Developing copper nanoparticle and embedding them into bacterial nanocellulose for wound dressing against gram positive and gram negative bacteria

1. DEVELOPING COPPER NANOPARTICLE AND EMBEDDING THEM INTO
BACTERIAL NANOCELLULOSE FOR WOUND DRESSING AGAINST
GRAM POSITIVE AND GRAM NEGATIVE BACTERIA.
SOWMYA JAISWAL
NUCSER,NITTE(MANGALORE)

2. • CHRONIC WOUNDS LIKE DIABETIC CHRONIC ULCER AND BURN
WOUNDS ARE SUSCEPTIBLE TO ATTACK BY BACTERIA.
• PROPER DRESSING ENSURES THAT THERE IS NO INFECTION.
CHRONIC DIABETIC FOOT ULCER BURN WOUND

3. • Bacterial cellulose is already been used for wound dressing
because :
 It allows easy absorption of exudates from wound site
 It accelerates wound healing
 It protects wound from infection
• The idea is to combine wound healing properties of
nanocellulose with antibacterial properties.
AIM OF RESEARCH

4. OBJECTIVE
• Little research has been done on combining nanoparticles
and nanocellulose.
• Doing this can increase the wound dressing’s effectiveness to
fight infections.
• The resulting copper embedded nanocellulose has several
advantages:
 The antibacterial rate is about 99.99 %.
 It is cheap and effective method.
 It can easily be produced at an industrial scale.
 The method works for antibiotic resistant bacteria,
therefore its future proof.

5. CONCEPT
• Experiments have suggested that nanoparticles have a special
mechanism by which they target bacteria.
• Copper nanoparticles first target the cell wall of bacteria and
caused damage.
• Once they enter the cell, they cause DNA damage by
releasing reactive oxygen molecules.

6. • The expected duration of research is 9 months.
• The budget required is 5,20,090.00 Rupees.
• Special equipments required are a Incubator, Ph meter, Polycarbonated
membrane and Magnetic stirrer.
Particulars Cost
Salaries 2,70,000.00
Travel and others 5,000.00
Special equipment requirements 51,000.00
Resource requirement 12,000.00
Dissemination cost 1,12,000.00
Consumable 29,820.00
Miscellaneous 10,000.00
Total 5,20,090.00
BUDGET

7. METHODOLOGY
Steps to prepare the nanocellulose film:
 The culture medium needs to be mixed with bacteria, and poured
into the vessels.
 Put the vessel in the fermenter under temperature 30 degree
Celsius and 4.5 to 7.5.
 Incubate for 2 to 20 days.
 At the air – culture medium interface hydrogel formation takes
place because of accumulation of gelatinous cellulose membrane.
 To increase the thickness of the film, the culture time needs to be
increased.
 This is collected and washed in a mixture of sodium hydroxide and
acetic acid, until translucent suspension in water is obtained

8. METHODOLOGY
Steps for embedding copper nanoparticles into nanocellulose:
 Glycerol is added to improve the flexibility of NC film.
 Cu (NO3)2.3H2O (0.015 M) is added at speed of one drop per 2
seconds.
 NaBH4 solution 0.1 M is added and stirred for 2 hours under 75
degree Celsius.
 The colour changes from yellow to orange and finally brick red.
 To remove excess water polycarbonate membrane filter is used.
 Then the film is placed between two glass sheets and dried at 85
degree Celsius for 20 minutes.
 To prevent oxidation and corrosion the film is dipped in 1%
benzotriazole solution at Ph overnight.

9. EXPECTED OUTCOME
The film obtained can be covered with foam and bandage can
be applied to hold them together.

10. Thank you