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Histology of testis &
epididymis,
Histology of testis
• Testis is covered by
---tunica vaginalis
----tunica albuginea
----tunica vasculosa
• Tunica albuginea- is a thick white fibrous connective tissue—called
capsule of testis
• Deep to the capsule ,, there is tunica vasculosa– which is a highly
vascularised connective tissue.
• Septa from tunica albuginea divide testis into 200 to 300 lobules
• Each lobule contains 1- 4 seminiferous tubule
• Between the seminiferous tubules ,, there is loose connective
tissue k/a interstitial tissue & blood vessels
lobule
seminiferous
tubule
straight tubule
Rete testis
Efferent
ductules
head of
epididymis
septa
Thus---
• Glandular portion of testis consist of –
1 seminiferous tubule-
a> Spermatogenic cells
b> supporting cell of sertoli –
also K/A sustentacular cell.
2 Interstitial tissue
seminiferous
tubule
interstitial
tissue
T.s. of
seminiferous
tubule
seminiferous tubule-
• Sperm producing tubule
• Convoluted and long
• About 30-70 cm long & 200 micro-metre in
diameter
• Surrounded by connective tissue [lamina
propria]----consists of flattened or spindle
shaped myoid cells
• Myoid cells are contractile in nature & help
spermatozoa to move through seminiferous
tubules.
......seminiferous tubule-
• Tubule is lined by stratified cuboidal epithelium
• Tubule contains--------
i) ----spermatogenic cells
[ in different stages of spermatogenesis]
ii) ---Supporting cells of sertoli
spermatogenic cells
Spermatogenic cells in 4
successive stage—
--spermatogonia,
--primary spermatocyte
--secondary
spermatocyte
---spermatids.
• --primary spermatocyte
are the largest cells
seminiferous tubule…
it contains----
Spermatogenic cells in 4
successive stage—
--spermatogonia,
--primary spermatocyte
--secondary spermatocyte
---spermatids.
supporting cell of sertoli
* supporting cell of
sertoli – also K/A
sustentacular cell.
*Tall columnar cell
* Its lateral process
form a tight junction
k/a BLOOD TESTIS
BARRIER
protects secondary
spermatocyte,
spermatids &
spermatozoa from
immune attack
BLOOD TESTES BARRIER
BLOOD TESTIS BARRIER
--secondary spermatocyte
BLOOD TESTIS BARRIER
Function of sertoli cells
• Provide mechanical support
• Provide nutriton to Spermatogenic cells
• Form blood testis barrier
• Secrete androgen binding protein
• Synthesize antimullerian hormone
• Do phagocytosis of residual body,, degenerating
Spermatogenic cells
Interstitial tissue
• occupies space b/w the
seminiferous tubules
• consist of
-- Loose connective tissue &
-- interstitial cells of Leydig
(OVAL CELL).
• It forms endocrine part of
testis
• Secrete testosterone & a
little estrogen
low power /T.S. of
testis tunica
albuginea
tunica vasculosa
seminiferous tubule
spermatogenic cells
&
Sertoli cell
Leydig cells
septa
low power /T.S. of testis [nmc slide]
epididymis
. Highly coiled
tubular structure
pathway of sperm from seminiferous
tubule
seminiferous tubules
[highly coiled] in each
lobule
join to form 20 to30
straight tubules
they anastomose to form
network of tubules
called the RETE TESTIS
gives rise to 12to30
EFFERENT DUCTULES
which emerge near the
upper pole
and enter the Epididymis
epididymis
1. Highly coiled muscular
tubule
2. 6 meter long, made up
of smooth muscle
3. smooth muscle arranged
in circular pattern
4. is lined by
pseudostratified
columnar epithelium
with stereocilia.[non-
motile cilia]
5. Can store sperms for
several months
6. Continues as ductus
(vas) deferens
smooth
muscle pseudostratifi
ed columnar
epithelium
stereocilia
sperm
in lumen
T.S. OF epididymis
connective
tissue
Epididymis-----[ nmc slide]
epididymis stereocilia
In histo
practical
class
low power /T.S. of
testis
tunica
albuginea
tunica vasculosa
seminiferous tubule
spermatogenic cells
&
Sertoli cell
Leydig cells
septa
TESTIS
TESTIS
seminiferous tubule….
• b> supporting cell of
sertoli – also K/A
sustentacular cell.
• Tall columnar cell
• Its lateral process form
a tight junction k/a
BLOOD TESTES BARRIER
protects secondary
spermatocyte, spermatids &
spermatozoa from immune
attack
epididymis
1. Highly coiled mucular
tubule
2. 6 meter long, made up
of smooth muscle
3. smooth muscle arranged
in circular pattern
4. is lined by
pseudostratified
columnar epithelium
with stereocilia.[non-
motile cilia]
5. Can store sperms for
several months
6. Continues as ductus
(vas) deferens
smooth
muscle pseudostratifi
ed columnar
epithelium
stereocilia
sperm
in lumen
T.S. OF epididymis
connective
tissue
epididymis
epididymis stereocilia
ductus deferens / (vas deferens)
muscle coat
PROSTATE
fibromuscula
septa
Prostatic follicle /
gland
Smooth muscle
Simple Columnar
epithelium
amyloid body or
corpora amylacea
PROSTATE
ureter
ureter
Copyright © McGraw-Hill CompaniesFigure 21-17
T.S. OF PENIS
T.S. OF PENIS
seminal
vesicle
Location on
posterior
surface of
bladder
In cut section
exhibits honey
comb like pattern
Seminal
vesicles
Seminal vesiscle
The end
• Deep to lamina propria there is basal lamina
MALE REPRODUCTIVE SYSTEM
SERTOLI
CELLS
SPERMATOGONIA
1º SPERMATOCYTE
2º SPERMATOCYTE
SPERMATIDS
 SPERMATOGENESIS
SPERMATOGONIA 1º SPERMATOCYTE 2º SPERMATOCYTE SPERMATIDS
SERTOLI CELLS:
- columnar with adjoining lateral processes
- Sertoli-Sertoli junctions divide seminiferous
tubules into basal and adluminal
compartments
- extend from basal lamina to lumen
Copyright © McGraw-Hill CompaniesFigure 21-5
epididymis

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Histology of testes & epididymis

  • 1. Histology of testis & epididymis,
  • 2. Histology of testis • Testis is covered by ---tunica vaginalis ----tunica albuginea ----tunica vasculosa • Tunica albuginea- is a thick white fibrous connective tissue—called capsule of testis • Deep to the capsule ,, there is tunica vasculosa– which is a highly vascularised connective tissue. • Septa from tunica albuginea divide testis into 200 to 300 lobules • Each lobule contains 1- 4 seminiferous tubule • Between the seminiferous tubules ,, there is loose connective tissue k/a interstitial tissue & blood vessels
  • 4. Thus--- • Glandular portion of testis consist of – 1 seminiferous tubule- a> Spermatogenic cells b> supporting cell of sertoli – also K/A sustentacular cell. 2 Interstitial tissue
  • 6. seminiferous tubule- • Sperm producing tubule • Convoluted and long • About 30-70 cm long & 200 micro-metre in diameter • Surrounded by connective tissue [lamina propria]----consists of flattened or spindle shaped myoid cells • Myoid cells are contractile in nature & help spermatozoa to move through seminiferous tubules.
  • 7. ......seminiferous tubule- • Tubule is lined by stratified cuboidal epithelium • Tubule contains-------- i) ----spermatogenic cells [ in different stages of spermatogenesis] ii) ---Supporting cells of sertoli
  • 8. spermatogenic cells Spermatogenic cells in 4 successive stage— --spermatogonia, --primary spermatocyte --secondary spermatocyte ---spermatids. • --primary spermatocyte are the largest cells
  • 9. seminiferous tubule… it contains---- Spermatogenic cells in 4 successive stage— --spermatogonia, --primary spermatocyte --secondary spermatocyte ---spermatids.
  • 10. supporting cell of sertoli * supporting cell of sertoli – also K/A sustentacular cell. *Tall columnar cell * Its lateral process form a tight junction k/a BLOOD TESTIS BARRIER protects secondary spermatocyte, spermatids & spermatozoa from immune attack
  • 11. BLOOD TESTES BARRIER BLOOD TESTIS BARRIER --secondary spermatocyte BLOOD TESTIS BARRIER
  • 12. Function of sertoli cells • Provide mechanical support • Provide nutriton to Spermatogenic cells • Form blood testis barrier • Secrete androgen binding protein • Synthesize antimullerian hormone • Do phagocytosis of residual body,, degenerating Spermatogenic cells
  • 13. Interstitial tissue • occupies space b/w the seminiferous tubules • consist of -- Loose connective tissue & -- interstitial cells of Leydig (OVAL CELL). • It forms endocrine part of testis • Secrete testosterone & a little estrogen
  • 14. low power /T.S. of testis tunica albuginea tunica vasculosa seminiferous tubule spermatogenic cells & Sertoli cell Leydig cells septa
  • 15. low power /T.S. of testis [nmc slide]
  • 17. pathway of sperm from seminiferous tubule seminiferous tubules [highly coiled] in each lobule join to form 20 to30 straight tubules they anastomose to form network of tubules called the RETE TESTIS gives rise to 12to30 EFFERENT DUCTULES which emerge near the upper pole and enter the Epididymis
  • 18. epididymis 1. Highly coiled muscular tubule 2. 6 meter long, made up of smooth muscle 3. smooth muscle arranged in circular pattern 4. is lined by pseudostratified columnar epithelium with stereocilia.[non- motile cilia] 5. Can store sperms for several months 6. Continues as ductus (vas) deferens smooth muscle pseudostratifi ed columnar epithelium stereocilia sperm in lumen T.S. OF epididymis connective tissue
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  • 28. low power /T.S. of testis tunica albuginea tunica vasculosa seminiferous tubule spermatogenic cells & Sertoli cell Leydig cells septa TESTIS
  • 30. seminiferous tubule…. • b> supporting cell of sertoli – also K/A sustentacular cell. • Tall columnar cell • Its lateral process form a tight junction k/a BLOOD TESTES BARRIER protects secondary spermatocyte, spermatids & spermatozoa from immune attack
  • 31. epididymis 1. Highly coiled mucular tubule 2. 6 meter long, made up of smooth muscle 3. smooth muscle arranged in circular pattern 4. is lined by pseudostratified columnar epithelium with stereocilia.[non- motile cilia] 5. Can store sperms for several months 6. Continues as ductus (vas) deferens smooth muscle pseudostratifi ed columnar epithelium stereocilia sperm in lumen T.S. OF epididymis connective tissue
  • 34. ductus deferens / (vas deferens) muscle coat
  • 36. fibromuscula septa Prostatic follicle / gland Smooth muscle Simple Columnar epithelium amyloid body or corpora amylacea PROSTATE
  • 39. Copyright © McGraw-Hill CompaniesFigure 21-17 T.S. OF PENIS
  • 41. seminal vesicle Location on posterior surface of bladder In cut section exhibits honey comb like pattern Seminal vesicles
  • 44. • Deep to lamina propria there is basal lamina
  • 45. MALE REPRODUCTIVE SYSTEM SERTOLI CELLS SPERMATOGONIA 1º SPERMATOCYTE 2º SPERMATOCYTE SPERMATIDS  SPERMATOGENESIS SPERMATOGONIA 1º SPERMATOCYTE 2º SPERMATOCYTE SPERMATIDS SERTOLI CELLS: - columnar with adjoining lateral processes - Sertoli-Sertoli junctions divide seminiferous tubules into basal and adluminal compartments - extend from basal lamina to lumen
  • 46. Copyright © McGraw-Hill CompaniesFigure 21-5
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  • 48.