This is bout Begomovirus which is responsible for many mosaic viruses diseases throughout the world like tobacco mosaic, tomato mosaic etc. This is an Research result which is done in NCR region in India. where the viruses presence is looked for around the agricultural land weed plants.

1. Guide: Dr. Vanita Chandel
Amity Institute of Virology
&
Immunology
By: Km. Soma Majumdar
PGDCVI
A12173422004

2. Identify the virus infecting
weed plants in NCR region
Major NTCC project

3. Introduction
• Weeds are potential sources of primary inoculums of viruses and
play an important role in their persistence and spread.
• Visually recognizing viral infections in plants can be challenging,
often leading to mistaking as bacterial infections or nutrient
deficiencies.
• Aim for this study was identifying the virus infecting weed plants.
• The plant samples were collected from Noida and Ghaziabad
districts of Uttar pradesh.
• I try to be consistent when choosing the sample: discolouring and
curling of young leaves.

4. • The techiques i have used for this is simple Plant DNA
extaction by CTAB method and Agarose gel
elcetrophoresis.
• Polymerase chain reaction and Agarose gel
electrophoresis for the presence of viruses in the sample.
• The Plant sample mainly were Croton, Solanum nigrum,
Amaranthus, Parthenium hysterophorus, Mimosa invisa.

6. F. Mimosa invisa
D. Solanum nigrum

8. Polymerase chain reaction
Reagent Amount
Water 64 micro liters
Buffer (10 x) 10 micro liters
dNTP’s 4 micro liters
Primer: Reverse &
forward
8 micro liters (4 micro liters
each)
Taq polymerase 2 micro liters
Template 1 micro liters

9. Condition (Tm) 48 oC
Initial denaturation 3 min at 94 oC
Cycle denaturation 30 sec at 94 oC
Annealing 30 sec at 48 oC
Extension 1 minute at 72 oC
Final Elongation /Extension 10 minutes at 72 oC
Store 4 oC
Repeated
30
times

10. Result
1, 2, 3, 4, 6, 7, 9, 10 were
positive for
Begomovirus Cp at 800
bp.
S. No Sample name
1 Croton
2 Solanum nigrum
3 Solanum nigrum
4 Solanum nigrum
6 Mimosa invisa
7 Croton
9 Amaranthus viridis
10 Parthenium hysterophorus

11. Major Begomovirus in Asia
• Tomato leaf curl New Delhi virus (ToLCNDV)
• Cotton leaf curl disease (CLCuD)
• African cassava mosaic virus (ACMV)
• Potato yellow mosaic virus
• Mung bean yellow mosaic virus
• Chili leaf curl India virus

12. Weeds
They are cosmopolitan
in nature.
They reduce crop yield
and degrade quality of
the produce.
They also cause health
hazards and loss of
biodiversity.
Weeds account for about
one-third of the total losses

13. Thank you