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What is the temperature requited to denature myoglobin without guanidine?What is delta S and
delta H of myoglobin denaturation without guanidine?
Solution
he temperature to denature myoglobin without guanidine is 30 degree C(celsius).
Go= HoTSo = RT ln KD ->(1)
Ln KD =(HoTSo)/RT ->2
lnKD=Ho/RT+ So/R( 3)
dlnKD/d(1/T)=Ho/R=H0vHoff/R(4)
or
dlnKD/dT=H0vHoff/RT2(5)

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What is the temperature requited to denature myoglobin without guani.pdf

  • 1. What is the temperature requited to denature myoglobin without guanidine?What is delta S and delta H of myoglobin denaturation without guanidine? Solution he temperature to denature myoglobin without guanidine is 30 degree C(celsius). Go= HoTSo = RT ln KD ->(1) Ln KD =(HoTSo)/RT ->2 lnKD=Ho/RT+ So/R( 3) dlnKD/d(1/T)=Ho/R=H0vHoff/R(4) or dlnKD/dT=H0vHoff/RT2(5)