Q47 In DNA replication what is the difference between the.pdf

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Q4.7. In DNA replication, what is the difference between the leading and lagging strands? In the leading strand, DNA is synthesized 53, while the direction of synthesis in the lagging strand may be either 53 or 35, The leading strand is composed of DNA only, while the lagging strand is composed of both RNA and DNA. After extension, the leading strand is continuous, while the lagging strand is composed of disconnected fragments. The leading strand is synthesized only by DNA polymerase III, while the lagging strand is synthesized only by DNA polymerase I. Q4.8. DNA replication involves a series of steps, including initiation, making primers, extension, and connecting DNA fragmnts. The cell uses different enzymes in a particular order to accomplish these steps. Which of the following represents the correct general order of enzyme use? (Hint: You may want to use the glossary to review terms.) DNA polymerases, helicase, primase, ligase Helicase, ligase, primase, DNA polymerases Helicase, primase, DNA polymerases, ligase Primase, helicase, DNA polymerases, ligase Q4.9. Which of the following segments of DNA would require the most energy to separate the two strands?.

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1.) What does “exonuclease” activity mean? Which enzyme important for DNA polymerization has both 5’ – 3’ AND 3’ – 5’ exonuclease activity? Which is important for proofreading function? 2.) How are the site(s) where replication begin(s) is/are characterized as in both prokaryotes and eukaryotes? What is beneficial about this? Are the sites the same in prokaryotes and eukaryotes? 3.) Arrange the following proteins in the proper order in which they participate in DNA replication. 1 = Primase 2 = Helicase 3 = Single-strand binding proteins 4 = DNA polymerase I 5 =DNA polymerase III 4.) When does DNA replicate in the cell cycle of eukaryotes and prokaryotes? 5.) Why are Okazaki fragments only found on the lagging strand, but not on the leading strand of DNA? 6.) What protein transports the histones of Nucleosomes into the nucleus? 7.) Describe the differences between Topoisomerase I and Topoisomerase II. 8.) During DNA replication, ___________ adds an RNA primer to naked template strands of DNA. Why is this necessary during replication? What fills the gap after removal of this RNA primer? 9.) What is the problem with the ends of linear eukaryotic chromosomes? How does telomerase help the issue? Solution 1. Exonuclease activity means that enzymes work by cleaving nucleotides one at a time from the end (exo) of a polynucleotide chain. A hydrolyzing reaction breaks phosphodiester bonds at either the 3’ or the 5’ ends. DNA polymerase I is the enzyme important for DNA polymerization and has both 5’ – 3’ and 3’ – 5’ exonuclease activity, which is used in editing and proofreading DNA for errors. The 3\' to 5\' can remove only one mononucleotide at a time, whereas the 5\' to 3\' activity can remove up to 10 nucleotides at a time. 2. Sites from which the process of replication starts are different in prokaryotes and Eukaryotes. Prokaryotes have only one origin of replication per DNA molecule, whereas in ekaryotes, there are multiple sites for origin of replication. To initiate DNA replication in Eukaryotes, multiple replicative proteins assemble on and dissociate from these replicative origins. DNA double helix is unwound and an initial priming event by DNA polymerase occurs on the leading strand.To initiate the process of DNA replication, multiple replicative proteins assemble on and dissociate from these replicative origins. The individual factors work together to direct the formation of the pre-replication complex (pre-RC), a key intermediate in the replication initiation process. In case of prokaryotes, Helicase separates the DNA to form a replication fork at the origin of replication where DNA replication begins. Replication forks extend bi-directionally as replication continues. The initiation of DNA replication is mediated by DnaA, a protein that binds to a region of the origin known as the DnaA box. E. coli, has 4 DnaA boxes, each of which contains a highly conserved 9 bp consensus sequence 5\' - TTATCCACA - 3\'. 3. Enzymes that participate in DNa.

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