1. KOCHAPHAN
BOWONCHAIRIT
520501217
&
KANJANA
JEKSOMBAT
530510221

5. FUNCTION AS
BARRIER TO ESTABLISH DISTINCT
FLUID COMPARTMENTS
TO MAINTAIN HOMEOSTASIS

6. TRANSPORTATION ACROSS
CELLULAR SHEETS HAS
TWO DISTINCT PATHWAY
TRANSCELLULAR
PARACELLULAR

8. • Claudins Constitute Paired TJ
TOPIC I Strands
• The Claudins Family, A Newly
TOPIC II Emerging Gene family
• Claudins Are Functionally Involved in
TOPIC III the TJ Barrier
• Aqueous Pores within TJ Strands
TOPIC IV

9. CLAUDINS
CONSTITUTE
PAIRED
TJ STRANDS

13. OCCLUDINS

16. 2 NOVEL
INTEGRAL MEMBRANES PROTEINS
WITH 4 TRANSMEMBRANES DOMAIN

17. COPARTITION WITH OCCLUDIN

18. THEY RECONSTITUTED TJs
WITH FIBROBLAST

20. ANOTHER PROTEINS LOCALIZED ON
TJs

21. NO ABILITY TO RECONSTITUTED
TJs WITH FIBROBLAST

22. THE MOLECULE OF
TJ STRNADS IS
MORE COMPLEX
THAN EXPECTED

23. THE
CLAUIN
FAMILY
A NEWLY EMERGING
GENE FAMILY

26. OSP
RVP1
TMVCF
CPE-R

27. CLUADIN-11
CLUADIN-3
CLUADIN-5
CLUADIN-4

28. CLAUDIN-3
mRNA CLAUDIN-4,
-7 AND -8
CLAUDIN-1
CLAUDIN-2

29. MORE THAN 2 SPECIES
OF CLAUDINS ARE
CO-EXPRESSED
IN SINGLE CELL

30. CLAUDIN-
5/TMVCF CLAUDIN-
11/OSP

31. CLAUDINS ARE
FUNCTIONALLY
INVOLVED IN THE TJs BARRIER

33. REPO

34. CLAUDIN-4 WAS IDENTIFIED
AS CPE RECEPTOR
CPE-R
COOH-TERMINAL : C-CPE
SPECIALLY BOUND TO
PORTED
CLAUDIN-4/CPE-R
WHEN C-CPE WAS APPLIED
TOCULTURED
EPITHELIAL CELLS

35. CLAUDIN-4 WAS REMOVED FROM TJs
WITH
TRANSEPITHELIAL
ELECTRICAL
RESISTANCE
AND
THE NUMBER OF TJs
DECREASE

36. CLAUDINS
INVOLVE WITH
THE BARRIER
FUNCTIONS
OF
TJs

37. CLAUDINS
CONSTITUTE
THE WALL
BETWEEN
ENDOTHELIAL
AND
EPITHELIAL CELLS

38. FOR
SEALING
PARACELLULAR
PATHWAY

39. HOWEVER
THIS WALL
CONTAINS
PORES

45. WITHIN TJ STRAN

46. ANALYSIS
OF
HEREDITY
OF
HYPOMAGNESEMIA
NDS

48. CLAUDIN-16/PARACELLIN-1
is directly involved in
A SELECTIVE PARACELLULAR
CONDUCTANCE FOR Mg2+ IONS
of claudin-based strands
OTHER CLAUDINS
CANNOT FORM
THE PORES ON
THE CLAUDIN-BASED
WALL

49. ANALYSIS OF
HETEROGENOUS
SPECIES
WITHIN AND BETWEEN TJ STRANDS
PROVIDE THAT
THE CLAUDIN CAN
CONSTITUTE THE
PORES IN THE WALL

50. WHEN TWO OF
CLAUDINS WERE
COEXPRESSED IN
L FIBROBLASTS,
CLAUDINS WERE
COPOLYMERIZED
INTO INDIVIDUAL
TJs

52. when TWO of
L TRANSFECTANTS
SINGLY EXPRESSING
OCCLUDINS -1, -2 OR -3
were COCULTURED
claudins-3 based
homopolymers
ASSOCIATED WITH
claudins-1 and claudins-
2 base strands

53. But heterotypic paired
strands were NOT
FORMED between
claudins-1- and
claudins-2-based strands

55. when L TRANS
FECTANTS SINGLY
EXPRESSING
OCCLUDINS -1, -2 OR -3
were COCULTURED
with those coexpressing
claudin-1 and -2

56. Claudin-1 homopolymers
latterally associated
with claudin-1/2
heteropolymers to form
paired strands

59. TJ STRAND DIFFER IN
PERMENANABILITY
DEPENDING ON CELL TYPE

60. IT IS NOT CLEAR HOW CLAUDINS AND
OCCLAUDIN ARE ARRANGED TO FORM
INDIVIDUAL TJ STRANDS