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Ethidium homodimer is responsible for the red color observed. How is.pdf

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kourystephaniamari30

Ethidium homodimer is responsible for the red color observed but cannot cross intact plasma membranes. Calcein is a green dye that can cross membranes but is then trapped inside cells by esterase, which makes it negatively charged. Ethidium homodimer, a positively charged stain, also becomes trapped inside cells with calcein.

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Ethidium homodimer is responsible for the red color observed. How is it able to cross an intact plasma membrane? a. binds to calcein b. it can't c. esterase d. binds to flippase Solution 1. Calcein, is a green membrane permeable flourescent dye that is hydrolyzed by esterase, causing it to become negatively charged and be retained in the cytoplasm, along with ethidium homodimer, a positively charged flourecent nucleic acid stain that cannot cross intact cell membranes.

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Ethidium homodimer is responsible for the red color observed. How is.pdf

  • 1. Ethidium homodimer is responsible for the red color observed. How is it able to cross an intact plasma membrane? a. binds to calcein b. it can't c. esterase d. binds to flippase Solution 1. Calcein, is a green membrane permeable flourescent dye that is hydrolyzed by esterase, causing it to become negatively charged and be retained in the cytoplasm, along with ethidium homodimer, a positively charged flourecent nucleic acid stain that cannot cross intact cell membranes.