Introduction: Welcome to "Biochemistry Techniques 101: A Beginner's Lecture" presented by Tahaa Saeed. In this video, we'll dive into the fundamental techniques used in biochemistry, perfect for those new to the field. Whether you're a student or just curious about the subject, this lecture will provide you with a solid foundation. Don't forget to like, subscribe, and hit the notification bell to stay updated on our latest biochemistry tutorials! Share this video with anyone who's interested in mastering the basics of biochemistry techniques.

1. BIOCHEMISTRY
TECHNIQUES
02) How to solve a Scientific Problem?
Tahaa Saeed
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2. Steps for solving a Scientific Problem
• Clearly define the
problem you want
to solve. Make sure
it's specific and
well-formulated.
Identify the
Problem
• Gather relevant
information about
the problem.
Background
Research •Based on your
background research,
develop one or more
hypotheses that
propose possible
solutions.
Formulate
Hypotheses
• Test your hypothesis
by designing an
experiment
Experimentation
•Use appropriate
statistical and
analytical tools to
process and interpret
the collected data.
Analyze Data and
draw Conclusions
•Report your findings
and results in a journal
Reporting the
results
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3. Examples of Scientific Problem
A protein is showing catalytic
activity and it has three domains.
We have to check which domain
has catalytic activity.
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4. Identify the Problem
Which protein domain has catalytic
activity?
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5. Background Research
Collect relevant information about
the protein. Read scientific
literature, articles, and books to
understand structure and function
of protein.
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6. Formulate Hypotheses
The protein's catalytic activity is localized
within one of the three domains, and the
other two domains are non-catalytic and
serve as structural or regulatory
elements.
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7. Experimentation
Protein Expression
and Purification
Domain
Identification and
Isolation
Expression and
Purification of
Domain Constructs
Catalytic Assays

8. Analyze Data and draw Conclusions
• Analyze the results of the experiments catalytic activity of each
domain and the full-length protein.
• Based on the results, conclude which domain, if any, exhibits catalytic
activity.
Reporting the Results
• If your findings are significant, consider publishing your results in a
scientific journal
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9. Examples of Scientific Problem
Genetics and Genomics
Identify the genetic mutations responsible for
inherited neurodegenerative diseases like
Alzheimer's, Huntington's, and Parkinson's,
and elucidate their molecular mechanisms.
Ecology and Environmental Biology
Investigate the impact of climate change on
the migratory patterns and breeding success
of Arctic seabirds to inform conservation
efforts in a changing ecosystem.
Cell Biology
Elucidate the signaling pathways involved in
cell cycle regulation and understand how
dysregulation contributes to cancer
development.“
Microbiology
Study the antimicrobial resistance
mechanisms in pathogenic bacteria to
develop new strategies for combating drug-
resistant infections.
Botany
Examine the genetic basis of drought
tolerance in crop plants and develop drought-
resistant varieties to address water scarcity in
agriculture.
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