Laura Uribe García - 3 semestre UPB

1. Laura Uribe García
Universidad Pontificia Bolivariana, Facultad de Medicina
Biología Molecular, III semestre

2. Kaposi's sarcoma (KS)
Type of cancer formed in the lining of blood and lymph
vessels.
The cause of Kaposi’s sarcoma is an infection with the
human herpesvirus 8 (HHV-8).
KS is the most common AIDS-associated tumor.
The lesions of Kaposi’s sarcoma appear as painless
purplish spots on legs, feet, face, mouth, genital area,
digestive tract and lungs.
AIDS: chronic condition caused by the human
immunodeficiency virus (HIV), which damage the
immune system by interfering with the body’s ability
to fight the organisms that cause disease.
There’s no cure for HIV/AIDS.
The symptoms of HIV and AIDS vary, depending on
the phase of infection.
Kaposi's sarcoma and Lymphoma are common
cancer’s to HIV/AIDS
HIV/AIDS

3. Staphylococcus aureus
Is the most dangerous and the one who causes most of
the infections by staphylococcus.
It usually causes infection at the skin, bones, sepsis,
pneumonia and endocarditis.
Staphylococcus can spread from one person to another.
They can also be found in objects.
Its associated with periodontitis, more commonly in
patients with HIV-positive.
Herpesvirus (HV) causes a great number of
infections, in the orofacial and genital area
HHV-8 is the responsible of Kaposi’s sarcoma on
HIV-positive patients, because of the
immunosuppression. When it is activated, a
malignant tumor is developed.
Herpesvirus

4. Objective
Understand whether and how S.
aureus and its components affect
KSHV lytic reactivation from oral
cells and the clinical prevalence and
relevance of their coinfection in oral
cavities of HIV-positive patients.

5. ELISAs (quantitative enzyme-linked immunosorbent assay)
it's a serologic technique designed for
detecting and quantifying substances
such as peptides, proteins, antibodies
and hormones by using an antigen or
an antibody absorbed on a solid phase.
It was used on the determination of the
KSHV infection status.

6. RT-PCR (quantitative Reverse Transcription-Polymerase Chain Reaction)
It is the RNA amplification through its
cDNA synthesized a priori, using a
reverse transcriptase, followed by several
cycles of conventional PCR.
It was used to detect bacteria's an viruses
(as S. aureus and KSHV) on saliva
samples.

7. Immunoblot
it's based on the reaction of an antibody
with an antigen. When they react, the
proteins are separated and are recognized
with specific antibodies.
The expression of proteins (TLR2, TLR4,
siRNA) was detected by using this method.

8. Results

12. Discussion
AUTHOR CONCEPT ?
[39] Weinke T, Schiller R,
Fehrenbach FJ, Pohle HD
“S. aureus has been shown to colonize the anterior nares
of HIV-positive patients with greater frequency than in the
general population”
[48] Dai L, Del Valle L, Miley W, et al.
“We found that KSHV infection greatly increased S.
aureus internalization at a very early time point after
bacterial infection.”
[50] Antman K, Chang Y
“Cutaneous and mucosal KS has a typical progression,
beginning with an early patch and progressing to an
intermediate plaque, followed by formation of tumor
nodules”

13. Conclusion
1. HIV, because of immune suppression,
makes HIV-positive and AIDS patients
more susceptible to some pathogen's
agents (as HHV8), than health people,
with a normal immune systmen function.
2. PCR let us know how is the prevalence
of KSHV and S.aureus infections on HIV
positive patients.