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Describe: gene transfer b.stev
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],ALL  genetic material  contained is transferred to the  daughter  cell (Barnes S. N, Curtis H, 1989) Binary fission
cell wall chromosome cell membrane theta replication growth of cell NEXT REPLICATION
new cell wall forms REPLICATED   bacterial   cells (two identical   daughter  cells)
This type of replication is known as: THETA REPLICATION a bi-directional motion  DNA helix unwinds/  replicates / rewinds
cell F- cell F+ bacterial chromosome F plasmid  bacteria join: pili transfer of  F plasmid cell F+ transfer provides:   COMPLEMENTARY DNA CONJUGATION
PLASMIDS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],plasmid   moves to bacterium via the use of an  extension  made by host [ carrier ] cell: - this extension is called:  pili   (Barnes S. N, Curtis H, 1989)
[object Object],[object Object],[object Object],[object Object],[object Object],Transformation NOTE:   not significant in contribution to the dispersal of antibiotic resistant genes
Transduction
SPECIALISED> prophage   – the  altered bacterial DNA performs BINARY FISSION that  creates replicates though the replicates differ: ONE  may produce  virus cells OTHER  creates  defective virus cells   that seek to perform the process of:   generalised transduction  (Pommerville J.C, 2004)
Insertion Sequences small segments DNA: about 1000bp ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Transposons larger   insertion   sequences  that have the  ability:   carry information such as  antibiotic resistance
C C C T A A  T T A G G G G G G A T T  A A T C C C inverted sequence   of   repetitive bases: ability  to move/ insert & disembark  coded sequence of information TRANSPOSON
Genetic Transfer   &   dissemination of drug resistance Transfer,   causes increase   in bacterial population that   have   the   ability  to   resist  specific drugs:  ,[object Object],[object Object],[object Object],[object Object],[object Object]
OVERUSE & MISUSE ENCOURAGES RESISTANT FORMS: 1968-71 >   Guatemala ; 12,000   people died to a resistant form of:  S.dysenteriae ANTIBIOTICS  causes the  sensitive  cells to die,  stimulates   resistant  cells:   grow & to thrive increasing numbers HOSPITALS:   care  taken to prevent  resistant  strains overtaking the  susceptible  strains especially: E.Coli P.Aeruginosa S.Marcesens Proteus (Pommerville J.C, 2004)
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Bibliography Campbell N. A, Reece J. B. (2005).  Biology (7th ed.) . San Francisco:  Benjamin Cummings Pommerville J.C. (2004).  Alcamo’s – Fundamentals of Microbiology   (7 th  ed.). Massachusetts: Jones and Bartlett. Barnes S. N, Curtis H. (1989).  Biology (5th ed.) . New York: Worth

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Gene transfer methods in bacteria

  • 2.
  • 3.
  • 4. cell wall chromosome cell membrane theta replication growth of cell NEXT REPLICATION
  • 5. new cell wall forms REPLICATED bacterial cells (two identical daughter cells)
  • 6. This type of replication is known as: THETA REPLICATION a bi-directional motion DNA helix unwinds/ replicates / rewinds
  • 7. cell F- cell F+ bacterial chromosome F plasmid bacteria join: pili transfer of F plasmid cell F+ transfer provides: COMPLEMENTARY DNA CONJUGATION
  • 8.
  • 9.
  • 11. SPECIALISED> prophage – the altered bacterial DNA performs BINARY FISSION that creates replicates though the replicates differ: ONE may produce virus cells OTHER creates defective virus cells that seek to perform the process of: generalised transduction (Pommerville J.C, 2004)
  • 12.
  • 13.
  • 14. C C C T A A T T A G G G G G G A T T A A T C C C inverted sequence of repetitive bases: ability to move/ insert & disembark coded sequence of information TRANSPOSON
  • 15.
  • 16. OVERUSE & MISUSE ENCOURAGES RESISTANT FORMS: 1968-71 > Guatemala ; 12,000 people died to a resistant form of: S.dysenteriae ANTIBIOTICS causes the sensitive cells to die, stimulates resistant cells: grow & to thrive increasing numbers HOSPITALS: care taken to prevent resistant strains overtaking the susceptible strains especially: E.Coli P.Aeruginosa S.Marcesens Proteus (Pommerville J.C, 2004)
  • 17.
  • 18. Bibliography Campbell N. A, Reece J. B. (2005). Biology (7th ed.) . San Francisco: Benjamin Cummings Pommerville J.C. (2004). Alcamo’s – Fundamentals of Microbiology (7 th ed.). Massachusetts: Jones and Bartlett. Barnes S. N, Curtis H. (1989). Biology (5th ed.) . New York: Worth