A variety of media and culture conditions can be used to culture and identify Listeria monocytogenes. For each, characterize it as selective or differential. Solution Listeria monocytogenes is basically a soil-borne micro-organism. It has been found in various sources such as water, agricultural products, and in animals. Listeria monocytogenes has been known as etiologic agent of listeriosis, causing meningitis, encephalitis, septicemia and endocarditis in humans. various selective and differential methods were developed to isolate the Listeria monocytogenes species as follows Selective Incubation at 30 degree celsius Adding antibiotics such as nalidixic acid Adding high concentarion of salts such as lithium chloride and sodium chloride Adding antifungals such as amphotericin B Differential Adding pH indicator to detect ferementaion of rhamanose to acid Detecting beta heamolysis.

1. A variety of media and culture conditions can be used to culture and identify Listeria
monocytogenes. For each, characterize it as selective or differential.
Solution
Listeria monocytogenes is basically a soil-borne micro-organism. It has been found in various
sources such as water, agricultural products, and in animals. Listeria monocytogenes has been
known as etiologic agent of listeriosis, causing meningitis, encephalitis, septicemia and
endocarditis in humans.
various selective and differential methods were developed to isolate the Listeria monocytogenes
species as follows
Selective
Incubation at 30 degree celsius
Adding antibiotics such as nalidixic acid
Adding high concentarion of salts such as lithium chloride and sodium chloride
Adding antifungals such as amphotericin B
Differential
Adding pH indicator to detect ferementaion of rhamanose to acid
Detecting beta heamolysis