What is culture media Bacteria culture Importance of culturing. Culturing and medium. History of culture media. How many types of growth media . Basic components of culture media. Classification Consistancy Nutritional components Functional use Aseptic condittion . General steps for preparation of culture media . Selective media . Enrichment media. Storage of culture media.

1. Selective and
enrichment media

2. What is culture media
Bacteria culture
Importance of culturing.
Culturing and medium.
History of culture media.
How many types of growth media .
Basic components of culture media.
Classification
Consistancy
Nutritional components
Functional use
Aseptic condittion .
General steps for preparation of culture media .
Selective media .
Enrichment media.
Storage of culture media.

3. An artificial culture media must provide
environment and nutional condition.
A culture media contains water,nutritional
elemennts and some growth factor.
The ph of the medium must be set accordingly.
Uses=
Enrich the number of bacteria.
Select for certain and supress other.
Differentiate among different kinds of bacteria.

4. A Microbial culture is a method of multipying
microorganism.
Microbial culture are used to determine the type of
organism,its abundace in the sample being tested or
both.
MAIN PURPOSE
Isolation of bacteria.
To create antigen for laboratory.
Culture bacteria for genetic study.

5.  CULTURE= It is term given to microorganism
that are cultivate in the lab for the purpose of
identification and studying.
 MEDIUM= IT is the term given to the
combination of ingredients that will support
the growth and cultivation of microorganism
by providing all the essential nutrient required
for the growth.

6.  Before the use of agar, attempts were made to
use gelatin as solidifying agent, gelatin had
some inherent problems .
 It existed as liquid at normal incubating
temprature 35-37◦.
 HOW MANY TYPES OF GROWTH MEDIA=
 There are two major types of grwoth media-
 A= Cell culture
 B= Microbiology culture

7.  Nutrients
-Energy source
-carbon source
-nitrogen source
 Mineral salts
 A suitable physical factor = temperature

8.  BACTERIA culture media can be divided in at
least three ways
 A=Conistency
 B=Nutritional components
 C= Functional use

9. LIQUID MEDIA = There are available for use in
test tube etc.
Liquid media are some times referred as
BROTHS.
SOLID MEDIA= Agar is most commomly used.
SEMI SOLID= Used in demonstrating bacterial
mortility.

10. SIMPLE MEDIA = simple media such as pepton
water, nutrient agar can support non fastidious
bacteria.
COMPLEX MEDIA= Complex media such as
blood agar have ingredients whose exact
components are difficult to estimate.
SYNTHETHIS MEDIA= Especially prepared
media for reseach purpose where the
composition is well known.

11.  BASAL MEDIA
 ENRICHED MEDIA
 SELECTIVE MEDIA
 ENRICHMENT MEDIA
 DIFFRENTIAL MEDIA
 TRANSPORT MEDIA
 ANAEROBIC MEDIA

12.  Sterile equipment and media are used during
bacteria culture.
 Culture media and culture tubes are open for
minimum time duration.
 All work done near the burner in laminar air
flow banch.

13.  Weight the media and mix in distilled water.
 Now transfer the the media in autoclave for 20
to 25 min at 15psi.
 Now inoculation is done.
 Incubate in cubator for 24 hours for colony
growth.

14.  They are designed to inhibit unwanted
commensal or contaminating bacteria.
 Selective media are agar based.

15.  EMB [ EOSIN METHYLENE BLUE AGAR] It
is selective for gram negative bacteria .
 Composition of EMB
S.NO. COMPOUND VOL. IN 1000 ML
1 Peptone 10 gm
2 Lactose 10 gm
3 Potassium diphosphate 2 gm
4 Agar 15 gm
5 Eosin 4 .4 gm
6 Methylene blue .065 gm

16.  PHENYLETHYL ALCOHOL AGAR [Used for
the isolation of gram positive bacteria]
 CHEMICAL COMPOSITION
S.NO
.
COMPOUND VOL. IN 1000 ML
1 Casein enzymic
hydrolysal
15 gm
2 Soya peptone 5 gm
3 Sodium chloride 5 gm
4 Phenyl alcohol 2.50 gm
5 Agar 15 gm

17.  This media allow metabolically fastidious
microorganism to grow because the addition of
specific growth factor.
 It is same as selective media , but enrichmnet
media is liquid in consistency.

18.  It is used to isolate SALMONELLA Species.
 COMPOSITION
s.no. COMPOUND VOLUME IN 1000 ML
1 Casein enzymic hydrolysate 5 gm
2 lactose 4 gm
3 Sodium phosphate 10 gm
4 Sodium hydrogen selenit 4 gm
SELENITE F BROTH

19.  LACTOSE BROTH[ FOR detection of
COLIFORMS and SALMONELLA.
 COMPOSITION
S.NO. COMPUND VOL. IN 1000 ML
1 Peptone 5 gm
2 Beef extract 3 gm
3 lactose 5 g,

20.  STORAGE OF CULTURE
MEDIA
 Prepared media in individual screw capped
bottles can be stored for weeks at room
temperature .