dendritic cells in cancer

1. SEMINAR ON DENDRITIC CELL
Presented by :-
Javed Ali Khan
M.Pharm(p’cology)
AIP
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2. DENDRITIC CELLS(DC)
What is dendritic cells ?
 Paul langerhans 1st described DC in human skin in 1868
 In 1973 for Ralph Steinman and Zanvil Cohn to begin
the modern era of dendritic cell science by showing
that dendritic cells are a new class of white blood cells
with a number of distinctive features and functions.
 2011 Nobel Prize in Physiology-Medicine awarded to
Bruce A. Beutler and Jules A. Hoffmann (for their
discoveries in innate immune recognition) and Ralph
M. Steinman (for his discovery of the DC).
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3. Dendritic cells (DCs) are immune cells that
form part of the mammalian immune system.
Their main function is to process antigen
material and present it on the surface to other
cells of the immune system, thus functioning
as antigen-presenting cells. They act as
messengers between the innate and adaptive
immunity.
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4. DCs are Bone-Marrow (BM)-derived
leukocytes & are the most potent type of APC.
DC are mononuclear phagocytic cells which
uptake antigen by endocytosis & then present
peptide in their MHC class II molecules to T-
cells.
DC are potent APC & are key cells in the
adaptive immune system.
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5. DC have 2 pathways
The myeloid pathway(non-lymphoid) the lymphoid pathway which
generate plasmocytoid DCs
Langerhans cells found interstetial DCs found in all other tissue
in stratified epithelial
i.e skin
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6. • Dc can be isolate from-
Spleen,blood,skin,lungs (mouse)
lymph node,liver,lungs (rat)
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7. • DC activation & maturation-
molecule which activate DCs are CD40,
TNF-R,IL-IR & trigger their transition from
immature DC to mature DC
DC precursor------------>imDC------------->mDC
GM-CSF+IL-4
Maturation -
stimulus
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8. Life span of dendritic cells
 Activated macrophages have a lifespan of only a few
days
 The lifespan of activated dendritic cells,varying
according to type and origin
 Immature dendritic cells seem to be able to exist in an
inactivated state for much longer.
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9. DC markers
For mouse---- 33D1, CD80(B7-1), CD83,
CD86(B-7), CD207(langerhans)
For humans-----CCR7(CD 197), CD1a, CD83,
CD86(B7-2)
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10. Research challenges
Dendritic cell plays vital role in---
 oncology
 HIV/AIDS
 auto immunity
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11. • DC in oncology and use as autologous thearpy
DC cell therapy is an immunological treatment
of cancer
Example-----
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12. Patients peripheral blood mononuclear cells are taken
They are cultured into DC
Then DC are cultured for 8 days
During culturing process we introduce
an antigen to make them specific for a
patient of cancer, this antigen is derived
from patients own tissue
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13. Then 1 million cells (DC) in 6 vials
These infusions are given at 20
days interval
Observe that immune enhancement of T-cells against cancer
Stoppage of progression of cancer
results
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15. Thank you
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