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HANDLING OF SEGREGATION
POPULATION: BULK BREEDING
METHOD
DR. RAJENDRAGOUDA PATIL
ASSISTANT PROFESSOR
GENETICS AND PLANT BREEDING
SCHOOL OF AGRICULTURAL SCIENCES AND TECHNOLOGY (SAST), NMIMS,
MPTP SHIRPUR
• Hybridization: crossing between genetically dissimilar plants.
1. Intervarietal (two varieties or genotypes of same species)
2. Interspecific (two species of the same genus)
3. Intergeneric (two genera of the same family)
• Hybridization uses- 3 ways
• To combine several desirable traits in to single genotype
• To create vast genetic variability
• To develop hybrid varieties for commercial cultivation
• Methods to handle segregating population
• Pedigree breeding method
• Back cross breeding method
• Bulk breeding method
BULK BREEDING METHOD
• In this method F2 and subsequent generations are grown in
bulk up to F5 without artificial selection is known as Bulk
breeding method
• This method is given by Nilson Ehle in 1908.
• Also known as mass method or population method or
evolutionary method of crop improvement method.
• After F6 generation individual plant selection is practiced.
FEATUR
ES• Mainly used to self pollinated crops (when both the parents
have good agronomic traits)
• Population handled by bulk method (F1 to F5) and IPS from F6
onwards. Usually 5-6 years of bulking practiced
• In this method natural selection (bulking period) and artificial
selection (IPS) operates
• It changes gene frequencies in population, hence it is called as
“evolutionary method of crop improvement”
• Genetic constitution: homozygous and homogenous
PROCEDU
RE
• Steps:
1. Bulking period (F2-F5)
2. Progeny selection and isolation of superior progeny (F6-F8)
3. Multilocation trials of superior progeny (F9-F14)
4. Release of best progeny as variety
Variety A Variety B Parent
s
F1
F2
F3
F4
F5
Bulk
plot
Bulk
plot
Bulk
plot
Bulk
plot
Bulk
plot
BulkingPeriod
- - - - - - - - - - - - - - -
- - - - - - - - - - - - - - -
- - - - - - - - - - - - - - -
- - - - - - - - - - - - - - -
- - - - - - - - - - - - - - -
- - - - - - - - - - - - - - -
- -
F5
F6
F7
F8
F9-F14
Space
Planted
Preliminary yield
trials
Head to
rows
Yield
trials
Progenyselection
(Multi-location tastings)
Release of Variety
MERITS AND
DEMERITS
SN Merits Demerits
1. Simple, convenient and less
expensive
Longer time to develop new
variety
2. Natural selection (deletes
undesirable traits)
Due to short term bulking,
natural selection has little
effective
3. Chance of obtaining
transgressive segregants more
than pedigree method
Large number of progenies
have to be selected at the end
of bulking
4. No chance of gene elimination There is chance of natural
selection acts against superior
traits
5. Progeny selection gives better
variety
6. No pedigree record maintained
ACHIEVEMEN
TS
• Barley in USA: ARIVAL, BEECHER,GLACER & GEM (Altas X
Vaughn)
• Brown Mustard: Narendra Rai
SINGLE SEED DESCENT METHOD
(SSD)
• A breeding method used for segregating populations of self
pollinated crops in which plant are advanced by single seeds
from on generation to other referred as SSD
• Suggested by Goulden (1939)
• Later it was applied by Grafius (1965) in oats, Brim (1966) in
Soybean.
• It is modified bulk breeding method
Variety A Variety B Parent
s
F1
F3
Bulk
plot
Bulk
plot
F2
F5
IPS F6 - F8
MERITS AND
DEMERITS
SN Merits Demerits
1. Simple, convenient and less
expensive
Not provide opportunity for to
select superior plant till F5
2. Crosses can be evaluated
through this method
Frequency of getting desirable
genotypes in advanced
generation is reduced
3. Able to remain considerable
variability
Applicable to self-pollinated
crops.
4. The material can be advanced
by growing the same in green
house

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Bulk Breeding Method

  • 1. HANDLING OF SEGREGATION POPULATION: BULK BREEDING METHOD DR. RAJENDRAGOUDA PATIL ASSISTANT PROFESSOR GENETICS AND PLANT BREEDING SCHOOL OF AGRICULTURAL SCIENCES AND TECHNOLOGY (SAST), NMIMS, MPTP SHIRPUR
  • 2. • Hybridization: crossing between genetically dissimilar plants. 1. Intervarietal (two varieties or genotypes of same species) 2. Interspecific (two species of the same genus) 3. Intergeneric (two genera of the same family) • Hybridization uses- 3 ways • To combine several desirable traits in to single genotype • To create vast genetic variability • To develop hybrid varieties for commercial cultivation • Methods to handle segregating population • Pedigree breeding method • Back cross breeding method • Bulk breeding method
  • 3. BULK BREEDING METHOD • In this method F2 and subsequent generations are grown in bulk up to F5 without artificial selection is known as Bulk breeding method • This method is given by Nilson Ehle in 1908. • Also known as mass method or population method or evolutionary method of crop improvement method. • After F6 generation individual plant selection is practiced.
  • 4. FEATUR ES• Mainly used to self pollinated crops (when both the parents have good agronomic traits) • Population handled by bulk method (F1 to F5) and IPS from F6 onwards. Usually 5-6 years of bulking practiced • In this method natural selection (bulking period) and artificial selection (IPS) operates • It changes gene frequencies in population, hence it is called as “evolutionary method of crop improvement” • Genetic constitution: homozygous and homogenous
  • 5. PROCEDU RE • Steps: 1. Bulking period (F2-F5) 2. Progeny selection and isolation of superior progeny (F6-F8) 3. Multilocation trials of superior progeny (F9-F14) 4. Release of best progeny as variety
  • 6. Variety A Variety B Parent s F1 F2 F3 F4 F5 Bulk plot Bulk plot Bulk plot Bulk plot Bulk plot BulkingPeriod
  • 7. - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - F5 F6 F7 F8 F9-F14 Space Planted Preliminary yield trials Head to rows Yield trials Progenyselection (Multi-location tastings) Release of Variety
  • 8. MERITS AND DEMERITS SN Merits Demerits 1. Simple, convenient and less expensive Longer time to develop new variety 2. Natural selection (deletes undesirable traits) Due to short term bulking, natural selection has little effective 3. Chance of obtaining transgressive segregants more than pedigree method Large number of progenies have to be selected at the end of bulking 4. No chance of gene elimination There is chance of natural selection acts against superior traits 5. Progeny selection gives better variety 6. No pedigree record maintained
  • 9. ACHIEVEMEN TS • Barley in USA: ARIVAL, BEECHER,GLACER & GEM (Altas X Vaughn) • Brown Mustard: Narendra Rai
  • 10. SINGLE SEED DESCENT METHOD (SSD) • A breeding method used for segregating populations of self pollinated crops in which plant are advanced by single seeds from on generation to other referred as SSD • Suggested by Goulden (1939) • Later it was applied by Grafius (1965) in oats, Brim (1966) in Soybean. • It is modified bulk breeding method
  • 11. Variety A Variety B Parent s F1 F3 Bulk plot Bulk plot F2 F5 IPS F6 - F8
  • 12. MERITS AND DEMERITS SN Merits Demerits 1. Simple, convenient and less expensive Not provide opportunity for to select superior plant till F5 2. Crosses can be evaluated through this method Frequency of getting desirable genotypes in advanced generation is reduced 3. Able to remain considerable variability Applicable to self-pollinated crops. 4. The material can be advanced by growing the same in green house