Creative Enzymes has long demonstrated expertise and reliability in development and establishment of biocatalysis systems. We provide products, services, and consultation covering every step in the whole process of a biocatalyst development, including the design, modification, expression, purification, production, and validation of specific enzymatic or microbial systems that catalyze the desired reactions. https://www.creative-enzymes.com/service/Biocatalysis-Services_67.html

1. Biocatalysis
Creative Enzymes Inc.

2. 1 Introductions
Biocatalysis is the use of living systems or their components to accelerate chemical
reactions. In the process of biocatalysis, natural catalysts (such as enzymes)
chemically transform organic compounds. These two enzymes that have been
more or less isolated and those that are still present in living cells are both used.
The process of organic synthesis using natural or modified enzymes is called
chemical enzymatic synthesis. Reactions performed by enzymes are classified as
chemical enzymatic reactions.

3. 2 Asymmetric Biocatalysis
The use of biocatalysis to obtain enantiopure
compounds can be divided into two different
methods:
1.Kinetic resolution of a racemic mixture
2.Biocatalyzed asymmetric synthesis
In kinetic resolution of a racemic mixture, the presence of a chiral
object (the enzyme) converts one of the stereoisomers of the
reactant into its product at a greater reaction rate than for the other
reactant stereoisomer. The stereochemical mixture has now been
transformed into a mixture of two different compounds, making
them separable by normal methodology.

4. 3 Asymmetric Biocatalysis
Biocatalyzed kinetic resolution is utilized extensively in the
purification of racemic mixtures of synthetic amino acids. Many
popular amino acid synthesis routes, such as the Strecker Synthesis,
result in a mixture of R and S enantiomers. This mixture can be
purified by (I) acylating the amine using an anhydride and then (II)
selectively deacylating only the L enantiomer using hog kidney
acylase.

5. 4 Significance of Biocatalysis
The natural, green biological catalysts attract
fast growing attentions for many benefits,
such as sustainability, high efficiency, and little
waste footprint. However, developing and
setting up such systems could be challenging,
given that the nature of these biocatalysts is
distinct from traditional ones. Furthermore,
maintaining a biocatalytic mechanism
performing as a stable and consistent manner
also requires engineering design and
development on the whole system, in
addition to the molecular biology and
biochemistry techniques that are essential to
start feasibility evaluation on the biological
catalysts.

6. 5 Our Services
Creative Enzymes presents the professional
development platform for biocatalysts by
integrating the expertise on molecular biology
and protein engineering, and experience on
development of industrial processes. Creative
Enzymes has extensive partnership with
academic institutes working at the cutting
edge technologies of biocatalysis. This enables
Creative Enzymes to provide the latest
solutions and unbiased selection of the best
products for our clients.

7. 6 Catalytic Assays
Catalytic assays are performed to
determine the amount of the
enzyme or substrate in a sample
quantitatively. Also, it can be used
to prove presence or absence of
the enzyme in a distinct specimen,
like an organism or a tissue, as a
qualitative approach.
Creative Enzymes offers the
service of catalytic assays for the
best evaluation of target
biocatalysts. We have extensive
experience with various types of
biocatalysts and therefore offer
professional one-stop services:
•Reaction condition optimization.
•Catalytic assay development and
validation.
•High-throughput strategies design.
•Assay evaluation.

8. 7 Computational Modeling
In the process of identification and
design of a biocatalyst, experience and
skills are often required along with
tedious cycles of design-trial-
optimization. Computational modeling
technique is an alternative way from
traditional experimental methods to
study bioactive molecules for target
biocatalysts.
Creative Enzymes’ service of
computational modeling helps customer
find the most suitable substrates for their
target. With variety of in silico tools, the
professional services provide much
insight into the desired biocatalysts at a
relatively low cost:
•Chemical database search.
•Three-dimensional structure prediction
and determination.
•Virtual screening of substrates.
•Rational design of the biocatalyst.

9. 8 Substrate Profiling
Substrate selectivity or
specificity is the key to
successful development of
biocatalysts. As a natural
catalyst, enzyme inherently
presents promiscuity over similar
molecules. Therefore,
understanding substrate
specificity of a particular enzyme
holds an important place in
Biocatalysis development.
Creative Enzymes offers the service of
substrate profiling, aimed at evaluation of
substrate specification of a known bio-
catalysis reaction. Enabled by our best
knowledge of bioinformatic technique, we
offer professional one-stop services:
 Substrate assay design and validation.
 Synthetic peptide library construction.
 MSP-MS assay.
 Data analysis and development planning

10. 9 Other Services

11. 10 Our Features
• Biodegradable and
environmental friendly reagents
and solvents
• Services and consultation tailored
to specific development needs
• Efficient synthetic and processing
routes
• Decreased cost and turnaround time
through minimization of side
reactions, by-products and
impurities
• Optimization and enhancement of
conversion rates, yields, and
selectivity
• Design and setup of self-
regenerating or low-maintenance
systems

12. Address: 45-1 Ramsey Road, Shirley, NY
11967, USA
Email: info@creative-enzymes.com
Fax: 1-631-938-8127
Tel: 1-631-562-8517
1-516-512-3133
Get In Touch

13. THANKS!