All the process in DNA replication in bacteria .

1. MEWAR UNIVERSITY
PRESENTATION BY:-
rambhagat mandal
Bsc.Ag 1st year
MUR 1500210
SUBMITTED TO :- Mr. SAURABH SAXENA
PRESENTATION TOPIC :- DNA REPLICATION

2. DNA REPLICATION IN BACTERIA
CELL
 DNA replication is complete in three steps :-
1.INITIATION
2.ELONGATION
3.TERMINATION
*Semiconservative:-When replication is starts then
double stranded DNA become half of parential
molecule and half of new molecule is known as
semiconservatie.

3. 1.INITIATION
 There are many enzymes involve.
1.Primase:-Makes RNA primers from DNA template.
2.DNA polymerase:-Synthesis of DNA
3.Topoisomerase:-Relaxes super coiling ahead of
replication fork.
4.Helicase:-unwind and seprate d.s.DNA.
5.DNA ligase:-Makes covalent bond to join okazaki
fragments and repair it.

4. 7.Single strand binding proteins:-Synthesis of new strand.
8.Transposase:-Cuts sugar of DNA leaving single strand’sticky
ends’.

5. 2.ELONGATION
 Synthesis of DNA in each old strand which synthesis
of new complementry strand.There are many proteins
are invoved in protein synthesis .
 DNA polymerase 3 remove mismach nucleotide and
put correct nucleotide.

6. 3.TERMINATION
 After 40 minutes two replication fork meet,two inter
wined DNA molecules one seperated by other
enzymes sugaranteeing that each daughter cell will
inherit one complete chromosome after binary fission.
 A)LEADING STRAND SYNTHESIS:-
 DNA polymerase read the template in the 3’-5’
direction ,bringging in triphosphate nucleotide that
hydrogen bond with their coplement in the template
strand.

7.  Triphosphate nucleotides provide the DNA polymerase to
covalently join nucleotides into the continuous strand
forming and elongating chain of nucleotides from 5’-3’.
 B)LAGGING STRAND SYNTHESIS:-
 DNA polymerase moves away from the replication fork and
discontiuous process starts and stop occurs with the new
strand always lagging behind the leading strand is called as
lagging strand (okazaki fragments).
 DNA ligase join okazki fragments into a complete and
elongating single strand.