Important concepts for the FL Bio EOC Assessment; outlined in a digital notebook format.

1. Biology EOC Review

2. Science Methods
• Steps used to solve a problem
• Observation
• Questioning and stating problems
• Hypothesizing
• Experimenting – including a control and experimental group
IV – independent variable
DV – dependent variable
• Tables and Graphs
• IV on x-axis and DV on y-axis of a graph
Ex) Effects of pH on Tadpole Survival
IV – pH
DV-Number of Tadpoles

3. Characteristics of Life
All living things exhibit several basic life characteristics:
•Cellular organization
*unicellular – one celled
* multicellular – many celled with levels of
organization (cellstissuesorgans systems organism)
•Reproduction
*asexual – offspring are genetic clones of parent
*sexual – offspring have genetic variation from parents
•Metabolism
* energy is required for life processes
* autotrophs make their own food (photosynthesis/chemosynthesis)
* heterotrophs eat other organisms for food
•Homeostasis
* maintenance or regulation of body conditions such as body
temperature, blood sugar level, water balance
•Heredity
* DNA – deoxyribonucleic acid – is the genetic material that codes
for proteins of all organisms. The genetic code is “universal”
•Response to stimuli
* responding to the biotic and abiotic factors in the environment are
key to survival
•Growth and Development
* growth – increase in the amount of living matter either by cell
division or cell enlargement
* development – any change from conception to death -
embryonic, puberty, aging
•Evolutionary Adaptation
* adaptations – structures, behaviors, or processes that aid in an
organisms survival are passed on from parent to offspring.

4. Chemistry
• Organisms are composed of organic compounds – carbon
containing compounds that can be very large macromolecules
• Macromolecules are often built by dehydration synthesis and
polymerization
• Four main types
1) Carbohydrates – composed of monosaccharides primarily glucose
2) Lipids – composed of fatty acids joined to glycerol and sometimes
phosphate groups, can also include the steroids
3) Proteins – composed of amino acids (20 different types) – do most of
the work in organisms and are major structural components
4) Nucleic Acids – are composed of nucleotides – either DNA or RNA
• Metabolism – is the chemistry of life – all metabolism is controlled
by the action of enzymes
• Enzymes are proteins that function to speed up chemical reactions
in the cell. They have a specific shape and interact with a specific
substrate which binds at the active site.
• Enzymes are reusable and are not changed during a chemical
reaction. They can be damaged at temperature and pH extremes.

5. Ecology• Ecology – is the study of interactions between organisms and the
environment
• Levels of Organization
BiosphereBiomesEcosystem CommunityPopulationOrganism
• We study an organisms habitat, niche, and trophic level
• Populations – are members of the same species living in the same
place at the same time with the potential to interbreed
Population growth – exponential (J-shape) and logistic (S-Shape)
* Limited by factors like disease and competition that are density-
dependent or by density-independent factors like natural disaster.
* Carrying capacity is seen in logistic growth – the maximum number
the environment can support
Community Interactions
* Competition – intraspecific (same species) or interspecific (diff sp)
* Symbiosis – parasitism, commensalism, and mutualism
* Succession – both primary (bare rock) and secondary (soil)
Ecosystem Level – food chains and webs and matter recycling

6. Cells
• Cell theory - 3 parts
1) cells are basic unit of life
2) cells come from existing cells
3) all organisms are composed of cells
• Prokaryotic versus Eukaryotic
A) simple A) complex
B) has no nucleus B) has a MB nucleus
C) has no MB organelles C) has MB organelles
D) includes bacteria D) includes protists, fungi,
plants, and animals
• Organelles – compartments for carrying out specific jobs / chemical
reactions
1) chloroplast – photosynthesis
2) mitochondria – cellular respiration
3) ribosomes – protein synthesis
4) vacuoles – storage
5) nucleus – contains DNA and
controls cell actions
6) nucleolus – site of ribosome formation
• Plant versus Animal
A) has cell wall A) no cell wall
B) has chloroplasts/plastids B) has no
plastids/chloroplasts
C) has large vacuole C) has small vacuoles

7. Cellular Transport
• Plasma membrane controls homeostasis (balance)
• Structure – composed of a phospholipid bilayer with
embedded proteins “gates”
• Function – acts as a selectively permeable boundary
around the cell
• Types of Passive Transport – no energy required
1) Diffusion – moves substances from high to low concentrations
down their concentration gradient
2) Osmosis – the diffusion of water from high to lower water
concentrations down its concentration gradient
Ex) cell in salt water – shrivels Ex) cell in fresh water swells
3) Facilitated diffusion – movement of a substance down its
concentration through a transport protein channel
• Active Transport – requires energy – moves substances against the
concentration gradient from low to high concentrations

8. Photosynthesis
• The process used by producers to convert sunlight to chemical
energy in glucose
• Overall equation: 6CO2 + 6H2O  C6H12O6 + 6O2
• Occurs in the palisade layer of leaves (yellow layer under the upper
epidermis)
• Large numbers of chloroplasts are found in these mesophyll cells.
• Chloroplasts are the cellular site of photosynthesis. The light
reaction of photosynthesis occurs on the inner membrane called the
thylakoid. The dark reaction (aka Calvin Cycle) occurs in the
stroma
• Pigments absorb light energy
• Chlorophyll / carotenoids
Input Output
Light Reaction light, water O2, ATP
NADPH
Dark Reaction ATP, CO2 GLUCOSE
NADPH

9. Cellular Respiration
• Cellular respiration is the process by which organisms break down
food to release its energy. This energy is then stored in ATP
(Adenosine triphosphate)
• Three parts to ATP
1) adenine (Nbase)
2) ribose (5-C sugar)
3) 3 phosphates (high energy)
• ATP/ADP cycle – when energy
is needed for cell work ATP
loses a phosphate to become ADP
• Overall equation: C6H12O6 + 6O2  6CO2 + 6H2O + 38 ATP
• Respiration can be aerobic or anaerobic
Aerobic Anaerobic
O2 required no O2 required
most organisms are aerobes few anaerobes (yeast/bacteria)
38 ATP 2 ATP
3 steps – glycolysis, Krebs cycle, 2 steps – glycolysis and
electron transport fermentation (alcoholic and
lactic acid)
• Glycolysis is the first step of both
forms of respiration and occurs in
the cytoplasm
• If no oxygen is present after glycolysis,
then fermentation occurs
• If oxygen is present, then the Krebs cycle
and e-transport occur in the mitochondria

10. DNA, RNA, and Protein
Synthesis
• DNA and RNA are composed of nucleotides
DNA RNA____________
Deoxyribose Ribose
A, C, G A, C, G
Thymine Uracil
Double helix Single helix
Codes for proteins/RNA Copy of DNA info
• Replication – the process used by cells to copy DNA – enzyme
unzips DNA and each side of the ladder acts as a template for the
building of the new half. Use the N-base paring rules : A-T ; C-G
EX) TACGGAC (old strand)
ATGCCTG (new strand
• Transcription – the process of making RNA from DNA
EX) TACGGAC (template DNA strand)
AUGCCUG (RNA built)
• 3 Types of RNA have a
role in protein synthesis
1) mRNA – messenger-blueprint
for how to build protein
2) tRNA – transfer - carries amino
acids to ribosome
3) rRNA – ribosomal – makes up
a ribosome
• Translation – the process of
of building a protein by matching
Codons in mRNA to anticodons
of tRNA (use codon chart)

11. Reproduction
• Reproduction is a fundamental characteristic of life
• Propagates your species
• 2 form: asexual and sexual
Asexual Sexual____________
-1 parent -2 parents (usually)
-No gametes -Fusion of gametes
-Offspring are genetically identical -Offspring genetically unique
to the parent (clones)
-Fast, efficient, less energy -Slower, less efficient, more
energy
-No variation -Huge amounts of variation
-Stable Environment -Changing Environment
• Asexual Strategies
1) binary fission
2) budding
3) fragmentation/fission
• Sexual strategies
1) Internal fertilization
Copulation (vagina/penis) –
sperm meets egg in female
2) External fertilization
Spawning – eggs and sperm
released into the environment
usually aquatic
• In humans fertilization occurs
in the fallopian tube.

12. Cell Division
• Haploid – having one set of chromosomes (n) – gametes – sperm/egg
• Diploid – having two sets of chromosomes (2n) – body cells – one set
is maternal and one is paternal
• The cell cycle – Interphase – growth
- Mitosis – division
• Mitosis creates diploid cells and is for
the purpose of tissue repair and growth
in animals
• DNA coils to form chromosomes
during cell division
• Stages of the cell cycle ( see diagram)
Interphase, Prophase, Metaphase,
Anaphase, Telophase, Cytokinesis
• Meiosis – cell division that creates 4
haploid cells called gametes – aka –
reduction division
• Meiosis involves 2 divisions – Meiosis I
and Meiosis II
• Meiosis I has some special events:
• In Prophase I homologous chromosomes
pair up and crossing over occurs. This
recombination increases genetic
variation for the species
• Metaphase I – Pairs line up
• Anaphase I – pairs are separated
• Meiosis II is similar to mitosis

13. Simple Genetics
• Gregor Mendel worked with pea plants to learn the basic patterns of
inheritance.
• Phenotype – what the organism looks like
• Genotype – the gene combination – either Homozygous (TT or tt)
or Heterozygous (Tt)
Monohybrid Cross – follows 1 trait through several generations
P(parental) TT x tt
T T T t
t geno- all Tt T geno – ¼ TT, ½ Tt, ¼ tt
t pheno – all tall t pheno – ¾ Tall; ¼ short
(3:1 ratio)
Other important monohybrid crosses
T t T t
T geno- ½ TT; ½ Tt t geno – ½ Tt; ½ tt
T pheno – all Tall t pheno– Tall; ½ short
• Dihybrid cross – follows two traits
Tt Tt
Tt Tt
TT Tt
Tt tt
Tt Tt
Tt Tt
Tt tt
Tt Tt
Note 9:3:3:1
phenotypic ratio

14. Complex Genetics
• Incomplete Dominance – intermediate/blended phenotype
Ex) snap dragons  Red (RR) X White (R’R’)  all Pink
R R R R’
R’ geno- all RR’ R’ geno- ¼ RR; ½ RR’; ¼ R’R’
R’ pheno-all pink R’ pheno- ¼red; ½ pink; ¼ white
• Codominance – both parental phenotypes show up in offspring
Ex) Chickens  Black x White Black and White feathers
• Multiple Allelism – trait with 3+ alleles
ex) A, B, O blood types
• Sex Linkage – genes carried on sex chromosomes
Ex) hemophilia, color blindness Cross shows a
carrier female and a normal male. For a female to
inherit the trait the father must have it and the
mother must at least be a carrier
• Polygenic Inheritance – traits controlled
by many genes Ex) Height, hair color
• Aneuploidy – condition caused by
having abnormal chromosome number.
Ex) Down’s Syndrome aka Trisomy 21
RR’ RR’
RR’ RR’
RR RR’
RR’ R’R’

15. DNA Technology
• Today, DNA techniques include:
1) DNA Extraction – the opening of cells to separate/isolate DNA
from other cell parts
2) Cutting DNA – large DNA molecules are cut into smaller fragments
using restriction enzymes. These enzymes recognize and cut DNA
at specific sequences. See Fig 13-5 p322.
3) Separating DNA – DNA fragments can be separated and analyzed
using gel electrophoresis. This process allows scientists to
compare genomes of different organisms, separate genes, and
create DNA “fingerprints”
4) Sequencing DNA – this process allows scientists to determine the
sequence of N-bases in DNA.
5) Recombinant DNA – scientists can cut DNA from two sources with
the same restriction enzyme and combine them. This is used in
genetic engineering. This process has been used to create human
proteins used to treat disease, create pest-resistant crops, and for
many other purposes.
6) Copying DNA – polymerase chain reaction (PCR) has been
developed that makes many copies of a small amount of DNA. See
Fig 13-8 p325.

16. Evolution
• Charles Darwin – proposed that organisms (species or populations)
change over time
• Occurs by Natural Selection – “survival of the fittest”
• Lines of evidence
1) fossils (geologic time)
2) Homologous Structures –
same basic structure formed from
same embryonic tissue
3) Analogous Structures – same
basic functions due to same
environmental pressures
4) Vestigial Structures – structures
that have lost function ex) appendix
5) Embryology – embryos of various
species appear identical
6) Biochemistry – DNA and protein amino acid sequence comparisons
• Adaptive radiation – an ancestral
species radiates or diverges into many
species. Ex) Galapagos Finches
• Origin Ideas
Urey and Miller simulated Earth’s early environment and created
organic compounds like amino acid
Endosymbiont theory – eukaryotic
cells formed when prokaryotic cells began
to live together permanently
Homologous
Vestigial

17. Classification
• Carolus Linnaeus – developed 7 categories of classification
• Also developed binomial nomenclature – naming using the genus and species
names to refer to an organism
• Classification tools include dichotomous keys – a series of paired statements
that lead to the name of an organism
1a) Object has no sides . . . . . . . . . . . . . .Go to 2
1b) Object has sides . . . . . . . . . . . . . . . . . Go to 3
2a) Object is oblong . . . . . . . . . . . . . . . . . oval
2b) Object is symmetrical . . . . . . . . . . . circle
3a) Object has 3 sides . . . . . . . . . . . . . . . triangle
3b) Object has 4 sides . . . . . . . . . . . . . . . Go to 4
4a) Object has 4 equal sides . . . . . . . . . . square
4b) Object doesn’t have 4 equal sides . . rectangle

18. Kingdoms
• http://www.ric.edu/ptiskus/Six_Kingdoms/Index.htm
• How are organism placed into their kingdoms?
1) Cell type, complex or simple
2) Their ability to make food
• 3) The number of cells in their body
• Five Kingdom System
Monera – all prokaryotic includes the bacteria
Protista – most are unicellular, eukaryotic, and aquatic
Fungi – all eukaryotic heterotrophs that act as decomposers
Plantae – all eukaryotic autotrophs
Animalia – all eukaryotic heterotrophs that must eat other
organisms for food
• In Six Kingdom system the Kingdom Monera has been divided into
the Archaebacteria and the Eubacteria
Archaebacteria – are extremists that live in hot, acidic, saline, or
other harsh environments
Eubacteria are “true” bacteria that come in 3 shapes
1) round (coccus)
2) rod (bacillus)
3) spiral (spirillus)

19. Plants
• Plant cell structure – cell walls, large vacuole, chloroplasts
• Photosynthesis
• Classification – 4 groups
1) Nonvascular – no true roots/stems/leaves – ex) mosses (Bryophytes)
2) Seedless vascular plants – Ferns
3) Vascular with seeds in cones – Gymnosperms (pines, fir, spruce)
4) Vascular with seeds in fruits – Angiosperms – flowering plants
• Types of Vascular Tissue
A) Xylem – transports water from roots to leaves
B) Phloem – transports sugars from leaves to roots
Reproductive Life Cycle – called Alternation of Generations
• Tropisms – growth responses to stimuli – often controlled by hormones like
auxins and gibberellins
Phototropism Gravitropism Thigmotropism

20. Animals
• Modes of reproduction
• Animal cell structure – no cell wall, small vacuoles, no plastids,
centrioles
• Classification – 2 main groups
Vertebrates Invertebrates
Phylum Chordata 9 Phyla
3 classes of fish Arthropods – insects (6 legs)
Amphibians (exoskeleton) - arachnids (8 legs)
Reptiles - crustaceans
Aves (birds) Mollusks – have shell created by
Mammals structure called mantel
Human Anatomy – Look through the chapters in your book regarding
anatomy.
* Neurons
* Heart
* Kidneys
Animal Behavior – responses that allow an organism to respond to
stimuli
1) Innate Behavior – instincts, inherited, inborn behaviors
ex) circadian rhythms – daily patterns of activity – including
feeding behaviors - nocturnal
ex) annual rhythms – yearly patterns of activity – including
courtship, estivation, hibernation, migration
2) Learned Behavior – based on experience
ex) imprinting – recognition of parents
ex) Trial and error learning
ex) Conditioning (Pavlov’s dog) – learning by association
3) Social Behavior – division of labor as in a termite or ant colony

21. Biologists
• Robert Hooke – discovered and named the cell with crude
microscope
• Anton van Leeuwenhoek - saw “wee little beasties” living cells for
the first time
• Gregor Mendel – is the father of genetics – discovered the basic
patterns of inheritance in pea plants
• Charles Darwin – is the father of evolution theory – proposed that
organisms that are most fit or best adapted to their environment are
more likely to survive – called Natural Selection
• James Watson and Francis Crick – discovered the double helix
structure of DNA by examining an x-ray made by Rosalind
Franklin and Maurice Wilkins
• Charles Drew – associated with our understanding of the ABO
blood groups and transfusion
• Carolus Linnaeus – binomial nomenclature and classification of
organisms
• Rachel Carson – wrote “Silent Spring” bringing to public attention
the dangers of pesticides particularly DDT – this toxin
bioaccumulates in the bodies of top consumers
• Jane Goodall – studied chimpanzee behavior
• Louis Pasteur – helped disprove abiogenesis or spontaneous
generation by creating a s-neck flask and showing that
microorganisms spoil food