Environment management certificate

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IVAN MOSES OKUNI

From UBC

Environment

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Elemental Analysis of Plants using ICP-OES(2023)

The Hebrew University of Jerusalem

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Having products that are safe, and whose performance has been assessed and conditions of use specified (taking into account progress in knowledge, diversity and any specific aspects of crop situations) is a major issue for farmers, consumers, industry and the environment. The use of plant protection products may cause direct or indirect risks to humans, whether this be the user or the general population, as well as to ecosystems. Assessing pesticides before they can be placed on the market, and monitoring them post-authorisation, are vital to public and environmental health.

Up to 40% of food crops are lost to plant pests and diseases annually.

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River basins appear to be important in the Philippines due to rising water demand for residential needs, agriculture, commerce, and industry. While the country has a total available freshwater resource of 145,900 million Cubic Meters per year based on an 80% probability for surface water and 20,000 million Cubic Meters per year for groundwater recharge or extraction (ASEAN, 2005), the concern for sustainable water supply continues to be a major concern due to the continued degradation of river basins and watersheds.

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The study was designed to evaluate the antibacterial potential of three plants which are Andrographis paniculata, Durio zibethinus and Psidium guajava. Andrographis paniculata leaves (30mg/ml) and roots (30 mg/ml), Durio zibethinus wood bark (10mg/ml), and Psidium guajava leaves (15mg/ml) extract was obtained through the process called maceration, filtration, evaporation and the paste form was freshly reconstitute in dimethyl sulfoxide (DMSO) and tested against Staphylococcus aureus for Andrographis paniculata, Psidium guajava. Streptococcus agalactiae for Durio zibethinus and Psidium guajava and Escherichia coli for Durio zibethinus using Kirby Baur technique and the plates were incubated at 37 ºC. The zone of inhibition was measured after 24 hours and recorded in millimeters. The combination study was conducted using extract in combination with Penicillin G (6.25 µg/ ml) and erythromycin (15 µg/ ml; Andrographis paniculata) with the propotion of 1:1 in homogenous condition and incubated at 37 ºC for 24 hours. The zone of inhibition was measured and recorded. Mean and standard deviation was calculated. Andrographis paniculata do possesses some antibacterial activity against Staphylococcus aureus. Leaves (17.33 mm), roots (10.67 mm), erythromycin (24.00 mm), leaves and erythromycin (20.67 mm), roots and erythromycin (21.67 mm), leaves and roots (17.33 mm). Wood bark against Streptococcus agalactiae (14.67 mm), Penicillin G (14.00 mm), and combination (16.67 mm). Durio zibethinus showed antibacterial activity against Escherichia coli (11.00mm) and Penicillin G (13.33 mm). Psidium guajava leaves extract were having slightly higher activity than Penicillin G and in combination activity, leaves were having a slightly higher activity than Penicillin G.

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Open Access Research Paper

In Israel, desalinated water is a major source of drinking water. Previous studies have suggested that the levels of iodine in water provided by authorities may not accurately reflect the levels reaching end-users. Materials and Methods: We analyzed 21 tap water samples collected from different localities across Israel, 13 posttreated desalinated water samples from three of the largest Israeli desalination plants, and several natural water samples. An improved method of ICP-MS developed in our laboratory was used to analyze the content of iodine and other macro-elements, and determination of iodine was performed in alkaline media. Results: Our results showed that it is possible to distinguish between sample groups based on iodine concentration, water hardness, and Ca/Mg ratio. The median iodine concentrations for four groups of tap water samples ranged from 0.3 to 12.3 μg/L, which is lower than the concentrations previously reported by other researchers in Israel. Based on typical consumption, the water samples can provide no more than 3.39% of the recommended dietary allowance level for iodine. The analysis of post-treated desalinated water samples indicated that these waters comply with industrial specifications but contain only trace concentrations of iodine and much less magnesium than recommended by different public health authorities for public consumption of drinking water. Conclusion: The total iodine concentrations found were lower than several observations reported in previous years in the literature. There are currently no strict regulations regarding iodine and magnesium levels in drinking and/or softened (desalinated) water, but the intensive desalination plant application is already exhibiting a negative impact on public health. Further investigations are needed, but the present study provides useful insights for developing an effective policy to ensure adequate iodine supply for the population of Israel through drinking water.

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