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© European Union, 2002-2013 | Page 1 / 3 
Amarish Kumar Sharma 
Norrebrogade 51, st 0207...
 Investigated the effect of Hepatocyte Nuclear Factor-4α phosphorylation and its transactivation ...
© European Union, 2002-2013 | Page 3 / 3 
 Expertise in Animal Cell Culture; Cell line ...
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Europass CV 3PAmarish Sharma 2014-Denmark


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Europass CV 3PAmarish Sharma 2014-Denmark

  1. 1. © European Union, 2002-2013 | Page 1 / 3 Amarish Kumar Sharma Norrebrogade 51, st 0207, Copenhagen 2200, Denmark. 0551-650 5550 (India) +45 71 36 92 93 (Copenhagen) amarish.sharma Danish Work Permit ( Person ID: 2913541) Sex : Male | Date of birth: 04/07/1980 | Nationality : Indian PROJECTS & ASSIGNMENTS PROFILE I am a biotech professional having over 6 years of research & academic experience. My experience highlights my passion, interest and strength in drug discovery & development program for diabetes, animal cell culture, Stem Cell Technology, in-vitro target focussed assay development & standardization and High Throughput Screening. I was also engaged in University teaching & research extensively. Multi-tasking, Can do attitude, risk taking ability, good communication skill and hard work are my strength. August 2010- October 2014 September’2009-April’2010 Assistant Professor Lovely Professional University, Punjab, INDIA | Research Scientist Biocon Ltd, Bangalore, India | Research Scientist March’ 2008-May’2009 August 2007- January 2008 Connexios Life Sciences, Bangalore, INDIA | Research Intern Nicholas Piramal Research Centre, Mumbai, India | PROFESSIONAL EXPERIENCE ▪ Assay development & Standardization for efficacy (Biosimilars/Bioequivalence studies). ▪ Toxicity studies (Toxicogenomics, ADME/Tox), its standardization/optimization. ▪ High-throughput screening (HTS) of NCE’s(Natural/Synthetic therapeutic molecules) using mammalian cell lines(L6,C2C12,H9C2,3T3-L1,3T3-A31,HepG2,HEK293,CHO-K1). ▪ Target focussed projects handled:  Standardization of the diabetic in-vitro assay model which deals with the comparative study of the efficacy of different Insulin analogues (Bio-similar), through assessing the percentage glucose drop using 3T3-L1 cell line.  Standardization of Mitogenic or cell proliferation assay model for the investigation of Mitogenic property of different Insulin analogues (Bio-similar) on induced diabetic model (hyperglycaemic condition) using 3T3-L1 cell line. Research & Development | Drug discovery program
  2. 2. PROFESSIONAL EDUCATION  Investigated the effect of Hepatocyte Nuclear Factor-4α phosphorylation and its transactivation and assessing AMPK activation in Hepatocytes on Type 2 Diabetes assay model.  Assessed the effect of cationic amphiphilic drugs induced Phospholipidosis and their in-vitro high throughput screening (HTS) in CHO-K1 cell line using the fluorescent-labelled phospholipid probe.  Standardized the bio-assay for the analysis of Nephrotoxicity (ADME/Tox) in HEK-293 cell lines and in-vitro screening of the NCE’s (New Chemical Entities) on induced Type 2 Diabetic assay model.  Investigated the relationship between IMTG (Intramuscular triglycerides) and Insulin resistance in Obese Type 2 Diabetic in-vitro model on C2C12 cell line followed by high throughput screening of new chemical entities for its respective efficacy.  Investigated the effect of HSD11b1 mediated Endoplasmic Reticulum stress (ADME/Tox) on Type 2 Diabetic invitro model using C2C12 cell line.  Studied the expression of CPT1A gene and ECHS1 gene which play a major role in accumulation of fat in the liver and is associated with insulin resistance and type 2 diabetes mellitus followed by high throughput in-vitro screening of new chemical entity for its efficacy.  University teaching of subjects namely Stem Cell Technology & Animal Biotechnology, Administrative responsibilities (Pedagogy development, Training, workshop & seminar organization).  Guided & co-ordinated graduate and post-graduate interns in project work concerned to Heavy metal Bioremediation and Food Bio-preservatives. POST-GRADUATE INTERNSHIP: August 2007 - January 2008 (6 months) Project title: ▪ “Bioactivity guided fractionation and in-vitro screening of MHCO2 isolated from marine bacteria showing Anti-Diabetic Activity ". ▪ Investigated the anti-hyperglycaemic effect of different plant metabolic extracts and extracts derived from marine source for their efficacy in glucose uptake in skeletal muscles through an in-vitro assay model using radio-labelled glucose and L6 cell line (skeletal muscles isolated from Rat),at Department of Biotechnology August 2006-December 2007 Master of Technology (M.Tech) in Biotechnology Bundelkhand University, INDIA July 2002 - July 2006 Bachelor of Technology (B.Tech) in Biotechnology Bundelkhand University, INDIA Research & Development | Drug discovery program
  3. 3. © European Union, 2002-2013 | Page 3 / 3  Expertise in Animal Cell Culture; Cell line Maintenance & Cryopreservation.  Cell line characterization and validation.  Assay development for efficacy (Biosimilars/Bioequivalence studies).  Toxicity studies (Toxicogenomics, ADME/Tox).  High-throughput screening (HTS).  RNA isolation, cDNA preparation and quantitative RT-PCR.  SDS-PAGE, Western blotting, and fluorescent imaging techniques.  Experience in working with radioisotopes.  Handled In-vivo experiments (Islets isolation, Pancreas, Mouse model).  Experience with data analysis and graphing software.  Interpret and report data to the drug discovery teams.  Prepare and maintain accurate laboratory records (SOPs).  Experience in handling HPLC and Mass Spectrometry.  TLC, Column Chromatography, End point Thermo cycler, Agarose gel electrophoresis. PERSONAL SKILLS Mother tongue Hindi Other language(s) UNDERSTANDING SPEAKING WRITING Listening Reading Spoken interaction Spoken production English C1/2 C1/2 C1/2 C1/2 C1/2 Replace with name of language certificate. Enter level if known. Danish A1/2 A1/2 A1/2 A1/2 A1/2 Replace with name of language certificate. Enter level if known. Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user Common European Framework of Reference for Languages Computer skills ▪ Good command of Microsoft Office™ tools ▪ Good command on statistical tools such as Graph pad prism & PLA, for analysing data. Presentations/Conferences Certifications Memberships  Poster Presentation- "Control of Cataract by increasing the activity of enzyme Polyol Dehydrogenase" : International Conference On Biomedical Research and Therapeutic Medicine, Bundelkhand University, India January 8, 2005;Authors: Amarish Sharma, Saurabh Verma  Poster presentation on “ELISA” in All India Engineering Project Contest, organized by Institute Of Engineering & Technology, Bundelkhand University, Jhansi, INDIA on March 21st, 2005.  Participated in National Sensitization Programme on Biotechnology held at D.D.U. Gorakhpur University, Gorakhpur, U.P, INDIA on 6th March, 2005.  Workshop on Chromatography & Mass Spectrometry-Certificate of Attendance ,Indian Institute of Chromatography and Mass Spectrometry LLP License 100108 March 2014  Summer Training at Post Graduate Department of Microbiology, King George Medical University,Lucknow-226003 License 1374/05 ; DOI: 07.09.05 September 2005  Faculty Development Programme on "Stem Cell Technology”, Lovely Professional University License 3652 June 2013  Faculty Development Programme on “Immunology and Immune-technology, Lovely Professional University License 1615 July 2011  MEMBER OF HELP AGE INTERNATIONAL CHARITABLE TRUST  MEMBER OF INDIAN SOCIETY FOR RADIATION BIOLOGY (Applied) Research & Development | Drug discovery program