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HANDLING OF SEGREGATION
POPULATION: PEDIGREE METHOD
DR. RAJENDRAGOUDA PATIL
ASSISTANT PROFESSOR
GENETICS AND PLANT BREEDING
SCHOOL OF AGRICULTURAL SCIENCES AND TECHNOLOGY (SAST), NMIMS,
MPTP SHIRPUR
• Hybridization: crossing between genetically dissimilar plants.
1. Intervarietal (two varieties or genotypes of same species)
2. Interspecific (two species of the same genus)
3. Intergeneric (two genera of the same family)
• Hybridization uses- 3 ways
• To combine several desirable traits in to single genotype
• To create vast genetic variability
• To develop hybrid varieties for commercial cultivation
• Methods to handle segregating population
•Pedigree breeding method
• Back cross breeding method
• Bulk breeding method
INTRODUCTI
ON
• The method was described by H H Lowe in 1927
• Pedigree refers to record of the ancestry of an individual selected
plant
• The pedigree may be defined as a description of the ancestry of an
individual and generally goes back to some distant ancestors
• It will helps to know about the parents, grand parents and great
grand parents so on…
Pedigree Method
• Helps in finding out if two individuals are related by
descent their ancestor and likely to have common genes
• It is method of genetic improvement of self pollinated
crops.
• Superior plants are selected from segregating generation
and proper record of ancestry of selected plants are
maintained in each generation
• Each progeny in every generation can be tract back to the
F2 plants from which progeny originated such record is
known as pedigree-record
FEATURE
S
• Application: applicable to self pollinated crop species and used
to develop varieties, used for polygenic traits, in cross
pollinated crops used to develop inbred lines.
• Record: it maintained ancestors record
• Selection: Artificial selection/ human selection, Natural
selection is allowed in modified mass pedigree selection
• Time: 14-15 years
• Genetic nature: homozygous and homogeneous
Variety A Variety B
- - - - - - - -
- - - - - - - -
- - - - - - - -
- - - - - - - -
- - - - - - - -
- - - - - - - -
Parent
s
F1
F2
F3
F4
F5
Bulk
plot
Space planting
Plant to
rows
Family
selection
Family
selection
Cont.
…
F6
F7
F8
F9-F12
Progeny
selection
Progeny
selection
Progeny
selection
Preliminary
yield trial
Yield trial
F5
MASS PEDIGREE METHOD
• Modified Pedigree method, was proposed by Harrington (1937)
Growing of segregating population by bulk or mass selection
when conditions are unfavorable
Use of progeny testing/pedigree method when conditions are
favorable
Harlan applied in Cotton
MERITS AND DEMERITS
SN Merits Demerits
1. Provides mode of
inheritance of various
trait’s information
Material becomes large,
difficult to handle.
2. There is chance of
recovering transgressive
segregates
The continuous record
has to maintain
3. Takes 14-15 years
compared to bulk
method
Large number of progeny
are rejected
4. The value of selected
plants ascertained by
ACHIEVEMEN
TS
• Rice- ADT-25, Jaya, Padma, Sabarmati, Bala
• Wheat- NP-52, 120, 125, DWR 39, WH 331
• Cotton- Digvijay, Abhadita, Sharda, Sahana
• Pigeon pea- T21 and Prabhat
• Green gram: Sheela
• Chick pea: Radhey
• Sorghum: Co 18
• Tobacco: NP 222

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Pedigree Breeding Method

  • 1. HANDLING OF SEGREGATION POPULATION: PEDIGREE METHOD DR. RAJENDRAGOUDA PATIL ASSISTANT PROFESSOR GENETICS AND PLANT BREEDING SCHOOL OF AGRICULTURAL SCIENCES AND TECHNOLOGY (SAST), NMIMS, MPTP SHIRPUR
  • 2. • Hybridization: crossing between genetically dissimilar plants. 1. Intervarietal (two varieties or genotypes of same species) 2. Interspecific (two species of the same genus) 3. Intergeneric (two genera of the same family) • Hybridization uses- 3 ways • To combine several desirable traits in to single genotype • To create vast genetic variability • To develop hybrid varieties for commercial cultivation • Methods to handle segregating population •Pedigree breeding method • Back cross breeding method • Bulk breeding method
  • 3. INTRODUCTI ON • The method was described by H H Lowe in 1927 • Pedigree refers to record of the ancestry of an individual selected plant • The pedigree may be defined as a description of the ancestry of an individual and generally goes back to some distant ancestors • It will helps to know about the parents, grand parents and great grand parents so on… Pedigree Method
  • 4. • Helps in finding out if two individuals are related by descent their ancestor and likely to have common genes • It is method of genetic improvement of self pollinated crops. • Superior plants are selected from segregating generation and proper record of ancestry of selected plants are maintained in each generation • Each progeny in every generation can be tract back to the F2 plants from which progeny originated such record is known as pedigree-record
  • 5. FEATURE S • Application: applicable to self pollinated crop species and used to develop varieties, used for polygenic traits, in cross pollinated crops used to develop inbred lines. • Record: it maintained ancestors record • Selection: Artificial selection/ human selection, Natural selection is allowed in modified mass pedigree selection • Time: 14-15 years • Genetic nature: homozygous and homogeneous
  • 6. Variety A Variety B - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Parent s F1 F2 F3 F4 F5 Bulk plot Space planting Plant to rows Family selection Family selection
  • 8. MASS PEDIGREE METHOD • Modified Pedigree method, was proposed by Harrington (1937) Growing of segregating population by bulk or mass selection when conditions are unfavorable Use of progeny testing/pedigree method when conditions are favorable Harlan applied in Cotton
  • 9. MERITS AND DEMERITS SN Merits Demerits 1. Provides mode of inheritance of various trait’s information Material becomes large, difficult to handle. 2. There is chance of recovering transgressive segregates The continuous record has to maintain 3. Takes 14-15 years compared to bulk method Large number of progeny are rejected 4. The value of selected plants ascertained by
  • 10. ACHIEVEMEN TS • Rice- ADT-25, Jaya, Padma, Sabarmati, Bala • Wheat- NP-52, 120, 125, DWR 39, WH 331 • Cotton- Digvijay, Abhadita, Sharda, Sahana • Pigeon pea- T21 and Prabhat • Green gram: Sheela • Chick pea: Radhey • Sorghum: Co 18 • Tobacco: NP 222