This presentation described the different kinds of staining procedure and culture media needed for certain type of microorganism

1. BACTERIAL STAINING
AND CULTURE MEDIA

3. 



6. 

1.






9. Reagent Function Result if Gram-positive Result if Gram-negative
Crystal volet Primary stain Purple or Blue Purple or Blue
Gram’s iodine Mordant Purple or Blue Purple or Blue
Acetone or 95% alcohol Decolorizer Purple or Blue Colorless
Safranin Counterstain or
secondary stain
Purple or Blue Red or Pink

10. 

a.
b.

13. Reagent Function Result
Ziehl-Neelsen Kinyoun Acid-fast Non-acid fast
Carbol fuchsin Carbol fuchsin Primary stain Red or Pink Red or Pink
Acid alcohol Acid alcohol Decolorizer Red Colorless
Methylene Blue Malachite Green Counterstain or
Secondary Stain
Ziehl-Neelsen:
red
organism/blue
background
Kinyoun: red
organism/green
background
Ziehl-Neelsen:
blue
organism/blue
background
Kinyoun: green
organism/green
background

15. 







18. 




19. 




20. 


21. 


a.
b.

22. 1.




23. 



24. 1.



25. 



27. 


28. 



29. 


30. 




31. 




32. 



