Monitoring of Toxin-Producing Cyanobacteria

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Monitoring of Toxin-Producing Cyanobacteria in Littoral Regions of the Muskingum River Watershed, Ohio.
Presented at the Ohio Academy of Sciences, 2012.

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Monitoring of Toxin-Producing Cyanobacteria

  1. 1. Monitoring of Toxin-ProducingCyanobacteria in Littoral Regions of the Muskingum River Watershed, Ohio A.D. Minerovic1, K.C. Scotese1, J.R. Beaver1, R.D. Davic2, and D.M. Oleskiewicz2 1BSA Environmental Services, Inc. 2Ohio Lake Management Society
  2. 2. The Muskingum River Watershed • Largest watershed in Ohio – Over 8,000 sq. mi. – 16 reservoirs • Ohio Lake Management Society – CLAM program2
  3. 3. Ohio Level III Ecoregions Charles Mill Atwood Leesville Pleasant Hill Tappan Clendening Wills Creek Piedmont Seneca Eastern Corn Belt Plains Erie Drift Plain Western Allegheny Plateau3
  4. 4. Percent Land Use FORESTED AGRICULTURAL OTHER 100 90 80 70 60 Percent 50 40 30 20 10 0 WESTERN ALLEGHENY PLATEAU ERIE DRIFT PLAIN4
  5. 5. Project Objectives • Examine littoral phytoplankton communities in different ecoregions • Relationship between N-fixing cyanobacteria, land use patterns, and hydrologic data5
  6. 6. Methods • Collected from littoral regions – July-October 2011 • 62 whole water samples – Phytoplankton – Algal toxins • Hydrologic Data • Data analysis – PRIMER 6 Seneca Lake algal bloom sample Photo courtesy of Heather Raymond, Ohio EPA6
  7. 7. 7
  8. 8. Cylindrospermopsis raciborskii • Capable of fixing nitrogen • Capable of Cylindrospermopsis raciborskii producing algal toxins • Forested reservoirs Tappan Lake, Cylindrospermopsis bloom Photo courtesy of Heather Raymond, Ohio EPA8
  9. 9. R² = 0.71 Proportional Biovolume by Site 100% 250 90% 80% 200 Mean Water Residence Time (Days) Percent Biovolume 70% 60% 150 50% 40% 100 30% 20% 50 10% 0% 0 N-FIXING CYANO NON N-FIXING CYANO OTHER MEAN WRT9
  10. 10. ELISA • Immunoassay sensitive to specific toxins • Different levels of detection and quantification –Microcystin –Cylindrospermopsin10
  11. 11. Microcystin Results • n=62 Below Limit of Detection <0.10 ppb 7 Below Limit of Quantification <0.15 ppb 38 Quantifiable >0.15 ppb 17 • No samples >6.0 ppb (Ohio EPA Public Health Advisory Threshold)11
  12. 12. Cylindrospermopsin Results • n=41 Below Limit of Detection <0.05 ppb 26 Below Limit of Quantification <0.10 ppb 13 Quantifiable >0.10 ppb 2 • No samples >5.0 ppb (Ohio EPA Public Health Advisory Threshold)12
  13. 13. Conclusions • Results indicate a link between land use patterns, WRT, and phytoplankton community composition – Limited data, not contemporaneous • Littoral samples • Nutrient data collected at different times • Future studies13
  14. 14. Acknowledgements • Muskingum Watershed Conservancy District • Ohio Lake Management Society Lake Keepers • US Army Corps of Engineers14

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