cellulose to bioethanol

1. CELLULOSE ISOLATION AND
FERMENTATION OF LEAF WASTE
(Pterocarpus indicus) FOR BIOETHANOL
PRODUCTION
By:
1. Reyja Akbar Kertadiwangsa
2. Aussie Rezali Ramadhan Bawolje
Junior
3. Arviandra Haryo Nariswara
4. Mochammad Alief Rahman

2. Background
1. Sustainable Development Goals (SDGs) are global action plans agreed by world leaders,
including Indonesia, in protecting the environment. SDGs 2030 number 7 relates to clean
and affordable energy, (Kumar et al,. 2016)
2. Surabaya is one of the cities that has a lot of green open space, 573 city parks, with a
total park area of around 1,651 hectares. The number of trees in Surabaya can potentially
lead to the accumulation of leaf litter. (A Budiman et al,. 2014)
3. Bioethanol can be made from cellulose through fermentation using microorganisms
(Riyanti et al,. 2009)
INTRODUCTION

3. METHOD AND EXPERIMENTAL DETAILS
TYPE OF RESEARCH
Experimental research conducted using both quantitative
and qualitative data analysis
PLACE AND TIME
RESEARCH TARGET
Bioethanol from fermentation of cellulose isolation of
Pterocarpus indicus
RESEARCH STAGES
The research was conducted at the Biology Laboratory
SMAN 16 Surabaya (Fermentation and Isolation of
Cellulose) and State University Chemistry Lab, Surabaya
(FTIR Test) in June - September 2022
- Cellulose Isolation
- Cellulose Fermentation
- Organoleptic and FTIR Test

4. DISCUSSION
Cellulose Isolation and Fermentation
Prehydrolysis
Delignification
Bleaching II
Fermentation
Bleaching I
90

5. Angsana
leaves Powder
before
experiment
RESULTS
Step
Picture
Color
Texture
Pretreatment Prehydrolysis
Delignificatio
n
Bleaching I Bleaching II
Fermentatio
n
Residue after
prehydrolysis
Residue after
Delignification
Residue after
1st bleaching
Residue after
2nd bleaching
Fermented
residue
Brown Pickle Green Orange
Greyish
White
White Grey
Rough,
powder-like
Rough, mushy,
moist
Rough, moist,
compact
Mushy, moist Mushy, moist Mushy

6. Cellulose FTIR Test Results

7. Wavenumber (cm-1)
Range Functional Group
Interpretation
3338,17 3400-3200 O-H Stretching
Vibration
2902,04 3000-2850 Sp3 C-H Stretching
1740-1720 C=O Stretching
1639,16 1650-1630 OH (water)
1600-1500 C=C aromatic aromatic
ring
1368,98 1440-1000 C-O-H Bending
895,47 Around 850 Asymetric C-O-C
Stretching Vibration

8. No. Angsana Leaf Powder Yield Ethanol Concentration
1. 90 gram 10 gram 48%

9. POTENTIAL
BIOETHANOL
It is renewable and reduces
GHG emissions, by more
than 71% compared to a
fossil fuel.
Reduces other pollutants
emissions, improving air
quality.
It is biodegradable and has
low toxicity.

10. CONCLUSION
Based on the research that has been done, fermentation of Pterocarpus indicus
leaves can be processed into bioethanol by certain procedures, are cellulose
isolation and cellulose fermentation. Cellulose isolation was carried out through
the stages of prehydrolysis, delignification, and bleaching. The isolated cellulose
was then fermented using Saccharomyces cerevisiae. Quantitative analysis
showed that the yield of cellulose produced was 10 grams. Cellulose
fermentation FTIR test results showed the presence of 48% bioethanol content.
Qualitative analysis was performed by organoleptic test and FTIR (Fourier
Transform Infrared Spectroscopy) test. The result of the organoleptic test
showed that the cellulose produced was white. While the results of cellulose
fermentation showed a porridge-like texture.

11. RESEARCH DOCUMENTATION

12. THANK YOU