2. Steroids are a man-made version of chemicals,
known as hormones, that are made naturally in
the human body.
Steroids are designed to act like these
hormones to reduce inflammation.
Steroids won’t cure your condition, but they’re
very good at reducing inflammation and will
ease symptoms such as swelling, pain and
stiffness.
3. In some conditions, such as rheumatoid
arthritis, the immune system produces
inflammation in the joints or other parts of
the body by mistake, which can cause
permanent damage if left untreated.
Steroids can be used to reduce this
immune reaction.
4. • Steroids are complex organic compounds as shown in figure.
• All the steroids possess the basic structure namely
cyclopentanoperhydrophenanthrene.
• Steroids as hormones (glucocorticoids, mineralocorticoids,
androgens, estrogens) perform a wide range of functions.
• They are very useful therapeutically.
5. • For instance, cortisone, due to its anti-inflammatory action is used in the
treatment of rheumatoid arthritis and skin diseases; derivatives of
progesterone and estrogens are employed as contraceptives.
• Certain derivatives of cortisone (e.g. prednisolone) are more effective in
their therapeutic action.
• Commercial production of steroids is very important.
• Cortisone was chemically synthesized, and this process involved as
many as 37 reactions. The cost of the so obtained product was around
$200/g (in 1950).
• With the introduction of biotransformation reactions, the number of
steps (microbial and chemical put together) was reduced to II, and cost
of the product was reduced to just $1/g in 1980! The credit obviously
goes to the developments in biotransformation.
6. • Deoxycholic acid → Cortisone, So cortisone
synthesis is an example of steroid biotransformation.
• The major difficulty in chemically synthesized
cortisone is need to introduce an oxygen atom at
number 11 position of the steroid ring which can be
accompanied by using microorganism.
• The fungus Cunningham Ella blakesleeana can
hydroxylate the steroid cortexolone to form
hydrocortisone with the introduction of oxygen at the
number 11 position.
7. • The fungus Rhizopus arrhizus for example
hydroxylates progesterone forming another steroid
with the introduction of oxygen at the number 11
position.
• Steroids are very important in pharmaceutical industry.
Steroid hormone regulates various aspect of
metabolism.
8. • A good example of steroid transformation processes involving
microorganisms like Rhizopus Nigerians which can be by
growing the mould in a fermentor using an appropriate growth
medium and incubation conditions to obtain high biomass, of
course, aeration and agitation are employed to achieve rapid
growth.
• After the growth of microorganism the steroid to be
transformed is added.
• When progesterone is added to a fermentor having Rhizopus
Nigerians which has been growing for about one day, the
steroid is hydroxylated at the number 11 position to form 11 – a
– hydroxyprogesterone.
• The product is recovered by extraction with methylene chloride
or various other solvents followed by purification
chromatographically and recovered by crystallization
9.
10. Production process of steroids:
• The production of steroids, entirely by biotransformation
reactions is not practicable. Therefore, microbial
transformation along with chemical reactions is carried out.
• The major steps involved in the biotransformation of
steroids are depicted in Fig.
• Stigma sterol extracted from soybeans or diosgenin isolated
from the roots of the Mexican barbasco plant can serve as
the starting material.
12. • Stigma sterol can be chemically converted to progesterone which is
subjected to biotransformation to form 11 α-hydroxyprogesterone by the
microorganism, Rhizopus nigricans. Cortisol (hydrocortisone), produced
from 11 α-hydroxyprogesterone by chemical reactions, undergoes
microbial transformation (organism-Corynebacterium simplex) to form
prednisolone.
• Further, cortisone formed from Cortisol can be subjected to
biotransformation by Corynebacterium simplex to produce prednisone.
When diosgenin is used as the starting compound, substance S can be
produced by chemical reactions which can be converted to Cortisol by
biotransformation with the help of the microorganism Curvularia lunata.
• Biotransformation of steroids is usually carried out by batch fermentation.
Use of immobilized cells or immobilized enzymes is gaining importance
in recent years. This is advantageous since the biotransformation is more
efficient with high substrate concentration, short conversion time and good
product recovery.
13. • Since the steroids are not water soluble, the microbial
transformation reactions have to be carried out in organic solvent
(water-immiscible) system. However, the organic solvents are
toxic to micro-organisms or enzymes. It is ideal to use an aqueous
two phase system for biotransformation of steroids.
14. Advantages of Steroids:
• Steroids can be used to treat a wide range of
conditions, including: asthma and chronic obstructive
pulmonary disease (COPD)
• hay fever (Rhinitis)
• hives and eczema
• painful joints or muscles – such as arthritis, tennis
elbow and frozen shoulder pain caused by an irritated
or trapped nerve – such as sciatica
• Inflammatory bowel disease – such as Crohn's disease
& Lupus multiple sclerosis (MS)
15. Side effects of steroids
• Steroids do not tend to cause significant side effects if they're
taken for a short time or at a low dose. But sometimes they
can cause unpleasant side effects, such as an increased
appetite, mood changes and difficulty sleeping. This is most
common with steroid tablets.
• The side effects will usually pass once you finish the
treatment, but do not stop taking your medicine without
speaking to your doctor. Stopping a prescribed course of
medicine can cause further unpleasant side effects
(withdrawal symptoms).