High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

1. High performance liquid chromatography(HPLC)
Presented by:
Md. Faysal Ahmed
Id : 151-29-760

2. Introduction:
HPLC stands for “High-performance liquid chromatography”(sometimes referred to
as High-pressure liquid chromatography).
It is used in biochemistry and analytical chemistry to identify, quantify and purify
the individual components of a mixture.

3. Principle of HPLC:
High performance liquid chromatography (HPLC) is basically a highly improved form of
column liquid chromatography to separate compounds that are dissolved in solution.
High performance liquid chromatography (HPLC) is principle is based on adsorption as
well as partition chromatography is depending on the nature of stationary phase.
HPLC is a chromatographic technique that can separate a mixture of compounds.

5. Applications of HPLC
 Pharmaceutical field
 Chemical & Petrochemical industry
 Forensic
 Biochemical separations
 Food analysis
Qualitative analysis : Checking the purity of a compound
Quantitative Analysis:
Direct comparison method
Calibration curve method

6. Advantages of HPLC
 High sensitivity
 High performance
 Rapid process and hence time saving
 Can be used for qualitative as well as quantitative estimation.
 Separations fast and efficient (high resolution power)
 Accurate quantitative measurements.