What type of bonds are being formed or broken during denaturation? (Check all that are correct) phosphodiester bonds are formed hydrogen bonds are broken hydrogen bonds are formed no bonds are formed or broken phosphodiester bonds are broken Solution Answer: Hydrogen bonds are broken Denaturation is the loosing of the quarternary, tertiary or secondary structures of proteins or DNA molecule of its native form by applying external energy like heat or using compounds like salts or organic compounds. Hydrogen bonds are weak non-covalent bonds mainly involve in the formation of secondary strucures of DNA and proteins. It need less energy to break these bonds than covalent bonds like phosphodiester bonds..