The document describes the aseptic technique for transferring inoculum from bacterial cultures using an inoculating loop. It emphasizes the importance of sterilizing the loop by flaming it and performing the transfer within a sterile environment, such as a laminar air flow cabinet, to avoid contamination. Proper steps include waiting for the loop to cool slightly before touching the bacterial culture and transferring it to a new nutrient medium.

1. Using an inoculating loop, demonstrate how to aseptically remove some inoculum from either a
broth tube, slant tube, or petri plate and inoculate a sterile broth tube, slant tube or petri plate
without introducing outside contamination.
Solution
Answer:
1) Before inserting loop into bacterial culture (broth tube, slant tube and petriplate), loop should
be kept in flame until it appears red, after that wait for few seconds before picking a bacterial
culture (as loop with more heat may kill bacterial culture) and touch from one end culture and
transfer into to new nutrient medium.
All this procedure should be done in sterile cabin called as laminar air flow which blows the air
continuosly.