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Lab experience:
General Laboratory
 Pipette
 Weigh
 Sterilize
 Filtrate
 Titrate
 Wash/Clean glassware
 Prepare media
 Practice sterile techniques
Electron Microscopy
 Fix
 Stain
 Section
 Process film
 Print film
 Produce prints
 Operation of Electron Microscopy
 Prepare grids
 Form film on grids
Histology
 Cell and fiber stains
 Staining processes
Immunology
 Plaque assay for antibody forming cells
 Hemagglutination and hemolysis assay
 Florescent antibody technique
 Lyophilization
 Spectrofluorometry
 Isolate viruses
 Cytotoxicity assay
 Lymphocyte culture
Bacteriology
 Maintain and purify: bacteria, phage stocks, cell culture
 Tissue culture
 Culture transfers
 Pour plates
 Plasmid amplification/Prepare and assay bacteriophage lysates
Biochemistry/Genetics
 Purify and characterize enzymes
 Determinations: RNA, protein, nucleic acid, phosphate and others
 Gradients: density, equilibrium, etc.
 Assays: enzyme, infectivity, blood coagulation factors, and other
(biochemical) assays
 Electrophooresis: immune, acrylamyde gel, paper
 Column: ion exchange resins, gel, gas liquid, HPLC
 Analyze peptides
 Manipulate DNA
 Equilibrium sedimentation centrifugation
 Prepare anitibodies
 Analyze lipids
 Synthesize peptides
 Transduction
 Conjugation
Laboratory/Research Instruments
 Light microscope
 Microburett
 pH meter
 Centrifuges: standard, high speed
 Incubators
 Dark room equipment
 Precision balance
 Volumnetric glassware
 Oscilloscopes
 Microtomes: paraffin, celloidin
Other Skills or Relevant Experience
 Data processing
 Laboratory
 Statistical analysis
 Database skills

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Lab Experience

  • 1. Lab experience: General Laboratory  Pipette  Weigh  Sterilize  Filtrate  Titrate  Wash/Clean glassware  Prepare media  Practice sterile techniques Electron Microscopy  Fix  Stain  Section  Process film  Print film  Produce prints  Operation of Electron Microscopy  Prepare grids  Form film on grids Histology  Cell and fiber stains  Staining processes Immunology  Plaque assay for antibody forming cells  Hemagglutination and hemolysis assay  Florescent antibody technique  Lyophilization  Spectrofluorometry  Isolate viruses  Cytotoxicity assay  Lymphocyte culture Bacteriology  Maintain and purify: bacteria, phage stocks, cell culture
  • 2.  Tissue culture  Culture transfers  Pour plates  Plasmid amplification/Prepare and assay bacteriophage lysates Biochemistry/Genetics  Purify and characterize enzymes  Determinations: RNA, protein, nucleic acid, phosphate and others  Gradients: density, equilibrium, etc.  Assays: enzyme, infectivity, blood coagulation factors, and other (biochemical) assays  Electrophooresis: immune, acrylamyde gel, paper  Column: ion exchange resins, gel, gas liquid, HPLC  Analyze peptides  Manipulate DNA  Equilibrium sedimentation centrifugation  Prepare anitibodies  Analyze lipids  Synthesize peptides  Transduction  Conjugation Laboratory/Research Instruments  Light microscope  Microburett  pH meter  Centrifuges: standard, high speed  Incubators  Dark room equipment  Precision balance  Volumnetric glassware  Oscilloscopes  Microtomes: paraffin, celloidin Other Skills or Relevant Experience  Data processing  Laboratory  Statistical analysis