What is the temperature requited to denature myoglobin without guanidine?What is delta S and delta H of myoglobin denaturation without guanidine? Solution he temperature to denature myoglobin without guanidine is 30 degree C(celsius). Go= HoTSo = RT ln KD ->(1) Ln KD =(HoTSo)/RT ->2 lnKD=Ho/RT+ So/R( 3) dlnKD/d(1/T)=Ho/R=H0vHoff/R(4) or dlnKD/dT=H0vHoff/RT2(5).