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Culture Media - Prac. Microbiology

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Culture Media - Prac. Microbiology

  1. 1. Bacteriological Culture Media
  2. 2. Culture media provide optimal conditions for growth & multiplication of bacteria.
  3. 3. BACTERIOLOGICAL LOOP
  4. 4. INOCULATION ON CULTURE PLATES
  5. 5. According to the physical state media may be: Fluid Media Solid Media Growth is visualized as Turbidity Colonies (macroscopic products of 20-30 cell divisions of a single bacterium on a solid medium)
  6. 6. Plating-out technique  Procedure: Loopful of the specimen Specimen
  7. 7. Solid media are preferable:  Isolation in pure culture.  Identification (colony morphology, effect on medium).
  8. 8. Types of culture media: 1) Simple media 2) Enriched media 3) Selective media 4) Enrichment media 5) Indicator media 6) Selective and indicator media
  9. 9. 1- SIMPLE MEDIA
  10. 10. 1- SIMPLE MEDIA a- Nutrient broth Meat Extract
  11. 11. SEA WEEDS Sea weeds
  12. 12. AGAR POWDER
  13. 13. 1- SIMPLE MEDIA b- Nutrient agar Nutrient Broth + 2% agar-agar
  14. 14. 1-SIMPLE MEDIA Staphylococcus aureus on nutrient agar (golden yellow endopigment)
  15. 15. 1- SIMPLE MEDIA Ps. aeruginosa on nutrient agar (greenish exopigment)
  16. 16. 1-SIMPLE MEDIA Proteus on nutrient agar (swarming growth)
  17. 17. 2-ENRICHED MEDIA a- Blood agar ( also Indicator Medium) Nutrient agar + 5-10% Blood
  18. 18. 2- ENRICHED MEDIA Beta hemolysis on blood agar
  19. 19. 2- ENRICHED MEDIA Alpha hemolysis on blood agar
  20. 20. 2- ENRICHED MEDIA Non hemolytic organism on blood agar
  21. 21. 2- ENRICHED MEDIA Blood agar
  22. 22. 2- ENRICHED MEDIA b-Chocolate agar Heated Blood agar Haemoglobin Heat Haematin
  23. 23. 3-SELECTIVE MEDIA Lowenstein Jensen medium for M. tuberculosis Beaten Eggs + Mineral salts + Malachite Green
  24. 24. 4- ENRICHMENT MEDIA Selenite F Broth
  25. 25. 5- Differential (indicator) media Triple Sugar Iron (TSI) agar TSI agar
  26. 26. 6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA MacConkey’s medium Bile Salts + Lactose + Neutral Red
  27. 27. 6- SELECTIVE INDICATOR (DIFFERENTIAL) MEDIA MacConkey’s medium
  28. 28. Blood Culture For isolation of bacteria from blood samples in cases of bacteraemia.
  29. 29. Blood culture technique (2) Added to 50 – 100 ml fluid medium (broth) (1) 5 – 10 ml blood (4) Bacterial Growth (3) Subculture on Solid medium
  30. 30. Why use Large volume of broth??? 1q111 1- Reduction of conc. of antibacterial constituents in blood 2- Multiplication of bacteria 3- Neutralization of antibiotics
  31. 31. Anaerobic Cultivation -For obligate anaerobes Achieved by: - exclusion of oxygen - addition of reducing substance
  32. 32. ANAEROBIC MEDIA a- Robertson’s cooked meat medium
  33. 33. ANAEROBIC MEDIA b- Thioglycollate Broth
  34. 34. GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION
  35. 35. GAS PAK SYSTEM FOR ANAEROBIC CULTIVATION
  36. 36. MCQs
  37. 37. 1) MacConkey’s medium: a) Is an enriched medium. b) Is an enrichment medium. c) Contains phenol red as a pH indicator. d) Gives pink colonies by lactose fermenting bacteria. e) Gives green colonies by lactose non-fermenting bacteria.
  38. 38. 2) Blood culture technique is used to diagnose: a) Sore throat b) Conjunctivitis c) Skin infection d) Sinusitis e) Bacteraemia
  39. 39. 3) All are correct about enrichment media except: a)They are solid media. b) Blood agar is not considered an enrichment medium. c)They have selective properties. d)They help multiplication of certain species and suppress others. e)They include selenite broth.
  40. 40. 4) All are correct about chocolate agar EXCEPT: a) It is an enrichment medium. b) It contains heated blood. c) It is used to grow fastidious organisms. d) It is an enriched medium. e) It is brown in colour.
  41. 41. 5) One of the following is used to grow strict anaerobes: a) Selenite broth b) MacConkey’s medium c) Blood agar d) Lowenstein-Jensen medium e) Robertson’s cooked meat medium
  42. 42. 6) When strict anaerobes are grown in the Gaspak system: a)They must be inoculated on cooked meat medium. b)They can be grown on blood agar plates. c) 50% carbon dioxide must be added. d) The hydrogen released acts as a catalyst. e)The palladium in the system acts as a nutrient.
  43. 43. Thank You For Your Attention

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