Prathamesh R. Mahajan has over 10 years of experience in toxicology and pharmacology. He received two Master's degrees from St. John's University in toxicology and pharmacology. As a toxicologist at L'Oreal for the past three years, his responsibilities include reviewing materials for safety, conducting risk assessments, and providing product approvals. Previously he conducted research on the toxicity of pesticides and the anti-inflammatory effects of drugs. He has published papers and presented his research at conferences.

1. Prathamesh R. Mahajan
836, Cricket lane, Woodbridge NJ 07095
E-mail: mahajanprathamesh@gmail.com Phone: (201) 406-2589
EDUCATION:
Master of Science, Pharmacology (August 2011- December 2014)
St. John’s University, Queens, New York
Master of Science, Toxicology (August 2008- July 2011)
St. John’s University, Queens, New York
Bachelor of Pharmacy (August 2004-May 2008)
Mumbai University, Mumbai- India
WORK EXPERIENCE:
Toxicologist at L’Oreal (March 2015-present):
Responsibilities:
• Review raw material using OECD toolbox and Tox Tree to allow usage, and or threshold increase within the
makeup métier
• Evaluate toxicological profile of current and new raw materials based on margin of safety, quantitative risk
assessment and dermal sensitization threshold
• Actively involved in discussion with various support groups such as DIMP (raw material experts), chemists and
regulatory department of the acquisitions to fetch data on the raw material’s toxicity profile
• Work with various development groups and counterparts from other countries to conduct risk assessment on a
formula, and advice on clinical testing strategy, to help launch a safe product, within the launch timeline
• Help provide product safety approvals on internal, and new L’Oreal acquisitions by reviewing clinical and in-
vitro safety data.
• Provide appropriate clinical safety support and/or claim substantiation for finished products
• Provide project updates on the internal and new L’Oreal acquisitions to the higher management involving
various support groups
• Help develop clinical testing methods and protocols based on GCP (Good Clinical Practices) standards
• Audit and report clinical studies conducted at CRO (Clinical Research Org) to ensure they are meeting the
L’Oreal, FDA and EU (European zones) guidelines
TECHNICAL SKILLS: I have 6 years of laboratory experience in the cGMP environment.
1) Worked independently (under the guidance of my principal investigator) on the project “Evaluation of toxicity of
Maneb (pesticide) on the hippocampal astrocytes” from 2008-2011 as a research student during my Masters in
Toxicology. My project consisted of
• Establishing and maintaining primary cultures
• Establishing LC50 of Maneb in the NRF-2, and NRF-2 knockout astrocytes by performing cytotoxicity assays
such as MTT assay, trypan blue assay, Luminescence Assays.
• Establishing various endpoints of Apoptosis/ Necrosis using fluorescence Assays. Using light microscopy and
Scanning electron microscopy to document the morphological changes in the cellular structure due to the effect
of Maneb (Cellular biology)
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2. This work helped to conclude that high use of Maneb by the farmers and accidental exposure to high amount of Maneb
could be one of causes of Alzhimer’s disease.
2) Worked independently (under the guidance of my principal investigator) on the project “The Role of Endothelin-1 in
Endotoxin-Triggered Release of Placental Pro-inflammatory Cytokines.” from 2011-2014 as a research student during
my Masters in Pharmacology. The main purpose of this project was to show the anti-inflammatory effect of BQ-123
(endothelin-1 receptor antagonist) to help avoid the pre-term births. This project consisted of
• Establishing human placental explants from full term human placenta, tissue sampling, maintaining the ex-vivo
system and protein extraction by tissue lysis (Ex-Vivo), maintaining and working with cell-line (in-vitro) and
whole Animal Pharmacology, including drug administration (i.p), blood collection by cardiac puncture (In-Vivo)
• Performing protein estimation assays including BCA assay and Bradford Assay, Western Blots, ELISA,
ELISpot, Microarrays, Immunohistocytochemistry, H/E staining (Biochemical assays)
• Identifying the genes involved in the inflammation performing RNA extraction, and real-time qPCR. The RNA
was also quantified using highly sophisticated NANOdrop technology.
This work helped me to conclude that evaluation of inflammatory mediators affected by ET-1 may lead to the
identification of novel targets for preventive therapy for Preterm births.
Some of the other technical skills I acquired during my job as a teaching assistant are DNA isolation and transfection
study, enzyme kinetic assay and Thin layer chromatography (TLC), High performance liquid chromatography (HPLC)
ACADEMIC APPOINTMENTS:
Teaching Assistantship (College Of Pharmacy And Allied Health Professions, St. John’s University, Queens, New
York)
• Successfully conducted Anatomy & Physiology labs, Physical Chemistry and for Doctor of Pharmacy Students.
(August 2011 - January 2014)
• Mentored and guided 4th
year Doctor of Pharmacy student during semester long research internships
(July 2013 - December 2014)
ABSTRACTS AND POSTER PRESENTATIONS:
• Mahajan P, Donepudi R, Einstein F, Reznik S. The Role of Endothelin-1 in Endotoxin-Triggered Release of
Placental Pro-inflammatory Cytokines. 60th
Annual Society of Gynecological Investigations meeting(March 2013)
• Mahajan P, Donepudi R, Einstein F, Reznik S. The Role of Endothelin-1 in Endotoxin-Triggered Release of
Placental Pro-inflammatory Cytokines. 53rd
Annual Society of toxicology meeting (March 2014)
• Mahajan P, Donepudi R, Lopes N, Einstein F, Reznik S. The Role of Endothelin-1 in Endotoxin-Triggered Release
of Placental Pro-inflammatory Cytokines. 33rd
Annual GRASP conference (June 2014)
• Mahajan P, Donepudi R, Lopes N, Einstein F, Reznik S. The Role of Endothelin-1 in Endotoxin-Triggered Release
of Placental Pro-inflammatory Cytokines. 34th Annual American society of reproductive immunology meeting (June
2014)
AWARDS AND HONORS:
• Travel award at 60th
Annual Society of Gynecological Investigations meeting, Florida (19th
-23rd
March 2013)
• 2nd
runners up poster award at 34th
Annual American society of reproductive immunology meeting (June 2014)
PUBLICATIONS:
• Vibhuti Vyas, Charles R. Ashby Jr., Nicole S. Olgun, Sruthi Sundaram, Oluwabukola Salami, Swapna Munnangi,
Ryan Pekson, Prathamesh Mahajan and Sandra E. Reznik (2014). Inhibition of Sphingosine Kinase Prevents
Preterm Birth.
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3. • Prathamesh Mahajan, Ryan Pekson, Sandra E. Reznik. The Role of Endothelin-1 in Endotoxin-Triggered Release
of Placental Pro-inflammatory Cytokines (In progress).
References available on request
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