Plasma protein

1. dr.msathish@gmail 1
Plasma proteins in health and diseases
Dr. M .Sathishbabu MD
Asst. Professor
Dept of Biochemistry
MGMCRI

2. 2
Why you have know?
• Wilson's disease- Ceruloplasmin
• Nephrotic syndrome - Albumin
• ANAEMIA D.D Se Ferritin levels
• Emphysema - Alpha-1 antitrypsin
• CRP levels – Inflammation

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Answer to this ?
Blood ?
Plasma ?
Serum ?
Plasma protein separation Methods ?

4. Name the plasma proteins and its
function ?
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5. Where it is synthesized ?
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IMMUNOGLOBINS ARE SYNTHESISED BY ?
Mention the function of plasma
proteins ?

6. What are APR
(Acute Phase Reactant )?
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POSITIVE APR?
•AAT
•Hp
•Ceruloplasmin
•C4,C3
•Procalcitonin
•Se AMYLOID A
NEGATIVE APR?
•Transferrin
•Alb
•Transthyretin

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How Albumin is important?
• Levels is increased in ?
• Levels is decreased in ?

8. WHAT IS GLYCATED ALBUMIN ?
• T1/2=15 TO 19 DAYS
• HbA1C
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9. Proteinuria types ?
• Which test will be performed in urine?
• Monochromatic methods overestimate
albumin in fibrinogen and heparin
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Alpha fraction
A-1 Antitrypsin? A-2 Antitrypsin?

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Á1 - AT Deficiency
Emphysema
• Smoking , genetic , loss trou urine
• Elastase Vs Anti elastase
• Functions of Á1 – AT :
• Blocks enzyme action
• Enzymes are present in WBC ,
endothelium, bronchioles. (Elastase,
Chymotrypsin etc)

12. • Elastase Vs A1- antitrypsin
deficiency
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Tissue
damage

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Wilsons disease – Cu toxicity
• What are the functions of ceruloplasmin?
• Kayser Fleischer Ring
• Hepato lenticular degeneration
• Lab finding in Wilsons disease ?

14. K.F RING

15. CERULOPLASMIN
• plasma protein(acute phase reactant)
• alpha2 globulin
• has oxidase activity
• binds 6 atoms cu
• 90% carried by c .p
• normal serum level 20 to 40mg/dl
• <20= disease

17. CMT1(copper
membrane Transporter
INTESTINE
ATOX Metalochaperones
ATP7A NON HEPATIC INTRA
CELLULAR
TRANSPORTER -
MENKES
ATP78 INTRA CELLULAR
HEPATIC
TRANSPORTER-
WILSONS.
HURR

19. Wilson s

20. Wilson s Disease
Hepato lenticular degeneration

21. MENKE KINKY HAIR SYNDROME

22. MENKE KINKY DISEASE
• Even dietary cu is absorbed but cannot
transported vascular space
• Intracellular ATPase defect vs Wilsons hepatic
ATPase
• Kinky hair
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Anaemia –and plasma protein
• Ferritin : storage form
• Apoferritin: (Tf) without iron
• Transferrin: (Tf) transfer form
• CDT: Detection Of Alchol Abuse
Tf

24. Name the proteins that bind hemoglobin and heme in the
circulation.

25. Which is the sensitive indicator of
iron deficiency?
Serum Iron Transferrin Ferritin
Iron Deficiency
ACD

26. A1-Acid glycoprotein:
• Indicator clinical activity of ulcerative colitis
• Increased in inflammation
A1- Fetoprotein :
• Fetal life – normally produced
• Yolksac tumor
• Testicular tumor
• Down syndrome
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27. HAPTOGLOBIN
• Binds extracorpuscular Hb
Hb
Tubular
damage
kidney
Fe loss
Hb + Hp
Excreated without any
damage
-
-

28. • CONSERVATION OF IRON IN THE BODY:
RBC lysis
Hb  Hb-Haptoglobin complex taken up by
liver kupffer’s cells  Iron re- utilized
Globin removed
Heme  Heme-hemopexin complex taken by
Hepatocytes  Iron re-utilized
Porphyrin removed  Bilirubin
Iron  Iron-Transferrin  Storage  Iron re- utilized

29. Name some protease inhibitors ?
• AAT : Cathepsin G,
• AMG
• A-Antichymotrypsin
• A-Antithrombin III --- Inhibit thrombin
thromboembolism
• A-Antiplasmin : Increase clot lysis (hage)
• C1 Inhibitors--- Hereditory angioedema
• Inter-A- Trypsin inhibitor
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30. A2 Macroglobin
• Large glycoprotein
• Neutralize many proteases & cytokines (PDGF)
• Sources: Monocyte, Hepatocyte, Astrocyte
• Functions:
Zn carrier
- of enzyme kinin &Coagulation cascade .
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31. • Estrogen affect levels increased ɣ with
estrogen
• Child bearing age higher levels
• Age : Facilitate cell receptor binding and
uptake
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