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Innovation in Fruits and Vegetables
And a little bit on Communicating the Science of Science
Communication
Kevin M. Folta
Professor and Chair
Horticultural Sciences Department
kfolta.blogspot.com
@kevinfolta
kfolta@ufl.edu
"There is a path to truth and sincerity that you must
guard and defend“
-- Teruyuki Okazaki
All due to mutations and genomic
alterations
All required human intervention for
breeding and/or selection
Technology Exists NOW
Research has been published demonstrating that
transgenic techniques can:
Biofortify foods with vitamins, minerals, phytonutrients
Grow plants in marginal areas
Grow plants with fewer inputs
Efficient use of fertilizers
Insect resistance
Disease resistance
What fruits and veggies are available now?
Major Themes
Silencing
Overexpression
Cisgenesis
Gene Silencing
What else could we silence?
Gene Silencing
What else could we silence?
- allergens
- anti-nutrients
- physiology associated with post-harvest decay
- many others!!
RNAi as a means to silence genes
Use in transgenics follows its
natural intent
Squash
Papaya
Plum
Others
Systemic protection
New Awareness
Farmers
Developing World
The Needy
Food Safety
Environment
Consumers
New Awareness
Farmers
Developing World
The Needy
Food Safety
Environment
Consumers
Flavr Savr Tomato
First commercialized GE
fruit or vegetable
Suppression of
aminoglycoside 3'-phosphotransferase II
A polygalacturonase
Idea was to slow ripening process
 Asgrow Seed Company’s “Freedom II” is the first variety of genetically
engineered squash and the second GM crop to be cleared by US
regulators. Many squash varieties sold now have viral coat proteins
expressed via a transgene.
Squash
Watermelon Mosaic Virus
Mosaic and rugosity of foliage and color breaking on fruit of
straightneck yellow squash caused by Watermelon Mosaic
Potyvirus (formerly WMV-2).
Summer squash
leaves show mosaic
symptoms caused
by Watermelon
Mosaic Potyvirus
(formerly WMV-2).
APHID-TRANSMITTED VIRAL DISEASES ARE MAJOR PROBLEMS.
Zucchini Yellow Mosaic Virus
Zucchini Yellow
Mosaic Potyvirus
produces prominent
yellow mosaic and
distortion of foliage
and greatly malformed
fruit as shown here.
APHID-TRANSMITTED VIRAL
DISEASES ARE MAJOR PROBLEMS.
Cucumber Mosaic Virus
Plum
Pox
Virus
Plum Pox Virus
Infects rosaceous stonefruits.
Aphid transmitted
Single-stranded RNA virus
Some natural resistance
Engineered into plums by Ralph
Scorza et al., USDA
Large numbers of small interfering
RNAs suggest a potentially potent
response.
Deregulated, process took about 20 years. Available if needed.
Papaya Ringspot Virus
1992- PSRV cut production from 55M
pounds to 26 M pounds
1998-- PSRV resistant introduced
2001 – 40 M pounds
Papaya Ringspot Virus
Good example of RNAi suppression
Why Virus-resistant papaya is such a victory
-- Done by the public sector
-- IP rights transferred to local papaya industries
-- Japan eventually allows GM papaya
-- non-GM surrounded by GM to protect them
Downside
Gene flow
Non Browning Apples
Silencing a gene that leads to discoloration
X
X
Farmers
Consumers
Environment
Needy
Only 4 fruits.
Why?
In Development!!
Golden Bananas Beta carotene producing
X
Farmers
Consumers
Environment
Needy
Allergy-Free Peanuts
Peanut – RNAi suppression Ara h2 X
Farmers
Consumers
Environment
Needy
Allergy Free Wheat
Using RNAi to repress gliadin levels
BS2 Tomato
A pepper gene in tomato eases black spot and wilt.
X
X Farmers
Consumers
Environment
Needy
BS2 Tomato
A pepper
gene in
tomato eases
black spot
and wilt.
High Anthocyanin Tomato
A transcription factor excites anthocyanin production in fruits
X
X Farmers
Consumers
Environment
Needy
X
Longer shelf life too.
Grapes resistant to Pierce’s Disease
X
X
Farmers
Consumers
Environment
Needy
X
Stopping Citrus Greening
NPR1 -- Regulator of plant defense response.
Shown in many systems to confer broad-spectrum tolerance to pathogens..
Stopping Citrus Greening
Spinach defensin
Lytic peptides
Many show
promise
Earliest
deregulation is
2019
Bacterial Wilt in Bananas
>70% of carbohydrate calories for
some areas
GM trials in Uganda
X
X
Farmers
Consumers
Environment
Needy
X
Over expression of rice
Xa21 gene
Tripathi et al, 2014
Overexpression of Ferredoxin like protein
But What About Agricultural Biotechnology?
Generally:
People don’t have any idea what it is.
People don’t know how biology works.
Few understand farming and supply chains.
The just know that they don’t like biotech crops.
Relativenumberinpopulation
Relative scientific understanding
Activists
Farmers,
scientists,
Etc.
MOST PEOPLE!!!!
Based on findings from UF PIE Center
Avoid these Mistakes
Avoid “feed the world” rhetoric
Discuss strengths and limitations
Not a panacea, not a disaster
Never get backed into the “science no”
“Can you guarantee that these are absolutely safe?”
Watch the News, Engage the Comments, Create the Contrast
Use your real name
Provide an email address
Offer to help interpret the media
Always be as kind as possible
How do we participate effectively?
Winning the Emotional Capital
Consequences and Lost Opportunities
Opposition to this technology has significant costs.
The needy
The environment
Farmers
Consumers
Technology Exists NOW
Research has been published demonstrating that
transgenic techniques can:
Help farmers.
Biofortify foods with vitamins, minerals, phytonutrients
Grow plants in marginal areas
Grow plants with fewer inputs
Efficient use of fertilizers
Insect resistance
Disease resistance
Technology Exists NOW
Research has been published demonstrating that
transgenic techniques can:
Biofortify foods with vitamins, minerals, phytonutrients
Grow plants in marginal areas
Grow plants with fewer inputs
Efficient use of fertilizers
Insect resistance
Disease resistance
Conclusions:
The pipeline started with farm-centric products
Few horticultural crops are commercialized due to cost
and high barriers in deregulation.
The future products emphasize traits with direct
consumer benefit
Emphasizing benefits for consumers, the environment,
the developing world and the farmer helps to change
hearts and minds.
Other countries will independently pursue the technology.

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Innovation in Fruits and Vegetables

  • 1. Innovation in Fruits and Vegetables And a little bit on Communicating the Science of Science Communication Kevin M. Folta Professor and Chair Horticultural Sciences Department kfolta.blogspot.com @kevinfolta kfolta@ufl.edu
  • 2. "There is a path to truth and sincerity that you must guard and defend“ -- Teruyuki Okazaki
  • 3. All due to mutations and genomic alterations All required human intervention for breeding and/or selection
  • 4.
  • 5. Technology Exists NOW Research has been published demonstrating that transgenic techniques can: Biofortify foods with vitamins, minerals, phytonutrients Grow plants in marginal areas Grow plants with fewer inputs Efficient use of fertilizers Insect resistance Disease resistance
  • 6. What fruits and veggies are available now?
  • 8. Gene Silencing What else could we silence?
  • 9. Gene Silencing What else could we silence? - allergens - anti-nutrients - physiology associated with post-harvest decay - many others!!
  • 10. RNAi as a means to silence genes Use in transgenics follows its natural intent Squash Papaya Plum Others
  • 12. New Awareness Farmers Developing World The Needy Food Safety Environment Consumers
  • 13. New Awareness Farmers Developing World The Needy Food Safety Environment Consumers
  • 14. Flavr Savr Tomato First commercialized GE fruit or vegetable Suppression of aminoglycoside 3'-phosphotransferase II A polygalacturonase Idea was to slow ripening process
  • 15.  Asgrow Seed Company’s “Freedom II” is the first variety of genetically engineered squash and the second GM crop to be cleared by US regulators. Many squash varieties sold now have viral coat proteins expressed via a transgene. Squash
  • 16. Watermelon Mosaic Virus Mosaic and rugosity of foliage and color breaking on fruit of straightneck yellow squash caused by Watermelon Mosaic Potyvirus (formerly WMV-2). Summer squash leaves show mosaic symptoms caused by Watermelon Mosaic Potyvirus (formerly WMV-2). APHID-TRANSMITTED VIRAL DISEASES ARE MAJOR PROBLEMS.
  • 17. Zucchini Yellow Mosaic Virus Zucchini Yellow Mosaic Potyvirus produces prominent yellow mosaic and distortion of foliage and greatly malformed fruit as shown here. APHID-TRANSMITTED VIRAL DISEASES ARE MAJOR PROBLEMS.
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  • 23. Plum Pox Virus Infects rosaceous stonefruits. Aphid transmitted Single-stranded RNA virus Some natural resistance Engineered into plums by Ralph Scorza et al., USDA
  • 24.
  • 25. Large numbers of small interfering RNAs suggest a potentially potent response.
  • 26. Deregulated, process took about 20 years. Available if needed.
  • 27. Papaya Ringspot Virus 1992- PSRV cut production from 55M pounds to 26 M pounds 1998-- PSRV resistant introduced 2001 – 40 M pounds
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  • 29. Papaya Ringspot Virus Good example of RNAi suppression
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  • 33. Why Virus-resistant papaya is such a victory -- Done by the public sector -- IP rights transferred to local papaya industries -- Japan eventually allows GM papaya -- non-GM surrounded by GM to protect them Downside Gene flow
  • 34. Non Browning Apples Silencing a gene that leads to discoloration X X Farmers Consumers Environment Needy
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  • 39. Golden Bananas Beta carotene producing X Farmers Consumers Environment Needy
  • 40. Allergy-Free Peanuts Peanut – RNAi suppression Ara h2 X Farmers Consumers Environment Needy
  • 41. Allergy Free Wheat Using RNAi to repress gliadin levels
  • 42. BS2 Tomato A pepper gene in tomato eases black spot and wilt. X X Farmers Consumers Environment Needy
  • 43. BS2 Tomato A pepper gene in tomato eases black spot and wilt.
  • 44.
  • 45. High Anthocyanin Tomato A transcription factor excites anthocyanin production in fruits X X Farmers Consumers Environment Needy X Longer shelf life too.
  • 46. Grapes resistant to Pierce’s Disease X X Farmers Consumers Environment Needy X
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  • 49.
  • 50. NPR1 -- Regulator of plant defense response. Shown in many systems to confer broad-spectrum tolerance to pathogens..
  • 51.
  • 52. Stopping Citrus Greening Spinach defensin Lytic peptides Many show promise Earliest deregulation is 2019
  • 53. Bacterial Wilt in Bananas >70% of carbohydrate calories for some areas GM trials in Uganda X X Farmers Consumers Environment Needy X
  • 54. Over expression of rice Xa21 gene Tripathi et al, 2014
  • 56.
  • 57. But What About Agricultural Biotechnology? Generally: People don’t have any idea what it is. People don’t know how biology works. Few understand farming and supply chains. The just know that they don’t like biotech crops.
  • 59. Avoid these Mistakes Avoid “feed the world” rhetoric Discuss strengths and limitations Not a panacea, not a disaster Never get backed into the “science no” “Can you guarantee that these are absolutely safe?”
  • 60. Watch the News, Engage the Comments, Create the Contrast Use your real name Provide an email address Offer to help interpret the media Always be as kind as possible
  • 61. How do we participate effectively? Winning the Emotional Capital Consequences and Lost Opportunities Opposition to this technology has significant costs. The needy The environment Farmers Consumers
  • 62. Technology Exists NOW Research has been published demonstrating that transgenic techniques can: Help farmers. Biofortify foods with vitamins, minerals, phytonutrients Grow plants in marginal areas Grow plants with fewer inputs Efficient use of fertilizers Insect resistance Disease resistance
  • 63. Technology Exists NOW Research has been published demonstrating that transgenic techniques can: Biofortify foods with vitamins, minerals, phytonutrients Grow plants in marginal areas Grow plants with fewer inputs Efficient use of fertilizers Insect resistance Disease resistance
  • 64. Conclusions: The pipeline started with farm-centric products Few horticultural crops are commercialized due to cost and high barriers in deregulation. The future products emphasize traits with direct consumer benefit Emphasizing benefits for consumers, the environment, the developing world and the farmer helps to change hearts and minds. Other countries will independently pursue the technology.