in this ppt tell us how EGFR growth factor express in cancerous cells....

1. EGFR:- epidermal growth factor receptor

2.  →EGFR is trans membrane glycoprotein and
extracellular ligand binding domain .it is
present on surface of cancer cells.

3.  →Lung cancer occurs when a malignant
(cancerous) tumour grows inside the lungs

4.  Smoking
 Radon gas
 Air pollution
 Genetics
 Some metals (Cadmium, Arsenic, etc.)

5. SMALL CELL NON SMALL CELL
 →Usually start near the
center of the chest in
the bronchi .
→It tend to grow and
spread much faster
than non small cell
cancer
 →Adenocarcinoma is a
slow growing in lung
cancer usually
discovered in outer
area of lung
→large cell carcinoma
occur anywhere in lung
and it usually growth
and spread at rapid
rate

6.  → The EGFR gene is located on the short arm of
chromosome 7 and trans membrane growth factor receptor
bind with TK(Tyrosine kinase) activity.
The TK is the part of the protein, located inside the cell,
which “switches on” when a growth factor (or ligand) from
outside the cell binds to the outside portion of the EGFR. This
switch,, allows the EGFR to signal the cell to grow and
survive.

7.  Transferred 200ul of whole blood into an Micro Cetrifuge Tube.
 Added 200ul of red cell lysis solution.Vortex it for few seconds
for mixing.
 Add 20ul Proteinase k. and then again vortex it.
 Add 4ul RNAse and then incubate at 56°C for 10min.
 Add 200ul ethanol to the above tube and then vortex it.
 Now add the above mix to new fresh column.
 Centrifuge at 8000rpm for a minute.
 Now add 500ul wash buffer 1 and then again centrifuge at
8000rpm for 1min.
 Add 500ul wash buffer 2 and then centrifuge at 14000rpm for
3min.
 Transferred the supernatant to a clean microfuge tube and pellet
was discarded.
 Add 100ul AE Buffer to the tube.
 Centrifuge at 8000rpm for 1min.
 Now take O.D. of the Sample DNA using spectrophotometer.

8.  Polymerase chain reaction (PCR) is a
technique used in molecular
biology to amplify a single copy or a few
copies of a piece of DNA.
 It is an easy tool to amplify a focused
segment of DNA, useful for such purposes as
the diagnosis and monitoring of genetic
diseases and studying the function of a
targeted segment of DNA.

11.  There were 10 Patients who were suspected to be the
Cancer Patients were studied during the one month
training session.
 All of them were males having the average age of 60
Years.Out of these 10 Patients of Lung Cancer Five
patients are the chronic current smokers and Four of
them are Ex-smokers and intrestingly only a single
patient is a Non- smoker.
 The current smoker smokes on average 35 Beedies or
Cigarettes per day. Of those who quit up smoking
smoked on average 45 packs per year of Beedies or
Cigarettes.
 Out of 10 Patients 6 Patients were Positively tested
for the chronic disease of Lung Carcinoma.