Reza Khorramirouz

1. Evaluating the role of autologous mesenchymal stem
cell seeded on decellularized pericardium in the
treatment of myocardial infarction: an animal study
A M Kajbafzadeh, S H Ahmadi Tafti*, R Khorramirouz, M Kameli, S Sabetkish, S
Rabbani, M J Mohseni .Pediatric Urology Research Center, Section of Tissue
Engineering and Stem Cell Therapy, Pediatric Center of Excellence, Tehran University
of Medical Sciences, Iran (IRI)*Animal Research Units, Tehran Heart Center, Tehran
University of Medical Sciences, Tehran, Iran (IRI)
INTRODUCTION
Inappropriate left ventricular remodeling following
myocardial infarction (MI) can result in subsequent severe
cardiac dysfunction. Fibrotic remodeling, scar formation, and
collagen disposition are key participants for repair of
infarcted myocardium . Here, we tested the hypothesis that
decellularized pericardium (DP) or seeded pericardial patch
with autologous adipose-derived mesenchymal stem cells
(ADMSCs) can be safely used in a MI scar and improve heart
function.
METHODS
Sixteen rabbits were randomly divided into four equal
groups. The control group included rabbits without MI
induction (G1). Four weeks after MI induction by ligation
of the left anterior descending artery in other 12 rabbits (3
equal groups), animals of G2 received DP patch with
labeled ADMSCs. DP patch was implanted in animals of
G3. Rabbits in G4 remained without any intervention after
MI induction. Serial examinations including
echocardiography, scanning electron microscopy,
histology and immunohistochemistry (IHC) were
performed to evaluate the efficacy of the implanted
scaffolds on recovery of the infracted myocardium.
RESULTS
The results demonstrated that left ventricular contractile
function and myocardial pathological changes were
significantly improved in rabbits implanted with either
DP or ADMSC-seeded pericardium. However, the
seeded Pericardium was more effective in scar repairing.
IHC staining with Desmin and CD34 after 2 months of
operation and positive immunofluorescence staining
verified the differentiation of ADMSCs to functional
cardiomyocytes.
DISCUSSION & CONCLUSIONS
The ability of DP seeded with autologous ADMSC to
repair MI remains controversial. Taken together, we can
conclude that the application of pericardial patch seeded
with autologous ADMSC can play an active role in
treatment of MI and may prove a desirable approach to
enhance cell engraftment. This novel method may gain
vigorous momentum over other presented techniques for
repair of MI. Based on the results of this study; DP
seeded with autologous ADMSC may advance via
animal trials into a clinical setting addressing patients
with MI.
Fig.3. Hematoxylin & eosin, trichrome staining,
immunohistochemistry and immunofluorescent of
decellularized patch seeded with adipose derived
mesenchymal stem cell after 1 month post-operation.
Fig.2. Hematoxylin & eosin, trichrome staining and
immunohistochemistry of decellularized patch
implantation after 1 month post-operation.
Fig.4. Surgical method
Fig.1. Scanning Electron Microscopy of control & acellular