5. According to the physical state
media may be:
Fluid Media
Solid Media
Growth is visualized as
Turbidity
Colonies
(macroscopic products of 20-30 cell divisions of
a single bacterium on a solid medium)
7. Solid media are preferable:
Isolation in pure culture.
Identification
(colony morphology,
effect on medium).
8. Types of culture media:
1) Simple media
2) Enriched media
3) Selective media
4) Enrichment media
5) Indicator media
6) Selective and indicator media
29. Blood culture technique
(2)
Added to
50 – 100 ml
fluid medium
(broth)
(1)
5 – 10 ml blood
(4)
Bacterial
Growth
(3)
Subculture on
Solid medium
30. Why use Large volume of broth???
1q111
1- Reduction of conc. of antibacterial
constituents in blood
2- Multiplication of bacteria
3- Neutralization of antibiotics
37. 1) MacConkey’s medium:
a) Is an enriched medium.
b) Is an enrichment medium.
c) Contains phenol red as a pH indicator.
d) Gives pink colonies by lactose fermenting
bacteria.
e) Gives green colonies by lactose non-fermenting
bacteria.
38. 2) Blood culture technique is used to
diagnose:
a) Sore throat
b) Conjunctivitis
c) Skin infection
d) Sinusitis
e) Bacteraemia
39. 3) All are correct about enrichment media
except:
a)They are solid media.
b) Blood agar is not considered an enrichment
medium.
c)They have selective properties.
d)They help multiplication of certain species and
suppress others.
e)They include selenite broth.
40. 4) All are correct about chocolate agar
EXCEPT:
a) It is an enrichment medium.
b) It contains heated blood.
c) It is used to grow fastidious organisms.
d) It is an enriched medium.
e) It is brown in colour.
41. 5) One of the following is used to grow strict
anaerobes:
a) Selenite broth
b) MacConkey’s medium
c) Blood agar
d) Lowenstein-Jensen medium
e) Robertson’s cooked meat medium
42. 6) When strict anaerobes are grown in the
Gaspak system:
a)They must be inoculated on cooked meat medium.
b)They can be grown on blood agar plates.
c) 50% carbon dioxide must be added.
d) The hydrogen released acts as a catalyst.
e)The palladium in the system acts as a nutrient.
