vp40- an ebloa virus matrix protein

1. VP 40
‘An Ebola virus protein’

2. Why was VP40 discovered
• Ebola outbreak in 2013
• 11,310 death
• 40% case fatality rate
• Research for antidote – study of virology
& transmission
• VP40 discovered
🧐
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fwww.telegraph.co.uk%2Fglobal-health

3. What is it?
• Viral matrix protein
🧐
• Source • Location
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fwww.sunnewsonline.comhttps://www.google.co.in/url?sa=i&url=https%3A%2F%2Fabcnews.go.com

4. • Role
• Role
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fwww.arup.com
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fwww.shutterstock.com

5. Structure
 Monomeric protein.
 Matrix protein.
 Made up of two domains :
a) N - terminal domain
b) C - terminal domain
 Hexamer of VP40 is essential
https://www.google.com/url?sa=i&source=imgres

6. https://www.google.com/url?sa=i&source=imgres&cd=&cad=rja&uact=8&ved
https://www.google.com/url?sa=i&source=imgres&cd=&cad=rja&uact=8&ved

7. What does it do?
• VP40 is a critical target for novel antifiloviral therapeutic agent
development
• VP40 exists in multiple conformational states during EBOV infection
• VP40 interacts directly with the plasma membrane to promote virion
assembly
• VP40 recruits host Endosomal Sorting Complex Required for Transport
family members to mediate efficient virion assembly

8. Zaire ebolavirus
• 7 genes – 10 proteins
• Proteins – NP,VP,GP
• Viral entry – GP1,GP2
• Transcription & replication:
 RNP complex formation
 RNP release
 cRNA formation
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fviralzone.expasy.org

9. Zaire ebolavirus
• Assembly & budding:
 Proteins – GP,NP,VP40
https://www.google.co.in/url?sa=i&url=https%3A%2F%2Fmicrobiologyinfo.com

10. Fasta sequence
https://www.ncbi.nlm.nih.gov/protein/AKI82619.1?report=fasta

11. Why is it important?
• High Mortality rate
• HHS Select Agent
• CDC Category A Bioterrorism Agent
• Biological Agent for Export Control by Australian Group
• Sufficient to generate virus like particles

12. Thank you