1. HANDLING OF SEGREGATION
POPULATION: BULK BREEDING
METHOD
DR. RAJENDRAGOUDA PATIL
ASSISTANT PROFESSOR
GENETICS AND PLANT BREEDING
SCHOOL OF AGRICULTURAL SCIENCES AND TECHNOLOGY (SAST), NMIMS,
MPTP SHIRPUR
2. • Hybridization: crossing between genetically dissimilar plants.
1. Intervarietal (two varieties or genotypes of same species)
2. Interspecific (two species of the same genus)
3. Intergeneric (two genera of the same family)
• Hybridization uses- 3 ways
• To combine several desirable traits in to single genotype
• To create vast genetic variability
• To develop hybrid varieties for commercial cultivation
• Methods to handle segregating population
• Pedigree breeding method
• Back cross breeding method
• Bulk breeding method
3. BULK BREEDING METHOD
• In this method F2 and subsequent generations are grown in
bulk up to F5 without artificial selection is known as Bulk
breeding method
• This method is given by Nilson Ehle in 1908.
• Also known as mass method or population method or
evolutionary method of crop improvement method.
• After F6 generation individual plant selection is practiced.
4. FEATUR
ES• Mainly used to self pollinated crops (when both the parents
have good agronomic traits)
• Population handled by bulk method (F1 to F5) and IPS from F6
onwards. Usually 5-6 years of bulking practiced
• In this method natural selection (bulking period) and artificial
selection (IPS) operates
• It changes gene frequencies in population, hence it is called as
“evolutionary method of crop improvement”
• Genetic constitution: homozygous and homogenous
5. PROCEDU
RE
• Steps:
1. Bulking period (F2-F5)
2. Progeny selection and isolation of superior progeny (F6-F8)
3. Multilocation trials of superior progeny (F9-F14)
4. Release of best progeny as variety
6. Variety A Variety B Parent
s
F1
F2
F3
F4
F5
Bulk
plot
Bulk
plot
Bulk
plot
Bulk
plot
Bulk
plot
BulkingPeriod
8. MERITS AND
DEMERITS
SN Merits Demerits
1. Simple, convenient and less
expensive
Longer time to develop new
variety
2. Natural selection (deletes
undesirable traits)
Due to short term bulking,
natural selection has little
effective
3. Chance of obtaining
transgressive segregants more
than pedigree method
Large number of progenies
have to be selected at the end
of bulking
4. No chance of gene elimination There is chance of natural
selection acts against superior
traits
5. Progeny selection gives better
variety
6. No pedigree record maintained
9. ACHIEVEMEN
TS
• Barley in USA: ARIVAL, BEECHER,GLACER & GEM (Altas X
Vaughn)
• Brown Mustard: Narendra Rai
10. SINGLE SEED DESCENT METHOD
(SSD)
• A breeding method used for segregating populations of self
pollinated crops in which plant are advanced by single seeds
from on generation to other referred as SSD
• Suggested by Goulden (1939)
• Later it was applied by Grafius (1965) in oats, Brim (1966) in
Soybean.
• It is modified bulk breeding method
11. Variety A Variety B Parent
s
F1
F3
Bulk
plot
Bulk
plot
F2
F5
IPS F6 - F8
12. MERITS AND
DEMERITS
SN Merits Demerits
1. Simple, convenient and less
expensive
Not provide opportunity for to
select superior plant till F5
2. Crosses can be evaluated
through this method
Frequency of getting desirable
genotypes in advanced
generation is reduced
3. Able to remain considerable
variability
Applicable to self-pollinated
crops.
4. The material can be advanced
by growing the same in green
house