2. CONTENTS
INTRODUCTION.
TYPES OF PAPER CHROMATOGRAPHY.
1. Ascending chromatography.
2. Descending chromatography.
3. Ascending-descending chromatography.
4. Radial chromatography.
5. Two dimensional chromatography.
APPLICATIONS OF PAPER CHROMATOGRAPHY.
CONCLUSION.
REFERENCES.
3. INTRODUCTION
Dr.M.Tswett introduced paper chromatography in
1906.
Paper chromatography is simple and most widely
used separation technique.
Partition chromatography principle.
The separation is effected by differential migration
of the mixture of substances, which takes place due
to difference in partition.
Analysis of unknown substances.
4. TYPES OF PAPER CHROMATOGRAPHY
1. Ascending Chromatography.
2. Descending Chromatography.
3. Ascending-Descending Chromatography.
4. Radial or Circular Chromatography.
5.Two dimensional Chromatography.
10. APPLICATIONS OF PAPER CROMATOGRAPHY
A qualitative method for identifying components
of mixture.
Identification of drug and impurities
In forensic studies( DNA and RNA sequencing).
As an analytical chemistry technique (Colored
mixtures like pigments).
Unknown organic and inorganic compounds.
Identification of biomolecules .
It’s an inexpensive and rapid method for
separation.
11. CONCLUSION
From this presentation, I came to know about
importance and types of paper chromatography.
How it is useful in separation and analysis of
mixture of components.
Paper chromatography is widely used in areas
pharmaceutical and many chemical industries.
As it is inexpensive and simple, it is basic
chromatographic technique performed in
laboratories.
12. REFERENCES
Gurdeep R Chatwal and Sham K Anand.2013.”Instrumental
methods of chemical analysis”,1st Edition , Himalaya
Publishing House. :2.590-2.592
R.P. BUDHIRAJA. Copy right 2OO4”Separation Chemistry”,
New Age International (P) Ltd.
240-246
http://namrataheda.blogspot.in/search/label/types%20of%20paper%20chro
matography.
www.tutorvista.com/biology/uses-of-paper-chromatogarphy?view=simple