This exchange of the eluent has two practical advantages:
(i) The background conductivity of the eluent is reduced; this enhances the conductivity of the sample ions for easy detection by a conductivity detector.
(ii) The baseline noise is reduced; this increases the signal to noise ratio and thus, the sensitivity of the instrument.
.
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
Thinking of getting a dog? Be aware that breeds like Pit Bulls, Rottweilers, and German Shepherds can be loyal and dangerous. Proper training and socialization are crucial to preventing aggressive behaviors. Ensure safety by understanding their needs and always supervising interactions. Stay safe, and enjoy your furry friends!
Delivering Micro-Credentials in Technical and Vocational Education and TrainingAG2 Design
Explore how micro-credentials are transforming Technical and Vocational Education and Training (TVET) with this comprehensive slide deck. Discover what micro-credentials are, their importance in TVET, the advantages they offer, and the insights from industry experts. Additionally, learn about the top software applications available for creating and managing micro-credentials. This presentation also includes valuable resources and a discussion on the future of these specialised certifications.
For more detailed information on delivering micro-credentials in TVET, visit this https://tvettrainer.com/delivering-micro-credentials-in-tvet/
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
Why aren-'t all detectors suitable for gradient elution methods in HPL.docx
1. Why aren't all detectors suitable for gradient elution methods in HPLC? You should include an
example of a detection system that works with it, and one that does not, but also give reason for
their success/failure as a gradient elution detector.
Solution
The gradient elution methods used for heavy molecular weight component move faster in mobile
phase of HPLC/Chromatography, and least molecular weight component move slower in
elucidation of chromatography.
The detection in HPLC due to absorbance, In HPLC UV detector and refractive index detector
are commenly used.
UV detector is easy to operate and provide good stability compared to all other detector.
More preferebly. differential refractometer (DRI), or refractive index detector (RI or RID) is
measures the refractive index of an analyte relative to the solvent.
