In this slide contains instrumentation of Fourier-Transform Nuclear Magnetic Resonance (FT-NMR). Presented by: P. Venkatesh. (Department of pharmaceutical analysis). RIPER, anantpur.

1. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 1
A Seminar as a part of curricular requirement
for I year M. Pharm I semester.
Presented by
Mr P.Venkatesh.
(Reg. No. 20L81S0714)
Under the guidance of
Dr. P.Ramalingam sir., Ph.D
Directer- R&D division
Professor of pharmaceutical analysis
and medicinal chemistry
President-IPA local branch Anantapuram.

2. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 2
• Introduction
• Fourier transformation
• Components of FT-NMR
• Instrumentation
• Advantage
• Reference
Contents

3. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 3
• NMR Nuclear magnetic resonance.
• In this effect of magnetic field on nuclei are studied.
• For example we will take ethane molecule.
Introduction

4. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 4
Introduction
• The proton gives NMR signals when we apply
magnetic field externally.

5. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 5
Introduction

6. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 6
• Larmor equation :
• Gyromagnetic magnetic ratio depends on magnetic
movement and angular movementum.
Introduction

7. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 7
Introduction
•Criteria required for NMR signal
• Nuclei should have spin quantum number.
I=1/2
• We should apply external magnetic field.
• We should apply energy in the form of EMR that
resonates with energy gap
Radiowaves

8. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 8
FOURIER TRANSFORMER
• Mathematical operations inwhich
Complex wave forms
Simple mathematical
operations

9. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 9
FOURIER TRANSFORMER
• Mathematical operations required to convert time
domine spectrum to frequency domine spectrum
followed by S/N ratio .
• Digital data must be transformed to frequency data

10. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 10
FOURIER TRANSFORMER
• A computer is essential to solve these complex
equations.

11. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 11
Continuous wave nuclear magnetic resonance.
(CW-NMR)
Types of NMR

12. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 12
• Fourier transformer nuclear magnetic resonance
(FT-NMR)
Types of NMR

13. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 13
• Frequency synthesizer.
• Pulse switch.
• Radio frequency transmitter.
• Magnets.
• Sample holder.
• Radio frequency amplifier.
• Phase sensitive detector.
• Time swept computer
• Fourier transformer
Components of FT-NMR

14. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 14
INSTRUMENTATION

15. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 15
• Place the sample in sample holder and the sample should
be in the form of solution.
• If we take proton NMR,in that condition the sample must
contain proton.
• Now we will generate a radio frequency in the form of
MHz.
• This radio frequency is connected to pulse switch.
• Due to RF passage through the sample all the protons
alline in the direction of radio frequency.
INSTRUMENTATION

16. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 16
• As the magnets present in both the side of the sample will
generate magnetic field.
• These magnetic field is controlled by swap generator.
• If increase in magnetic field resolution will be better.
• Now at these specific conditions when the magnetic field
exceeds the strength of radio frequency
• Protons will Aline in the direction of magnetic field.
• This is known as resonance.
• Resonance in the protons takes place.
INSTRUMENTATION

17. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 17
• Receiver coil will receive the signal to wards amplifier.
• Amplifier will amplifies these signals finally detector will
detect these signals.
• Finally Fourier transformer converts time domine
spectrum to frequency domine spectrum.
INSTRUMENTATION

18. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 18
• FT-NMR is more sensitive that can measure weak signals
• Has wide spread applications espically for carbon NMR,
proton NMR giving high signal to noise ratio
Facilitate rapid scanning
• Can be obtained with less than 0.5 mg of compound.
• Used in industrial quality control.
• MRI is the most prominent FT-NMR application.
ADVANTAGES OF FT-NMR

19. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 19
1. H.kaur. Instrument method of chemical analysis 4th
edition.2010,page number421-446.
2. Skoog, Douglas A. Principles of instrumental
analysis.Educ.1972.49,6,A362.
3. H.H.Willard,Merritt.Instrumental method of analysis, 7th
edition.1998.
4. Dr.Puspendra kumar. Pharmaceutical analysis.2018.
REFERENCES

20. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 20