This curriculum vitae outlines the career and accomplishments of Tarun K. Dhar, a scientist and head of the Drug Development, Diagnostics and Biotechnology Division at the Indian Institute of Chemical Biology in Kolkata, India. It details his education, awards, professional appointments rising to the position of Scientist G, research interests in developing new immunochemical methods and immunoassays, notable research contributions including developing new signal amplification technologies for ELISA, publications, patents, and professional affiliations.

1. CURRICULUM VITAE
Tarun. K. Dhar, PhD
Scientist G and Head
Drug Development, Diagnostics & Biotechnology Division
Indian Institute of Chemical Biology
4, Raja S. C. Mullick Road,
Jadavpur, Calcutta-700032, INDIA
Phone: 033-2473-5112; 033-24995719 (Off.)
Fax: 033-24730284; 033-24735197
E.mail: tkdhar@iicb.res.in
Web site: www.iicb.res.in
EDUCATION:
M.Sc 1975 University of Lucknow, Lucknow, India
Ph.D 1980 University of Calcutta, Kolkata, India.
AWARDS/ HONOURS:
Recipient of long term CSIR-DAAD fellowship (West Germany) during 1984-1986.
PROFESSIONAL APPOINTMENTS:
Month/ 1979- month/1984 Scientist – B
Indian Institute of Chemical Biology,
Jadavpur, Kolkata 700032, India
Month/ 1984- month/1989 Scientist – C
Indian Institute of Chemical Biology,
Jadavpur, Kolkata 700032, India
Month/ 1984- month/1986 DAAD Fellow
Free University of Berlin,
Berlin, Germany
Month/ 1989- month/1995 Scientist – EI
Indian Institute of Chemical Biology,
Jadavpur, Kolkata 700032, India
Month/ 1995- month/2001 Scientist – EII
Indian Institute of Chemical Biology,

2. Jadavpur, Kolkata 700032, India
Month/ 2001- month/2005 Scientist – F
Indian Institute of Chemical Biology,
Jadavpur, Kolkata 700032, India
Month/ 2005- present Scientist – G
Indian Institute of Chemical Biology,
Jadavpur, Kolkata 700032, India
RESEARCH INTEREST:
• Synthesis of haptens, immunogens necessary for polyclonal antibody
production;
• Synthesis of enzyme conjugates, protein conjugates;
• Immunological method development (including ELISA, membrane
immunoassay, membrane-based device);
• Development of a variety of immunoassay formats;
• Development of new immunochemical methods for the detection of
mycotoxins in foods; and
• Integration of immunochemical methods with traditional analytical
procedures.
Notable Research Contributions:
1. Development of innovative sandwich immunoassay of small molecules, novel
carrier protein for raising highly specific and high titre antibody against small
molecules and an improved signal amplification technology termed Super-CARD
and its application in rapid ELISA and ultrasensitive Dot-ELISA detection for
antigen/antibody.
2. The structure elucidation of the phytotoxin, phaseolinone and the development of
its immunoassay is a major contribution to phytopathology.
3. A simple highly cost-effective analytical device for performing on-site
immunoassay on a batch of 12 samples. The device consists of a membrane strip
with multiple antibody-immobilized zones, one end of which is attached to a
polyethylene card. Its applicability for detection of aflatoxin B1 within 12 min in
different foodstuffs has been demonstrated.
4. A novel strategy for carrying out both sample cleanup of interfering substances
and quantitative determination of the analyte in the same immunoassay system.

3. 5. A simple and highly cost-effective method for performing simultaneous rapid
detection of AFB1 and OA in chili samples in a single assay.
PROFESSIONAL SOCIETIES:
Member of Indian Society of Biological Science
Member of Indian Immunology Society
Member, Science Congress Association
Member, Indian Association for the Cultivation of Science, Kolkata
PEER REVIEW/EDITORAIL ACTIVITY:
Regular reviewer of Analytical Chemistry
Journal of Agricultural and food contaminants
Analytica Chimica Acta
J. Chromatogr.
Mycotoxin Research
Research grant reviewer for
Department of Science and Technolgy (DST), Government of India.
Council of Scientific and Industrial Research (CSIR), Government of
India.
Department of Biotechnology (DBT), Government of India, Delhi
Global Innovation and Technology Alliance, Delhi.
PUBLICATIONS:
1. Ali, E, Chakravarty, A. K., Dhar, T. K. and Pakrashi, S. C. (1978)
Solaseaforthine and iso-solaseaforthine, two novel 3-amino pregnanes from
Solanum seaforthianum. Tetrahedran Letters, 3871 - 3874.
2. Chakravarty, A. K. Dhar, T. K. and Pakrashi, S. C. (1978) Hispigenin, A novel
22β O-spirostane from Solanum hispidum. Tetrahedran Letters 3875 - 3878.

4. 3. Pakrashi, S. C. Chakravarty, A. K., Dhar, T. K., Ali, E. and S. Dan (1978) Novel
3-amino solanidanes from Solanum gigantium. J. Indian Chem. Soc.(spl. issue)
55, 1109 - 1113.
4. Chakravarty, A. K., Dhar, T. K. and Pakrashi, S. C. (1980) Solaspigenin and
Neosolaspigenin, two new spirostane sapogenins from Solanum hispidum.
Phytochemistry 19, 1249 - 1251.
5. Chakravarty, A. K.,Saha, C. R., Dhar, T. K. and Pakrashi, S. C. (1980)
Constituents of some Solanum species. Mass spectra of Nuatigenin and
Isonuatigenin. Indian J. Chem. 19B, 468 - 472.
6. Dhar, T. K., Siddiqui, K. A. I. and Ali, E.. (1982) Structure of phaseolinone, A
novel phytotoxin from Macrophomina phaseolina. Tetrahedron Letters, 5494 –
5497.
7. Ghosh, M., Dhar, T. K., Ali, E. and Bachhawat, B. K. (1983) Homogeneous
enzyme immunoassay of estriol at picogram level. Clinica Chim. Acta. 128, 223
– 231.
8. Dhar, T. K., Muller, C. and Schoneshofer, M. (1985) Determination of
dehydroepiandrosterone sulfate in plasma by a one-step enzyme immunoassay
with a microtitre plate. Clin. Chem. 31, 1876 – 1879.
9. Dhar, T. K., Voβ, E. and Schoneshofer, M. (1986) Enzyme immunoassay of
serum cortisol using a new transferable needle lid technique. Clinica Chim. Acta
157, 231 – 238.
10. Dhar, T. K., Voβ, E and Schoneshofer, M. (1986) Simultaneous enzyme
immunoassay of cortisol and dehydroepiandrosterone sulfate using a transferable
solid phase system. J. Steroid Biochem.25, 423 – 427.
11. Schoneshofer, M., Kage, A., Eisenschmid, B., Heilmann, P. Dhar, T. K. and
Weber, B. (1986) Automated, liquid chromatographic determination of the 20-
dihydro-isomers of cortisol and cortisone in human urine. J. Chromatography
380, 267 – 274.
12. Schoneshofer, M., Dhar, T. K. and Ioanides, D. (1986) Determination of total
urinary estriol by on-line liquid chromatography with ultraviolet detection. Clin.
Chem. 32, 1948 – 1951.

5. 13. Holfeld, C., Dhar, T. K., Muller, C. and Schoneshofer, M. (1986) Enzyme
immunoassay of serum aldosterone using a transferable needle lid technique. J.
Clin. Chem. and Clin. Biochem. 24, 762.
14. Dhar, T. K. and Schoneshofer, M. (1987) A fully automated method for sample
clean up prior to chromatographic and immunological analysis. J. Clin. Chem.
Clin. Biochem. 25, 241 – 244.
15. Bhattacharya, G. Dhar, T. K., Bhattacharya, F. K. and Siddiqui, K. A. I. (1987)
Mutagenic action of phaseolinone, a mycotoxin isolated from Macrophomina
phaseolina. Aust. J. Biol. Sci. 40, 349 – 353.
16. Dhar, T. K. , Samanta, A. K. and Ali, E. (1988) Homogeneous enzyme
immunoassay of estradiol using 3- O- carboxymethyl ether as hapten. Steroids 51,
519 – 526.
17. Dhar, T. K. and Ali, E. (1992) Direct microtitre plate enzyme immunoassay of
testosterone in unextracted serum. J. Immunol. Methods 147, 167 – 172.
18. Ali, E., Sengupta, J. and Dhar, T. K. (1992) Sandwich immunoassay of small
molecules I : Investigation with testosterone as a model hapten. J. Immunol.
Methods 147, 173 – 179.
19. Sengupta, J., Dhar, T. K. and Ali, E. (1992) Sandwich immunoassay of small
molecules II : Effects of heterology and development of a highly specific and
sensitive enzyme immunoassay of tesosterone. J. Immunol. Methods 147, 181 –
188.
20. Bhattacharya, D., Dhar, T. K. and Ali, E. (1992) An enzyme immunoassay of
phaseolinone and its application in estimation of the amount of toxin in
Macrophomina phaseolina infected seeds. Appl. Envir. Microbiol. 58, 1970 –
1974.
21. Bhattacharya, D. Dhar, T. K. and Ali, E. (1994) Inhibition of seed germination of
Macrophomina phaseolina is related to phaseolinone production. J. Appl. Bact.
77, 129 – 133.
22. Das Sarma, J., Duttagupta, C. Ali, E. and Dhar, T. K. (1995) Antibody to folic
acid : Increased specificity and sensitivity in ELISA by using epsilon-
aminocaproic acid modified BSA as the carrier protein. J. Immunol. Methods

6. 184, 1 – 6.
23. Das Sarma, J., Duttagupta, C. Ali, E. and Dhar, T. K. (1995) Direct microtitre
plate enzyme immunoassay of folic acid without heat denaturation of serum. J.
Immunol. Methods 184, 7 - 14.
24. Das Sarma, J., Duttagupta, C. Ali, E. and Dhar, T. K. (1995) Improved
microbiological assay for folic acid based on microtitre plating with
Streptococcus faecalis. 78, 1173 – 1177.
25. Das Sarma, J., Duttagupta, C. Ali, E. and Dhar, T. K. (1996) Estimation of folic
acid in mutivitamin formulation by enzyme immunoassay. Indian J. Pharm. Sci.
58, 32 – 36.
26. Bhattacharya, R., Bhattacharya, D. and Dhar, T. K. (1999) A novel signal
amplification technology for ELISA based on catalyzed reporter deposition.
Demonstration of its applicability for measuring aflatoxin B1. J. Immunol.
Methods 230, 71 – 86.
27. Bhattacharya, D., Bhattacharya, R. and Dhar, T. K. (1999) A novel signal
amplification technology based on catalyzed reporter deposition and its
application in a Dot-ELISA with ultra high sensitivity. J. Immunol. Methods 227,
31 – 39.
28. Pal A and Dhar T. K. (2004) An analytical device for on-site immunoassay.
Demonstration of its applicability in semiquantitative detection of aflatoxin B1 in
a batch of samples with ultrahigh sensitivity. Anal Chem. 76 (1): 98-104.
29. Pal A., Acharya D., Saha D. and Dhar T. K. (2004) Development of a
membrane-based immunofiltration assay for the detection of T-2 toxin. Anal
Chem. 76 (14): 4237-40.
30. Pal A., Acharya D., Saha D., Roy D. and Dhar T. K. (2005) In situ sample
cleanup during immunoassay: a simple method for rapid detection of aflatoxin B,
in food samples. J Food Prot. 68 (10): 2169-77.
31. Saha D., Acharya D and Dhar T. K. (2006) Method for homogeneous spotting of
antibodies on membranes: application to the sensitive detection of ochratoxin A.
Anal. Bioanl. Chem. 385, 847-854.
32. Saha D., Acharya D., Roy D. and Dhar T. K. (2007) Filtration-based tyramide

7. amplification technique: A new simple approach for rapid detection of aflatoxin
B1, Anal. Bioanal. Chem. 387: 1121-1130
33. .
34. Saha D., Acharya D., Roy D., Shrestha D. and Dhar T. K. (2007) Simultaneous
enzyme immunoassay for the screening of aflatoxin B1 and ochratoxin A in chili
samples, Anal. Chim. Acta 584: 343-349.
35. Acharya D., Saha D., Roy D., Jain P. and Dhar T. K. (2008) Filtration –based
staining of proteins on membranes. Anal. Biochem. 379: 121-123.
36. Acharya D. and Dhar T. K. (2008) A novel broad-specific noncompetitive
immunoassay and its application in the determination of total aflatoxins. Anal.
Chim. Acta 630: 82-92.
PATENTS :
Indian
1. A process for the preparation of a novel N-17β ol androsten-4-(3-Thiopropionyl)
N-2- (Testosterone-3-Iminoxy)acetyl derivative of aliphatic diamine (No.
175619).
2. An improved process for the preparation of microtitre plate useful for enzyme
immunoassay of Testosterone in serum (No. 173970).
3. A process for the preparation of a novel N, N’-bis-17β ol androsten-4-(3-
Thiopropionyl) derivative of aliphatic diamines (No. 175369).
4. A process for the preparation of a novel N, N’-bis-2 (testosterone-3-
Imminoxy)acetyl derivative of aliphatic diamines (175618).
5. An improved process for the preparation of microtitre plate useful for sandwich
immunoassay for haptens (No. 173999).
6. A process for the preparation of electron rich proteins useful for ultrasensitive
detection of biologically important molecules (No. 186427).
7. An improved process for the preparation of solid phases useful for ultrasensitive
visual detection of diagnostically important antigens or antibody (No. 185767).
8. A process for the preparation of electron rich proteins useful for ultrasensitive
detection of biologically important molecules (No. 185769).

8. 9. A highly cost-effective analytical device for performing immunoassays with ultra
high sensitivity (No. 219446).
International
1. A highly cost-effective analytical device for performing immunoassays with ultra
high sensitivity (USA No. 7087389).
2. A highly cost-effective analytical device for performing immunoassays with ultra
high sensitivity (USA div. No. 7312028)
3. A highly cost-effective analytical device for performing immunoassays with ultra
high sensitivity (Denmark No. 10106409.8)