Shamili Sammohi has extensive experience in in-vivo, in-vitro, and analytical techniques including mice surgeries, cell culture, Western blotting, and HPLC. She has worked as a research assistant at the University of Cincinnati studying nociception in mice and performed techniques like brain surgeries, DRG culture, immunohistochemistry, and electrophysiology. She holds an MS in Pharmacology and Toxicology from Wright State University and a BS in Pharmacy from Sahasra Institute of Pharmaceutical Sciences in India.

1. Shamili Sammohi
306 Stonegate Dr, Columbus, IN, 47201
Ph: 859-473-5275 E-mail: shamilisammohi@gmail.com
LinkedIn: www.linkedin.com/in/shamilisammohi/
Summary:
 Experienced in In-vivo, In-vitro and Analytical techniques.
 In-vivo Techniques:
Mice brain surgeries using stereotaxic apparatus,perfusion, genotyping, maintaining mice colonies,
Electrophysiology, optogenics, IP, SC and Intrathecal (IT) injections in conscious mice, CFA
injections to the paw, SNi and SNL surgeries. Behavior analysis including Heat Plantar test and
Von Frey filaments for nociception, hot and cold plate testing, mechanical stimulus testing, startle
response test, X and Y maze test and tail suspension test.
 In-vitroTechniques:
Cell culture, Western Blotting, SDS-PAGE, Immunocytochemistry (ICC), qRT-PCR, IFA, PAS
and DAPI staining, Sandwich ELISA, Mass-Spectrometry like MALDI TOF/TOF, LCMS,
MS/MS, RNA extraction and quantification, protein purification, TLC, Gel-Electrophoresis,
molecular microbiological techniques like aseptic technique, PCR, bacterial and adenocarcinoma
lung cell cultures, reagent preparation and particulate testing
 Analytical Techniques:
Drug quality analysis using HPLC, GC, UV-Vis.
 Excellent communication, problem solving and organizational skills with a high level of attention
to detail, proven ability to work as part of a cross-functional team, troubleshooting
Education:
 MasterofScience inPharmacology&Toxicology, Wright State University, Dayton OH (July 15)
 Bachelor ofScience in Pharmacy, Sahasra Institute of Pharmaceutical Sciences, India (July 12)
Work Experience:
Research Assistant at University OfCincinnati, School OfMedicine, Department ofAnesthesiology,
Cincinnati, Ohio (Dec 2015 – present)
 Performed animal behavior analysis with transgenic and WT mice using Heat Plantar Test, Von
Frey Filaments for Nociception, hot and cold plates and mechanical stimulus using Any-Maze
software.
 Performed mice brain surgeries including implanting cannula and injecting AAV2,AAV8,Dil and
Fluoro Ruby into the Triangular Posterior nucleus of the Thalamus (PoT), Insula and Anterior
Cingulate Cortex (ACC). Perfused mice to collect brain, spinal cord and DRG and sectioned them
using cryostat.
 Performed Spared Nerve Injury (SNi) and Spinal Nerve Ligation (SNL) surgeries on mice.
 Performed Human DRG culture (obtained from Life Centre - Donor organ recovery for Greater
Cincinnati area), conducted further experiments and acquired knowledge about consent forms.
 Designed and built new apparatus for hot and cold testing and mechanical stimulus experiments.
 Managed mice colonies, lab inventory and documented all the work performed in the lab following
GLP, GCP, OSHA, IRB guidelines.
 Trained new post-doctoral, graduate and undergrad research assistants in various lab techniques.
Mentored undergrad students on research projects.
 Performed statistical analysis of experimental data using Prism and Excel.
 Techniques Used: Dorsal Root Ganglion (DRG) Prep and culture, Immunohistochemistry,
Electron Microscopy, Calcium imaging, Patchclamp electrophysiology, optogenetics, microscopic
imaging, image processing and image analysis, Intrathecal(IT), IP and Sub-cutaneous injections in
conscious mice, CFA injections to the paw, Anesthesia, Genotyping, DNA and RNA isolation,
PCR, Western blotting, Gel Electrophoresis.
Research Experience:
Graduate Assistant, Proteomic Laboratory, Boonshoft School ofMedicine, Wright State University,
Dayton, OH

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As a graduate student in Proteomic laboratory: Organized, managed and ordered laboratory materials for
low cost; troubleshot and handled research problems efficiently; maintained records of experiments and
results; safeguarded confidential data and samples; followed lab safety, cell-based assay development in
microtiter plates
Effect of Terbutaline, Progesterone and Leptin on Type II Alveolar cells (Jan 2014-July2015)
 Studied the effect of Terbutaline, Progesterone and Leptin on increase in surfactant production in
alveolar type II cells (Pneumonocytes) as an in-vitro study for estimating the beneficial drug levels
in premature babies and others prone to respiratory distress syndrome (RDS).
 Corticosteroids, betamethasone and dexamethasone are used as positive controls and also in
combination with each of the drugs to estimate the individual and combinatorial effects on
surfactant production using human adenocarcinoma cell line (NCI-H441) from ATCC.
Effect ofTocolytic medications on Synthesis and Secretion ofSurfactant in an In-Vitro Human Lung
cell model (May-November 2014)
 Studied effect of Tocolytic drugs Indomethacin, Nefidipine and Magnesium Sulfate on Alveolar
type II cells using Betamethasone (BMZ) as control.
 Collaborative clinical project with OB/GYN MFM Fellow ZiadHaidar at Miami Valley Hospital.
Graduate Assistant, BehavioralandNeuro Science Laboratory, WSU,Dayton,OH (Sept 2013-Dec2013)
Behavioral Analysis of NKCC Knock out and Wild Type Mice
 Conducted behavioral studies of NKCC Knock out Type II diabetic mice (Hetero, Homo) and wild
type mice using startle response test, X and Y maze test and tail suspension test.
 Analyzed mice data using Statistica and SPSS software, acquired knowledge of consent forms.
Graduate Assistant, Wright State International Gateway, Wright State University, Dayton, OH
 Worked for International admissions office to accelerate the rate of admissions, conducted surveys
on international student issues and delivered reports to administration, assessed problems based on
surveys, analyzed and innovatively implemented problem solving student retention strategies.
Summer Intern, Acacia Life Sciences Pvt. Ltd. Hyderabad, India (May 2011-Aug 2011)
 Analyzed the quality of Troxerutin drug extracted and processed from a root drug called Rutin
using chromatographic techniques such as HPLC and GC.
 Worked closely with QC, QA and R&D departments of natural products division.
Teaching Assistant, Sahasra Institute ofPharmaceutical Sciences,India (Dec 2010-May 2011)
 Conducted experiments on smooth muscle and cardiac muscle tissue of frog.
 Analyzed Dose - Response curves of tissues for various adrenergic and cholinergic blockers.
Paper Publications:
 “Effect of Tocolytics on Surfactant Secretion when Administered with Betamethasone:an in Vitro
Study” in American Journal of Perinatology (May 2015).
Published Abstracts:
 Presented posters at Ohio Valley Chapter of the Society of Toxicology, Central Research
Forum, Fourth Annual Student Congress of Indian Pharmaceutical association (IPA)
Trainings:
 Biohazard and chemical hazard training from WSU Environmental Health Services Dept. Animal
handling, Virus trainings from Univ. of Cincinnati Laboratory Animal Medical Services (LAMS).
Academic Achievements:
 Awarded Merit Scholarship for excellence in academics at Wright State University, Jan-Aug 2014.
 Awarded Certificate of Merit at Sahasra Institute of Pharmaceutical Sciences, Aug 2010.
Leadership Activities:
 Elected Treasurer and Grants Chair of Graduate Student Assembly, Wright State University.
 Founding member of the social service organization ‘Amma Mighty Hopes’ established to help
orphans and oppressed people in Warangal, India in collaboration with Yuva Jagruthi.