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Goutam Mandal, Ph.D
No 5, 1st main, 1st Cross, Karnataka Layout,
2nd Stage, Basaveshwara Nagar, Bangalore 560079, Karnataka, India
Cell: 011 91 953 507 6402; Email: goutammandal77@gmail.com
An energetic and talented scientist with a proven track record of achieving targets on or before
time looking to accept new challenges to make differences in human life through knowledge
based discoveries
A.Position held:
1. Scientist – Cancyte Technologies Pvt. Ltd., Bangalore, India, June 2015 to date
2. Assistant Professor – Florida International University, Miami, USA, March, 2012 to May, 2015
3. Postdoctoral Research Associate – Florida International University, Miami, USA, March, 2009 to
May, 2012
4. Associate Scientist – Lupin Ltd., Pune, India, March, 2008 to March, 2009
5. Visiting Scholar – Wellcome Trust Biocentre, University of Dundee, UK, June 2007 – July 2007
B.Leadership experience highlights:
I. Industry:
a.Cancyte Technologies Pvt. Ltd. India (June 2015 to date): Scientist
1. Leading the project to build a human leukocyte antigen (HLA) registry with more than one million
registered donors
2. Leading the project to develop a novel uniplex PCR strategy for the diagnosis of multiple infectious
disease in a single PCR reaction
b. Lupin Ltd, Pune, India (April 2008 to March 2009): Associate Scientist
1. Successfully led the team to develop in vitro and in vivo assay methods for efficacy study of
biologics according to US, European and Indian Pharmacopeia
2. Planned and executed successfully to establish the first animal cell culture facility (Class 10,000)
for the Division of Biotechnology, Lupin Ltd.
II. Academics:
H. W. College of Medicine, Florida International University (March 2012 to May 2015): Assistant
Professor (Research Track)
1. Research: I won the competitive F.I.U. foundation grant of $50,000 as Principal Investigator for
the projects entitled – Leishmaniasis disease development: role of macrophages. The study
included (i) genome wide screening of essential genes for leishmaniasis disease development
and (ii) genome wide screening of essential genes for arsenic and antimony metabolism and
detoxification using CRISPR/Cas9 library
Ø Successfully amplified CRISPR/Cas9 library, packaged into the lentiviral vector system,
transduce into several cell lines and screened essential gene(s) for (i) leishmaniasis disease
development and (ii) arsenic and antimony metabolism and detoxification
2. Teaching
Ø Infectious diseases: Graduate and M.D program of H. W. College of Medicine
Ø Guided PhD students, undergraduate students and lab technicians for their regular research
progress, troubleshooting and lab safety training
3. Organizational responsibilities:
Ø Served in the Graduate Admission Committee (2012-2013, 2013-2014 and 2014-2015)

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Ø Served as a member of the internal grant review committee (2015)
Ø Committee member (publicity and logistics) for 3rd Annual International Conference on Tropical
Medicine, February, 2013, Florida International University, Miami, Florida, USA
Ø Lab manager
C. Team player experience highlights:
Postdoctoral Research Associate (April 2009 to February 2012), H. W. College of Medicine,
Florida International University
1. Lead researcher and co-researcher of several projects to explore the post-transcriptional and
post-translational gene regulation mechanisms of Leishmania drug facilitator Aquaglyceroporin
AQP1 using several cutting edge molecular and cell biology and biochemistry techniques
2. Lead researcher to develop biomarker for the early detection of environmental exposure of
arsenic
D. Publications
1. Book chapter:
Mandal G, Vaidya G, Sharma M, Bhatterjee H, Mukhopadhyay R. Drug resistance mechanisms of
Leishmania, In: Antimicrobial Drug Resistance; Springer (In press)
2. Research articles: Total 20; total citations: 591, h-index: 14 and i10 index: 16
(i) Mandal G, Mandal S, Sharma M, Charret KS, Papadopoulou B, Bhattacharjee H, Mukhopadhyay
R. Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation
of AQP1. PLoS Negl Trop Dis. 2015 Feb 25;9(2):e0003500
(ii) Mandal G, Sharma M, Kruse M, Sander-Juelch C, Munro LA, Wang Y, Vilg JV, Tamás MJ,
Bhattacharjee H, Wiese M, Mukhopadhyay R. Modulation of Leishmania major aquaglyceroporin
activity by a mitogen-activated protein kinase. Mol Microbiol. 2012;85:1204-18
(iii) Plourde M, Ubeda JM, Mandal G, Monte-Neto RL, Mukhopadhyay R, Ouellette M. Generation of
an aquaglyceroporin AQP1 null mutant in Leishmania major. Mol Biochem Parasitol. 2015;201:108-
11
(iv) Mukhopadhyay R, Mandal G, Atluri VS, Figarella K, Uzcategui NL, Zhou Y, Beitz E, Ajees AA,
Bhattacharjee H. The role of alanine 163 in solute permeability of Leishmania major
aquaglyceroporin LmAQP1. Mol Biochem Parasitol. 2011;175:83-90
(v) Wyllie S, Mandal G, Singh N, Sundar S, Fairlamb AH, Chatterjee M. Elevated levels of tryparedoxin
peroxidase in antimony unresponsive Leishmania donovani field isolates. Mol Biochem Parasitol.
2010;173:162-4
(vi) Mandal G, Saha P, Singh N, Sundar S, Chatterjee M. Functionality of drug efflux pumps in
antimonial resistant Leishmania donovani field isolates. Indian J Biochem Biophys. 2009;46:86-92
(vii)Mandal G, Wyllie S, Singh N, Sundar S, Fairlamb AH, Chatterjee M. Increased thiol levels protects
antimonial unresponsive Leishmania donovani field isolates against reactive oxygen species
generated by trivalent antimony. Parasitology. 2007;134:1679-87
3. Presentation: Lecture- 3; poster- 17
E. Technical expertise highlights
a. Cell biology:
• Performed cell proliferation/inhibition assays using reporter genes, flow cytometry, luciferase,
MTT, MTS and microscopic counting methods
• Analyzed apoptosis, cell cycle, drug transport activity and effects of drugs on several cellular
targets using multi-color flow cytometry
• Cell culture:

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i) Isolated and maintained mammalian primary cells for in vitro Leishmania infection and cytokine
studies
ii) Maintained and used multiple human and non-human immortal cell lines in several studies
iii) Isolated, maintained, and microinjected frog oocyte to study channel proteins
(aquaglyceroporins)
iv) Isolated, in-vitro propagated and maintained several species of Leishmania from infected
clinical and animal samples
b. Immunological assays:
• Performed immunophenotyping assays and estimated intracellular and secretory cytokines (using
bead array) of human and mouse leukocytes by multi-color flow cytometry
• Estimated several cytokines using ELISA
• Detected expression levels of several membrane and cytosolic proteins using western blotting
• Used immunofluorescence microscopy to study protein localization
c. Molecular Biology:
• Optimized PCR condition and typing HLA of donor samples (blood and saliva) using NGS
• Planned, designed primers and optimized PCR condition to develop viral diagnostic kit
• Amplified, packaged into the lentiviral vector system, transduced to screen CRISPR/Cas9 library
• Planned experiments, designed primers and probes and optimized conditions for PCR, RT-PCR,
RACE, quantitative PCR and ribonuclease protection assays for several studies
• Synthesized RNA (in vitro) to inject into frog oocytes
• Designed the strategy, cloned and sub-cloned, analyzed sequences of target genes for
homologous and heterologous expressions
• Performed site directed mutagenesis to study the structure-function relationship of proteins
• Used Beckman ceq 8000 DNA sequencer for Sanger sequencing
• Studied several genes of interest using southern and northern Blotting
• Used bioinformatic databases and tools to design several studies and data analysis
d. Animal handling:
• Handled and maintained (normal and parasite infected) several species of rodents
• Harvested normal and infected organs for patho-biological studies
• Used in vivo models to study (i) antileishmanial activity of plant derived compounds, (ii) protein
based therapeutics and, (iii) acute and chronic anti-inflammatory and analgesic activity and toxicity
of natural compounds
F. Education: Ph.D. (2008)- University of Calcutta, Institute of Post Graduate Medical Education
and Research, India
Thesis title: Antimonial resistance in Indian Leishmaniasis: role of Multi Drug Resistance (MDR)
phenotype
Summary: I have studied the role of trypanothione and its dependent antioxidant system and, drug
efflux pumps in the generation of antimonial resistance in Leishmania donovani clinical isolates. I
have established that elevated level of trypanothione is a major determining factor in antimonial
resistance in L. donovani clinical isolates. Faster rate of trypanothione biosynthesis and amplification
of thiol dependent antioxidant system protected resistant isolates from antimony induced thiol loss
and oxidative stress thereby generating antimonial resistance. Antimony resistant isolates also
showed higher activity of a non-classical MRP type pump.
G. Honours and Awards
2014: Winner H. W. College of Medicine, Florida International University, Pilot Fund -$50,000
2007: Fellowship from International Organization for Chemical Sciences in Development (IOCD)
to attend COST B22 Meeting, 10th – 13th June, 2007, Dundee, UK

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2007: Travel award from Council of Scientific and Industrial Research, Govt. of India, and Dept. of
Science and Technology, Govt. of India to attend COST B22 Meeting, 10th – 13th June,
2007, Dundee, UK
2005: P. C. Dandiya prize for best poster in XXXVIIth
Annual Conference of Indian Pharmacological
Society, January 14th-16th, 2005, Kolkata, India
2005 - 2008: Senior Research Fellow, Council of Scientific and Industrial Research, Govt. of India