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76
Journal of Global Resources Volume 6 (01) August 2019-January 2020 Page 76-80
UGCโ€“CARE Listed Journal in Group D ISSN: 2395-3160 (Print), 2455-2445 (Online)
12
NUTRITIVE VALUE OF DIFFERENT PARTS OF FIVE TYPES OF SOLENOSTEMON
SCUTELLARIOIDES (L.) CODD. FROM LAMIACEAE FAMILY
J. Chetia and L.R. Saikia
Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam, India
Email: junali.chetia@yahoo.com
Abstract: Solenostemon scutellarioides (L.) Codd. from Lamiaceae family are ornamental
plants having medicinal value. As some of the Lamiaceae members (Mentha spp, Ocimum
spp.) are used as vegetable food, the nutritional status of these ornamental plant samples were
determined to gets an idea about their food value using standard laboratory methods. All the
samples are found to have nutritional value above 300cal/kg. Young leaves of type 5 recorded
highest nutritive values (505.55cal/kg) than other tested types of S. scutellarioides. The ash,
moisture, fat, protein and carbohydrate contents vary from type to type of S. scutellarioides.
Perhaps all the selected plants are not equally used as food in spite of their importance as
medicinal resource.
Key words: Solenostemon scutellarioides (L.) Codd., Lamiaceae members
Introduction
Lamiaceae has occupied an important position in the socio-cultural, spiritual, medicinal aspects
of rural and tribal peoples of India with their various medicinal properties (Sankar et al., 1994;
Singh and Majumdar, 1997; Umadevi and Ganasoundari, 1999; Prakash and Gupta, 2000;
Mediratta et al., 2002). The fat, ash, moisture, carbohydrate, protein content and nutritive value
of Lamiaceae members are also described by some workers (Edeoga et al., 2006; Kavitha et
al., 2009; Idris et al., 2011; Khomdram et al., 2011; Koche et al., 2011; Mlitan et al., 2014;
Tomescu et al., 2015).
Solenostemon scutellariodes (L.) Codd. is an ornamental aromatic perennial plant
(Garcia and Oโ€™Neil, 2000; Prajapati et al., 2013) with various colours (De Loureiro, 1970). The
plants are rich in phenolic compound and rosmarinic acid (Rasineni et al., 2008; Razzaque and
Ellis, 1977; De-Eknamkul and Ellis, 1987; Petersen, 1991; Petersen and Simmonds, 2003;
Bauer et al., 2004). It is a folkloric medicinal plant used to treat malarial fever, hepatopathy,
renal and vesicle calculi, cough, chronic asthma, hiccough, bronchitis, helminthiasis, colic,
convulsions, diarrhoea, epilepsy, arthritic inflammations (Kirtikar and Basu, 1975; Warrier et al.,
1995). Some of the plants of Lamiaceae are used as vegetables and therefore, proximate
analysis of the samples were done to have an idea about the nutritional status of these plants
used as human food in addition to their medicinal importance.
ANALYSIS
Materials and Methods
Samples were collected from Dibrugarh district of Assam at their full bloomed stage. Different
parts (young and mature leaves, inflorescence and stem) were separated and cleaned
properly. The materials were air dried at room temperature. The stems were sliced before
allowed to dry. After removal of surface water, the materials were wrapped with brown paper
and allow sundry for complete dryness (less than 1-2 percent moisture content). The materials
were grounded to fine powder using mortar and pestle and then in electric grinder. The fine
powder was kept in air tight bottles for further analysis.
77
Photographs of the five types of Solenostemon scutellariodes (L.) Codd (a-e) (a-type 1;
b-type2, c-type 3, d-type 4 and e-type 5)
(a) (b) (c)
(d) (e)
Determination of Moisture Content
Moisture content was determined by the method described by AOAC, 1990. 3 gm of powdered
sample was weighed in flat bottom disc and kept for 24 hrs in a hot air over at + 80ยฐC and
finally weighed. The loss weight was regarded as a measure of moisture content.
Percent of Moisture =
๐‘Š๐‘’๐‘ก. ๐‘ค๐‘ก.โˆ’๐ท๐‘Ÿ๐‘ฆ ๐‘Š๐‘ก.
๐‘Š๐‘’๐‘ก ๐‘Š๐‘ก.
ร— 100
Determination of Ash Content
Ash content was determined by the method described by AOAC, 1990. 5 gm of powdered
sample was weighed in oven dried silica crucible. The crucible was heated first over a low
flame till the material completely charred, followed by heating in a muffle furnace for 3 hours at
3000
C. It was cooled in desiccator and weighted. To ensure completion of ashing, it was
heated again in the furnace for half an hour, cooled and weight. This was repeated
consequently till the weight become constant wt.
Percent of Ash =
๐‘Š๐‘ก.๐‘œ๐‘“๐ด๐‘ โ„Ž
๐‘Š๐‘ก. ๐‘œ๐‘“ ๐‘†๐‘Ž๐‘š๐‘๐‘™๐‘’
ร— 100
Determination of Fat Content
Fat content was determined by the method described by AOAC, (1990). 5 gm of moisture free
powdered sample was extracted with petroleum ether in a soxhlet extractor, heating the flask
for about 6 hrs till a drop taken from dripping left no greasy stain on filter paper. After boiling
with petroleum ether, the residual petroleum ether was filtered using whatman no. 40 filter
paper and filtrate was evaporated in a pre-weighed beaker. Increase in weight of beaker gave
crude fat.
Percent of fat =
๐‘Š๐‘’๐‘–๐‘”โ„Ž๐‘ก๐‘œ๐‘“๐‘กโ„Ž๐‘’๐น๐‘Ž๐‘ก
๐‘Š๐‘’๐‘–๐‘”โ„Ž๐‘ก๐‘œ๐‘“๐‘กโ„Ž๐‘’๐‘†๐‘Ž๐‘š๐‘๐‘™๐‘’
ร— 100
Determination of protein content
Protein content was determined by the Lowry method (Lowry et al., 1951)
Determination of carbohydrate content
Carbohydrate content was determined by using the formula as described by Indrayan et al.,
(2005)
78
Percent of carbohydrate = 100 โ€“ (% of ash + % of Moisture + % of fat + % of Protein)
Determination of nutritive value
The nutritive value of the plant parts were determined by the method described by Indrayan et
al., (2005).
Nutritive value = 4x percentage of protein + 9x Percentage of fat + 4x Percentage of
carbohydrate.
Nutritive value was expressed in Cal/ Kg of powder
Results
Ash content (percent) was recorded highest (5.70ยฑ0.50 percent) in mature leaves of type 1.
Moisture content (percent) was recorded highest (14.67ยฑ0.29 percent) in stem of type1. Fat
content (percent) was recorded higher (34.00ยฑ0.04%) in young leaves of type 5. Protein
content of different parts of five types ranges from 0.010 percent to 0.080 percent. Young
leaves of type 1 recorded highest protein content as 0.080 percent. Highest carbohydrate
content was recorded by stem of type 4 (92.84 percent). Kavitha et al., (2009) recorded
protein content as 61.4 to 90.5mg/g in 37 genotype of S. scutellariodes. All the samples are
found to have nutritional value above 300cal/kg. Perhaps all the selected plants are not equally
used as food in spite of their importance as medicinal resource. The study of Khomdram et al.
(2011) provides information on nutritive value of some selected plants of Lamiaceae collected
from Manipur. Carbohydrate, soluble amino acid and protein were recorded in variable
quantities in their samples. Perhaps these plants may contain some anti-nutritional factor as
reported by Vasconcelos and Oliveira (2004); Mattila et al., (2018). Gemede and Ratta, (2014)
explained that the presence of cyanogenic glycosides, protease inhibitors, lectins, tannins,
alkaloids and sapiens in the plants in higher quantities may cause anti-nutritional effect.
Table 01: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd.
Type1
Sample
โ†“
Ash
(%)
Moisture
(%)
Fat
(%)
Protein
(%)
Carbohydrate
(%)
Nutritive
value (cal/Kg)
Young leaf 1.81ยฑ0.03 14.60ยฑ1.09 2.60ยฑ2.00 0.080ยฑ0.01 80.91 347.36
Mature leaf 5.70ยฑ0.50 10.10ยฑ0.03 2.35ยฑ0.09 0.012ยฑ0.03 81.83 348.52
Inflorescence 3.00ยฑ0.22 10.00ยฑ0.00 1.67ยฑ0.46 0.034ยฑ0.11 83.84 350.53
Stem 1.09ยฑ0.04 14.67ยฑ0.29 0.87ยฑ0.01 0.012ยฑ0.90 83.35 341.27
Table 02:Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd.
Type 2
Sample
โ†“
Ash
(%)
Moisture
(%)
Fat
(%)
Protein
(%)
Carbohydrate
(%)
Nutritive
value
(cal/Kg)
Young leaf 1.43ยฑ0.01 9.00ยฑ0.27 2.90ยฑ0.00 0.012ยฑ0.22 86.65 372.77
Mature leaf 2.33ยฑ1.11 6.83ยฑ0.09 3.30ยฑ0.01 0.018ยฑ1.00 87.52 379.85
Inflorescence 1.70ยฑ0.99 12.23ยฑ1.09 2.00ยฑ0.38 0.023ยฑ0.98 74.04 354.27
Stem 0.98ยฑ0.01 12.78ยฑ1.12 0.89ยฑ0.02 0.013ยฑ0.22 85.33 349.41
79
Table 03: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd.
Type 3
Sample
โ†“
Ash
(%)
Moisture
(%)
Fat
(%)
Protein
(%)
Carbohydrate
(%)
Nutritive
value
(cal/Kg)
Young leaf 2.84ยฑ0.00 6.60ยฑ0.02 4.60ยฑ0.02 0.013ยฑ0.02 85.95 385.23
Mature leaf 1.96ยฑ0.07 7.20ยฑ0.33 5.81ยฑ1.00 0.014ยฑ0.11 85.02 392.42
Inflorescence 1.75ยฑ1.00 8.07ยฑ0.19 2.48ยฑ0.11 0.016ยฑ0.09 87.69 373.12
Stem 0.40ยฑ0.01 8.72ยฑ0.23 1.00ยฑ0.00 0.010ยฑ0.09 89.87 368.52
Table 04: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd.
Type 4
Sample
โ†“
Ash
(%)
Moisture
(%)
Fat
(%)
Protein (%)
Carbohydrate
(%)
Nutritive
value
(cal/Kg)
Young leaf 3.01ยฑ1.00 12.75ยฑ0.03 3.89ยฑ1.11 0.029ยฑ0.03 80.32 356.42
Mature leaf 2.00ยฑ0.44 14.10ยฑ1.00 4.00ยฑ0.03 0.030ยฑ0.32 79.87 355.60
Inflorescence 1.89ยฑ0.00 10.01ยฑ0.03 2.01ยฑ0.99 0.031ยฑ0.09 86.05 362.44
Stem 0.56ยฑ0.01 5.50ยฑ0.01 1.09ยฑ0.20 0.014ยฑ1.00 92.84 381.21
Table 05: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd.
Type 5
Sample
โ†“
Ash
(%)
Moisture
(%)
Fat
(%)
Protein
(%)
Carbohydrate
(%)
Nutritive
value
(cal/Kg)
Young leaf 2.36ยฑ0.04 13.75ยฑ2.01 34.00ยฑ0.04 0.018ยฑ0.01 49.87 505.55
Mature leaf 2.76ยฑ0.01 13.98ยฑ1.07 10.00ยฑ1.01 0.021ยฑ0.02 73.24 383.04
Inflorescence 2.09ยฑ1.11 10.40ยฑ0.22 8.57ยฑ0.99 0.035ยฑ0.77 78.91 392.89
Stem 1.00ยฑ0.03 6.50ยฑ0.01 3.12ยฑ0.09 0.012ยฑ0.09 89.36 385.59
Conclusion
This kind of study provides information about the nutritional quality of these plants and some of
them can be used as supplementary source of human food. The plants may have nutritional
value but they are still not considered as โ€˜functional foodโ€™ and are not well experimented.
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12. j. chetia and l.r. saikia

  • 1. 76 Journal of Global Resources Volume 6 (01) August 2019-January 2020 Page 76-80 UGCโ€“CARE Listed Journal in Group D ISSN: 2395-3160 (Print), 2455-2445 (Online) 12 NUTRITIVE VALUE OF DIFFERENT PARTS OF FIVE TYPES OF SOLENOSTEMON SCUTELLARIOIDES (L.) CODD. FROM LAMIACEAE FAMILY J. Chetia and L.R. Saikia Department of Life Sciences, Dibrugarh University, Dibrugarh, Assam, India Email: junali.chetia@yahoo.com Abstract: Solenostemon scutellarioides (L.) Codd. from Lamiaceae family are ornamental plants having medicinal value. As some of the Lamiaceae members (Mentha spp, Ocimum spp.) are used as vegetable food, the nutritional status of these ornamental plant samples were determined to gets an idea about their food value using standard laboratory methods. All the samples are found to have nutritional value above 300cal/kg. Young leaves of type 5 recorded highest nutritive values (505.55cal/kg) than other tested types of S. scutellarioides. The ash, moisture, fat, protein and carbohydrate contents vary from type to type of S. scutellarioides. Perhaps all the selected plants are not equally used as food in spite of their importance as medicinal resource. Key words: Solenostemon scutellarioides (L.) Codd., Lamiaceae members Introduction Lamiaceae has occupied an important position in the socio-cultural, spiritual, medicinal aspects of rural and tribal peoples of India with their various medicinal properties (Sankar et al., 1994; Singh and Majumdar, 1997; Umadevi and Ganasoundari, 1999; Prakash and Gupta, 2000; Mediratta et al., 2002). The fat, ash, moisture, carbohydrate, protein content and nutritive value of Lamiaceae members are also described by some workers (Edeoga et al., 2006; Kavitha et al., 2009; Idris et al., 2011; Khomdram et al., 2011; Koche et al., 2011; Mlitan et al., 2014; Tomescu et al., 2015). Solenostemon scutellariodes (L.) Codd. is an ornamental aromatic perennial plant (Garcia and Oโ€™Neil, 2000; Prajapati et al., 2013) with various colours (De Loureiro, 1970). The plants are rich in phenolic compound and rosmarinic acid (Rasineni et al., 2008; Razzaque and Ellis, 1977; De-Eknamkul and Ellis, 1987; Petersen, 1991; Petersen and Simmonds, 2003; Bauer et al., 2004). It is a folkloric medicinal plant used to treat malarial fever, hepatopathy, renal and vesicle calculi, cough, chronic asthma, hiccough, bronchitis, helminthiasis, colic, convulsions, diarrhoea, epilepsy, arthritic inflammations (Kirtikar and Basu, 1975; Warrier et al., 1995). Some of the plants of Lamiaceae are used as vegetables and therefore, proximate analysis of the samples were done to have an idea about the nutritional status of these plants used as human food in addition to their medicinal importance. ANALYSIS Materials and Methods Samples were collected from Dibrugarh district of Assam at their full bloomed stage. Different parts (young and mature leaves, inflorescence and stem) were separated and cleaned properly. The materials were air dried at room temperature. The stems were sliced before allowed to dry. After removal of surface water, the materials were wrapped with brown paper and allow sundry for complete dryness (less than 1-2 percent moisture content). The materials were grounded to fine powder using mortar and pestle and then in electric grinder. The fine powder was kept in air tight bottles for further analysis.
  • 2. 77 Photographs of the five types of Solenostemon scutellariodes (L.) Codd (a-e) (a-type 1; b-type2, c-type 3, d-type 4 and e-type 5) (a) (b) (c) (d) (e) Determination of Moisture Content Moisture content was determined by the method described by AOAC, 1990. 3 gm of powdered sample was weighed in flat bottom disc and kept for 24 hrs in a hot air over at + 80ยฐC and finally weighed. The loss weight was regarded as a measure of moisture content. Percent of Moisture = ๐‘Š๐‘’๐‘ก. ๐‘ค๐‘ก.โˆ’๐ท๐‘Ÿ๐‘ฆ ๐‘Š๐‘ก. ๐‘Š๐‘’๐‘ก ๐‘Š๐‘ก. ร— 100 Determination of Ash Content Ash content was determined by the method described by AOAC, 1990. 5 gm of powdered sample was weighed in oven dried silica crucible. The crucible was heated first over a low flame till the material completely charred, followed by heating in a muffle furnace for 3 hours at 3000 C. It was cooled in desiccator and weighted. To ensure completion of ashing, it was heated again in the furnace for half an hour, cooled and weight. This was repeated consequently till the weight become constant wt. Percent of Ash = ๐‘Š๐‘ก.๐‘œ๐‘“๐ด๐‘ โ„Ž ๐‘Š๐‘ก. ๐‘œ๐‘“ ๐‘†๐‘Ž๐‘š๐‘๐‘™๐‘’ ร— 100 Determination of Fat Content Fat content was determined by the method described by AOAC, (1990). 5 gm of moisture free powdered sample was extracted with petroleum ether in a soxhlet extractor, heating the flask for about 6 hrs till a drop taken from dripping left no greasy stain on filter paper. After boiling with petroleum ether, the residual petroleum ether was filtered using whatman no. 40 filter paper and filtrate was evaporated in a pre-weighed beaker. Increase in weight of beaker gave crude fat. Percent of fat = ๐‘Š๐‘’๐‘–๐‘”โ„Ž๐‘ก๐‘œ๐‘“๐‘กโ„Ž๐‘’๐น๐‘Ž๐‘ก ๐‘Š๐‘’๐‘–๐‘”โ„Ž๐‘ก๐‘œ๐‘“๐‘กโ„Ž๐‘’๐‘†๐‘Ž๐‘š๐‘๐‘™๐‘’ ร— 100 Determination of protein content Protein content was determined by the Lowry method (Lowry et al., 1951) Determination of carbohydrate content Carbohydrate content was determined by using the formula as described by Indrayan et al., (2005)
  • 3. 78 Percent of carbohydrate = 100 โ€“ (% of ash + % of Moisture + % of fat + % of Protein) Determination of nutritive value The nutritive value of the plant parts were determined by the method described by Indrayan et al., (2005). Nutritive value = 4x percentage of protein + 9x Percentage of fat + 4x Percentage of carbohydrate. Nutritive value was expressed in Cal/ Kg of powder Results Ash content (percent) was recorded highest (5.70ยฑ0.50 percent) in mature leaves of type 1. Moisture content (percent) was recorded highest (14.67ยฑ0.29 percent) in stem of type1. Fat content (percent) was recorded higher (34.00ยฑ0.04%) in young leaves of type 5. Protein content of different parts of five types ranges from 0.010 percent to 0.080 percent. Young leaves of type 1 recorded highest protein content as 0.080 percent. Highest carbohydrate content was recorded by stem of type 4 (92.84 percent). Kavitha et al., (2009) recorded protein content as 61.4 to 90.5mg/g in 37 genotype of S. scutellariodes. All the samples are found to have nutritional value above 300cal/kg. Perhaps all the selected plants are not equally used as food in spite of their importance as medicinal resource. The study of Khomdram et al. (2011) provides information on nutritive value of some selected plants of Lamiaceae collected from Manipur. Carbohydrate, soluble amino acid and protein were recorded in variable quantities in their samples. Perhaps these plants may contain some anti-nutritional factor as reported by Vasconcelos and Oliveira (2004); Mattila et al., (2018). Gemede and Ratta, (2014) explained that the presence of cyanogenic glycosides, protease inhibitors, lectins, tannins, alkaloids and sapiens in the plants in higher quantities may cause anti-nutritional effect. Table 01: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd. Type1 Sample โ†“ Ash (%) Moisture (%) Fat (%) Protein (%) Carbohydrate (%) Nutritive value (cal/Kg) Young leaf 1.81ยฑ0.03 14.60ยฑ1.09 2.60ยฑ2.00 0.080ยฑ0.01 80.91 347.36 Mature leaf 5.70ยฑ0.50 10.10ยฑ0.03 2.35ยฑ0.09 0.012ยฑ0.03 81.83 348.52 Inflorescence 3.00ยฑ0.22 10.00ยฑ0.00 1.67ยฑ0.46 0.034ยฑ0.11 83.84 350.53 Stem 1.09ยฑ0.04 14.67ยฑ0.29 0.87ยฑ0.01 0.012ยฑ0.90 83.35 341.27 Table 02:Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd. Type 2 Sample โ†“ Ash (%) Moisture (%) Fat (%) Protein (%) Carbohydrate (%) Nutritive value (cal/Kg) Young leaf 1.43ยฑ0.01 9.00ยฑ0.27 2.90ยฑ0.00 0.012ยฑ0.22 86.65 372.77 Mature leaf 2.33ยฑ1.11 6.83ยฑ0.09 3.30ยฑ0.01 0.018ยฑ1.00 87.52 379.85 Inflorescence 1.70ยฑ0.99 12.23ยฑ1.09 2.00ยฑ0.38 0.023ยฑ0.98 74.04 354.27 Stem 0.98ยฑ0.01 12.78ยฑ1.12 0.89ยฑ0.02 0.013ยฑ0.22 85.33 349.41
  • 4. 79 Table 03: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd. Type 3 Sample โ†“ Ash (%) Moisture (%) Fat (%) Protein (%) Carbohydrate (%) Nutritive value (cal/Kg) Young leaf 2.84ยฑ0.00 6.60ยฑ0.02 4.60ยฑ0.02 0.013ยฑ0.02 85.95 385.23 Mature leaf 1.96ยฑ0.07 7.20ยฑ0.33 5.81ยฑ1.00 0.014ยฑ0.11 85.02 392.42 Inflorescence 1.75ยฑ1.00 8.07ยฑ0.19 2.48ยฑ0.11 0.016ยฑ0.09 87.69 373.12 Stem 0.40ยฑ0.01 8.72ยฑ0.23 1.00ยฑ0.00 0.010ยฑ0.09 89.87 368.52 Table 04: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd. Type 4 Sample โ†“ Ash (%) Moisture (%) Fat (%) Protein (%) Carbohydrate (%) Nutritive value (cal/Kg) Young leaf 3.01ยฑ1.00 12.75ยฑ0.03 3.89ยฑ1.11 0.029ยฑ0.03 80.32 356.42 Mature leaf 2.00ยฑ0.44 14.10ยฑ1.00 4.00ยฑ0.03 0.030ยฑ0.32 79.87 355.60 Inflorescence 1.89ยฑ0.00 10.01ยฑ0.03 2.01ยฑ0.99 0.031ยฑ0.09 86.05 362.44 Stem 0.56ยฑ0.01 5.50ยฑ0.01 1.09ยฑ0.20 0.014ยฑ1.00 92.84 381.21 Table 05: Nutritive value of different parts of Solenostemon scutellarioides (L.) Codd. Type 5 Sample โ†“ Ash (%) Moisture (%) Fat (%) Protein (%) Carbohydrate (%) Nutritive value (cal/Kg) Young leaf 2.36ยฑ0.04 13.75ยฑ2.01 34.00ยฑ0.04 0.018ยฑ0.01 49.87 505.55 Mature leaf 2.76ยฑ0.01 13.98ยฑ1.07 10.00ยฑ1.01 0.021ยฑ0.02 73.24 383.04 Inflorescence 2.09ยฑ1.11 10.40ยฑ0.22 8.57ยฑ0.99 0.035ยฑ0.77 78.91 392.89 Stem 1.00ยฑ0.03 6.50ยฑ0.01 3.12ยฑ0.09 0.012ยฑ0.09 89.36 385.59 Conclusion This kind of study provides information about the nutritional quality of these plants and some of them can be used as supplementary source of human food. The plants may have nutritional value but they are still not considered as โ€˜functional foodโ€™ and are not well experimented. References 1. Association of Analytical Communities (AOAC) (1990) Official Methods of Analysis. 15th Edn, Association of Official Analytical Chemists, Washington, DC. USA., 200-210. 2. Bauer N, Leljak-Levanic D, Jelaska S (2004) Rosmarinic acid synthesis in transformed callus culture of Coleus blumei Benth. Zeitshrift fur Naturforschung. 59(7-8):554-60. 3. Edeoga HO, Omosun G, Uche LC (2006) Chemical composition of Hyptis suaveolens and Ocimum gratissimum hybrids from Nigeria. African Journal of Biotechnology. 5: 892-895. 4. De-Eknamkul W, Ellis BR (1987) Tyrosine aminotransferase: the entry point enzyme of the tyrosine-derived pathway in rosmarinic acid biosynthesis. Photochemistry. 26:1941-1946. 5. Garcia JF, Oโ€™Neil RJ (2000) Effect of Coleus size and variegation on attack rates, searching strategy, and selected life history characteristics of Cryptolaemus montrouzieri (Coleoptera: Coccinellidae). Biological Control. 18: 225-234. 6. Gemede HF, Ratta N (2014) Antinutritional factors in plant foods: Potential health benefits and adverse effects. International Journal of Nutritional and Food Sciences. 3(4): 284-289.
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