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Cell Structure and Function
1
Cells
• Smallest living unit
• Most are microscopic
2
Discovery of Cells
• Robert Hooke (mid-1600s)
– Observed sliver of cork
– Saw “row of empty boxes”
– Coined the term cell
3
Cell theory
• (1839)Theodor Schwann & Matthias Schleiden
“ all living things are made of cells”
• (50 yrs. later) Rudolf Virchow
“all cells come from cells”
4
Principles of Cell Theory
• All living things are made of cells
• Smallest living unit of structure and
function of all organisms is the cell
• All cells arise from preexisting cells
(this principle discarded the idea of
spontaneous generation)
5
Cell Size
6
Cells Have Large Surface
Area-to-Volume Ratio
7
Characteristics of All Cells
• A surrounding membrane
• Protoplasm – cell contents in thick fluid
• Organelles – structures for cell function
• Control center with DNA
8
Cell Types
• Prokaryotic
• Eukaryotic
9
Prokaryotic Cells
• First cell type on earth
• Cell type of Bacteria and Archaea
10
Prokaryotic Cells
• No membrane bound nucleus
• Nucleoid = region of DNA concentration
• Organelles not bound by membranes
11
Eukaryotic Cells
• Nucleus bound by membrane
• Include fungi, protists, plant,
and animal cells
• Possess many organelles
Protozoan 12
Representative Animal Cell
13
Representative Plant Cell
14
Organelles
• Cellular machinery
• Two general kinds
– Derived from membranes
– Bacteria-like organelles
15
Bacteria-Like Organelles
• Derived from symbiotic bacteria
• Ancient association
• Endosymbiotic theory
– Evolution of modern cells from
cells & symbiotic bacteria
16
Plasma Membrane
• Contains cell contents
• Double layer of phospholipids & proteins
17
Phospholipids
• Polar
– Hydrophylic head
– Hydrophobic tail
• Interacts with water
18
Movement Across the Plasma Membrane
• A few molecules move freely
– Water, Carbon dioxide, Ammonia, Oxygen
• Carrier proteins transport some molecules
– Proteins embedded in lipid bilayer
– Fluid mosaic model – describes fluid nature of
a lipid bilayer with proteins
19
20
Membrane Proteins
1. Channels or transporters
– Move molecules in one direction
2. Receptors
– Recognize certain chemicals
21
Membrane Proteins
3. Glycoproteins
– Identify cell type
4. Enzymes
– Catalyze production of substances
22
Cell Walls
• Found in plants, fungi, & many protists
• Surrounds plasma membrane
23
Cell Wall Differences
• Plants – mostly cellulose
• Fungi – contain chitin
24
Cytoplasm
• Viscous fluid containing organelles
• components of cytoplasm
– Interconnected filaments & fibers
– Fluid = cytosol
– Organelles (not nucleus)
– storage substances
25
Cytoskeleton
• Filaments & fibers
• Made of 3 fiber types
– Microfilaments
– Microtubules
– Intermediate filaments
• 3 functions:
– mechanical support
– anchor organelles
– help move
substances
26
A = actin, IF = intermediate filament, MT = microtubule
27
Cilia & Flagella
• Provide motility
• Cilia
– Short
– Used to move substances
outside human cells
• Flagella
– Whip-like extensions
– Found on sperm cells
• Basal bodies like
centrioles
28
Cilia & Flagella Structure
• Bundles of microtubules
• With plasma membrane
29
Centrioles
• Pairs of microtubular structures
• Play a role in cell division
30
Membranous Organelles
• Functional components within cytoplasm
• Bound by membranes
31
Nucleus
• Control center of cell
• Double membrane
• Contains
– Chromosomes
– Nucleolus
32
Nuclear Envelope
• Separates nucleus from rest of cell
• Double membrane
• Has pores
33
DNA
• Hereditary material
• Chromosomes
– DNA
– Protiens
– Form for cell division
• Chromatin
34
Nucleolus
• Most cells have 2 or more
• Directs synthesis of RNA
• Forms ribosomes
35
Endoplasmic Reticulum
• Helps move substances within cells
• Network of interconnected membranes
• Two types
– Rough endoplasmic reticulum
– Smooth endoplasmic reticulum
36
Rough Endoplasmic Reticulum
• Ribosomes attached to surface
– Manufacture protiens
– Not all ribosomes attached to rough ER
• May modify proteins from ribosomes
37
Smooth Endoplasmic Reticulum
• No attached ribosomes
• Has enzymes that help build molecules
– Carbohydrates
– Lipids
38
Golgi Apparatus
• Involved in synthesis of plant cell wall
• Packaging & shipping station of cell
39
Golgi Apparatus Function
1. Molecules come in vesicles
2. Vesicles fuse with Golgi membrane
3. Molecules may be modified by Golgi
40
Golgi Apparatus Function
(Continued)
4. Molecules pinched-off in separate vesicle
5. Vesicle leaves Golgi apparatus
6. Vesicles may combine with plasma
membrane to secrete contents
41
42
Lysosomes
• Contain digestive enzymes
• Functions
– Aid in cell renewal
– Break down old cell parts
– Digests invaders
43
Vacuoles
• Membrane bound storage sacs
• More common in plants than animals
• Contents
– Water
– Food
– wastes
44
Bacteria-Like Organelles
• Release & store energy
• Types
– Mitochondria
(release energy)
– Chloroplasts
(store energy)
45
Mitochondria
• Have their own DNA
• Bound by double membrane
46
Mitochondria
• Break down fuel molecules (cellular respiration)
– Glucose
– Fatty acids
• Release energy
– ATP
47
Chloroplasts
• Derived form photosynthetic bacteria
• Solar energy capturing organelle
48
Photosynthesis
• Takes place in the chloroplast
• Makes cellular food – glucose
49
Review of Eukaryotic Cells
50
Review of Eukaryotic Cells
51
52
Molecule Movement & Cells
• Passive Transport
• Active Transport
• Endocytosis
(phagocytosis & pinocytosis)
• Exocytosis
53
Passive Transport
• No energy required
• Move due to gradient
– differences in concentration, pressure, charge
• Move to equalize gradient
– High moves toward low
54
Types of Passive Transport
1. Diffusion
2. Osmosis
3. Facilitated diffusion
55
Diffusion
• Molecules move to equalize concentration
56
Osmosis
• Special form of diffusion
• Fluid flows from lower solute concentration
• Often involves movement of water
– Into cell
– Out of cell
57
Solution Differences & Cells
• solvent + solute = solution
• Hypotonic
– Solutes in cell more than outside
– Outside solvent will flow into cell
• Isotonic
– Solutes equal inside & out of cell
• Hypertonic
– Solutes greater outside cell
– Fluid will flow out of cell
58
59
Facilitated Diffusion
• Differentially permeable membrane
• Channels (are specific) help molecule
or ions enter or leave the cell
• Channels usually are transport proteins
(aquaporins facilitate the movement of
water)
• No energy is used
60
Process of Facilitated Transport
• Protein binds with molecule
• Shape of protein changes
• Molecule moves across membrane
61
Active Transport
• Molecular movement
• Requires energy (against gradient)
• Example is sodium-potassium pump
62
Endocytosis
• Movement of large material
– Particles
– Organisms
– Large molecules
• Movement is into cells
• Types of endocytosis
– bulk-phase (nonspecific)
– receptor-mediated (specific)
63
Process of Endocytosis
• Plasma membrane surrounds material
• Edges of membrane meet
• Membranes fuse to form vesicle
64
Forms of Endocytosis
• Phagocytosis – cell eating
• Pinocytosis – cell drinking
65
Exocytosis
• Reverse of endocytosis
• Cell discharges material
66
Exocytosis
• Vesicle moves to cell surface
• Membrane of vesicle fuses
• Materials expelled
67
68

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Cell Structures and functions