We are currently assessing two isothermal technologies, recombinase polymerase amplification (RPA) and loop mediated isothermal amplification (LAMP), for the detection of Candidatus Liberibacter solanacearum (CLso), the putative causal agent of Zebra Chip disease of potato in field samples. This bacterium is unculturable, its’ genome has a low GC content, and it is present in host potato plants in low titre and non-uniform distribution. These are all challenges that need to be addressed when designing and optimising the in-field diagnostic for CLso.

1. SESSION 3: FAST SCIENCE
In-field diagnostics for Ca. L.
solanacearum (CLso)
Rebekah Frampton

2. In-field diagnostics for Ca. L. solanacearum (CLso)
SCRI 2014 calendar
CLso genome
 1.3 Mb
 35% GC
 Whole genome
comparisons found CLso
specific targets
NZ GrowerRecombinase polymerase
amplification (RPA)
 Isothermal
 Quick
 Resilient
 Sensitive
0
500
1000
1500
2000
2500
3000
2 4 6 8 10 12 14 16 18
RelativeFluorescent
Units
Time (min)
10000
00
10000
0
Copy number