PROM an organic source of chemical DAP

1. Nirmal kumar
M.Ttech (biotechnology)
R.B.S Engg. Tech . Campus
Bichpuri agra
Project Guide:-
Dr. V. K Srivastava
HOD , R.B.S engg. Tech . Campus
Bichpuri Agra

2. introduction:-
 What is PROM?
 Benefits of PROM
 PROM manufacturing process
 Specification of PROM
 Different forms of PROM
 Objectives
 Material and method
 Identification of bacterias

3. Phosphate Rich Organic Manure (PROM) is a green chemistry
of phosphate fertilizer.
 A value-added product produced by co-compositing various
organic wastes with high grade rock phosphate in fine size.
A variety of organic materials such as FYM, vermicompost
dung, crop wastes, press mud from sugar industry, solid
wastes from fruit juice industry, oil cakes.
Ministry of Agriculture and Cooperation has now pproved
PROM as fertilizer in 2012 under Fertilizer Control Order
(FCO)

4. The world today consumes around 150 million tones of
high grade phosphate mineral, 90% of which goes into the
production of chemical phosphatic fertilizers.
Government of India used to spend substantial amount as
subsidy to make DAP, MAP and SSP available to Indian
farmers at affordable costs
During 2010 and 2011 the expenditure of subsidy on DAP
sky rocketed to ‘120,000 corer of rupees. This trend
continues in one form or the other. Today 50Kg of DAP costs
around Rs 1,273/‐ despite heavy subsidy which used to be Rs
600/‐ around the year.

5.  Acts as alternative to DAP and makes soil soft and
enriched with nutrients for long time
 Provides phosphorus to the second crop planted in a
treated area as efficiently as the first
 Can be produced using acidic waste solids recovered
from the discharge of biogas plants, thus makes
environment sound
 Due to organic manure presence, leaching and runoff is
prevented
 Increase the soil water holding capacity.

6. 1. Collect FYM/vermicompost/anaerobic digest sludge
(minimum 2-3 month old )And rock phosphate
2. Blend the material – particle size less then 4 mm
(operating temp 30-350c optimum, 600 maximum)
3. Microbial culture used bacillus megatherium var
phosphaticum for phosphate solublising size of
inoculum 3%-5% Mixed in bioreactor for 7-10 days
4. Azotobactor microbes culture 3-7 % inoculums use for
nitrogen fixing in soil. (This setp is also take 5-10 days )
5. After all this activity we pack fertilizer in required bag
and store in dry place

7. PROM powder form

8. PROM granules form

9. PROM as per FCO are as follows:
[1] Particle Size = 90% passing through 4 mm
[2] Total organic carbon = minimum 7.9%
[3] Total Nitrogen as N = 0.4%
[4] Total P2O5 = 10.42%
[5] C:N ratio = less than 20:1
[6] pH of solution = maximum 6.7
[7] conductivity = 8.2
[8] Moisture maximum = 25%
[9] Bulk Density (g/cm3) = less than 1.6

10. 0
500
1000
1500
2000
2500
1 2 3 4 5 6 7 8
different land
control
PROM
Different land
y
i
e
l
d

11. objective
 “To isolate the PROM producing bacteria and
process optimization ”

12. Azotobactor bacteria
Size -1.5-2.0μm
Gram negative
White in colour ,round
shape,chain forming,
Aerobic,
Growth temp-28±3
Growth time 4-7 days

13. Bacillus megatherium
Cell morphology-
Rod shape,dimeter 5-7 µm
,cell length -10 µm,Motile,
gram positive
Colony morphology –
circuler,entire,opaque,white
Growth condition-
temp- 320c optimum,pH-7
Growth condition-Aerobic,
days- 4-7

14. Azotobactor bacteria
identiffication by gram staining
method

15. Bacillus megatherium bacteria
identification by gram staining
method

16. Azotobactor bacteria identiffication
by congo red indicator

17. Bacillus megatherium bacteria
identification by congo red
indicator

18. Result
 The study of PROM (phosphate rich organic
manure)producing bacteria’s name Azotobactor or
Bacillus Megatherium is present in the PROM
fertilizer we identified those bacteria’s by congo red
and gram staining method.