This project is been done to evaluate the tolerence level of the different trees species to air pollution w.r.t 4 different parameters i.e Total Chlorophyll (TCh), pH of the leaf extract, Relative Water Content (RWC) & AIR POLLUTION TOLRENCE INDEX (APTI) and further more.
Presentation: Farmer-led climate adaptation - Project launch and overview by ...
AIR POLLUTION TOLRENCE INDEX. (APTI)
1. Study of Air Pollution Tolerance Index of some plants from
Bhandup – Mulund Belt, Mumbai
Name of Participants:
Mohammed Owais ( S.Y.B.Sc student)
&
Sunita Chahar (Associate Professor)
International Virtual Conference on Life Science Research and its Interface
with Allied Sciences – ICLSRIAS-2022
January 11-13, 2022
2. WHY APTI ?
*To know the pollution tolerance level of the
trees.*
22-23
-Dec-2021
ICMRSTH-2021:
AS-SIRT,
SAGE
University,
Indore
2
3. Definition: The AIR POLLUTION TOLERENCE INDEX (APTI) is an
empirical relation which evaluates the tolerance level of plant
species toward air pollution from leaf biochemical parameters
such as:
• Leaf extract pH
• Relative Water Content (RWC)
• Ascorbic Acid
• Total Chlorophyll
APTI is used in studies like the Green belt development & pollution
studies.
*FORMULA: APTI= A(T+P) + R / 10
Where, A= Ascorbic acid content of the leaves
T= Total chlorophyll content of the leaves
P= pH of leaf extract
R= Relative Water Content of leaf (%)
4. Road side trees selected:
1. Ficus maclellandii
2. Polyalthia longifolia
3. Ficus religiosa
4. Thespesia populena
5. Alstonia scholanis
6. Annina squamosa
7. Ficus bengalhensis
8. Psidium gujava
9. Trema orientalis
10. Plumeria alba
]
9. TREES SELECTED :
9. Trema orientalis 10. Plumeria alba
22-23
-Dec-2021
ICMRSTH-2021:
AS-SIRT,
SAGE
University,
Indore
9
10. PROCEDURE / STEPS :
10 Different variety of tree leaves were collected from 10
different sites.
Selected 3 leaves from each tree for ‘fresh weight’
category.
Then soaked all the selected3 leaves overnight in a beaker.
Weighed all the soaked selected leaves and recorded the
new weight in the ‘turgid weight’ category.
Kept the selected leaves for drying for ‘dry weight‘
category.
Then selected some leaves for the pH extraction.
22-23
-Dec-2021
ICMRSTH-2021:
AS-SIRT,
SAGE
University,
Indore
10
11. CONTD :
After drying the selected leaves overnight, they were kept in oven
at 85-90.
There were process like the titration, centrifugation to know the
wave lengths.
After recording all the weight of 3 categories they were further
combined and their mean value was calculated as the ‘final weight’.
After crisp drying the selected leaves, they were measured again
and taken weight for the ‘dried’ category.
The other readings which were taken is, (DCPIP, pH, final weight,
Tch, RWC & APTI).
12. What is DCPIP ?
2,6-dichlorophenolindophenol, ( DCPIP ) is a blue chemical compound
used as a redox dye. Oxidized DCPIP is blue, reduced DCPIP is colorless.
This reaction is reversible, as colorless DCPIP can be reoxidized to blue.
DCPIP can be used to measure the rate of photosynthesis.
13. What’s RWC ?
Relative Water Content is described as the amount of
water in a leaf at the time of sampling relative to the
maximal water a leaf can hold.
It is an important parameter in water relation studies,
e.g. it allows the calculation of the osmotic potential at
full turgid.
14. What’s APTI ?
Air pollution tolerance index (APTI) reflects a plant's
ability to tolerate air pollution. Plants with higher APTI
can act as a source of pollution alleviation, whereas
plants with lower APTI can be an indicator of air
pollution level.
15. How will one prepare
DCPIP solution?
Indophenol (DCPIP) solution: Dissolve approximately
0.04 g of sodium hydrogen carbonate in 200 mL of
distilled water, add approximately 0.05 g DCPIP and
dissolve with shaking. This is very hard to dissolve. Leave
overnight and filter.
