PROTOPLAST ISOLATION FUSION AND REGENERATION TISSUE CULTURE.

8. Non- Ionic Ionic
Most commonly used are soluble
carbohydrates such as mannitol, sorbitol,
glucose,
fructose, galactose and sucrose.
Mannitol, being metabolically inert, is most.
frequently used.
Potassium chloride, calcium chloride
and magnesium phosphate are the
ionic substances in use to maintain
osmotic pressure.
KH2PO4 27.2mg
KNO3 101mg
CaCl2.H2O 1480mg
MgSO4.7H2O 246mg
KI 0.16mg
CuSO4.5H2O 0.025mg
Made to 1 litre with water pH 5.8

10. Protoplast
fusion
Induced
fusion
Chemical
fusion
Chemical
fusion
Spontaneous
fusion