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TheRoleofTechnologyin Quantifying
MastitisRelatedDecisions
Dr. Jeffrey Bewley
Precision Dairy Monitoring
Second Green Revolution?
OR,TheWhiteRevolution?
Mastitis Detection isMostWanted
Most Useful Parameters Mean ± SD
Mastitis 4.77 ± 0.47
Standing heat 4.75 ± 0.55
Daily milk yield 4.72 ± 0.62
Cow activity 4.60 ± 0.83
Temperature 4.31 ± 1.04
Feeding behavior 4.30 ± 0.80
Milk components (e.g. fat, protein, and SCC) 4.28 ± 0.93
Lameness 4.25 ± 0.90
Rumination 4.08 ± 1.07
Hoof health 4.06 ± 0.89
1Results calculated by assigning the following values to response categories: Not
useful: 1, Of little usefulness: 2, Moderately useful: 3, Useful: 4, Very useful:5.
Matthew Borchers et al.
Shift Away from Treatment
Historical focus on treatment
Physical observations
Cow-side tests
Need more rapid and continuous measures
Recent proactive movement
Advancement in technologies
Mastitis Detection
Daily use of CMT and EC
Monthly testing of SCC
More recent focus
Introduction of in-line analyzers
Addition of EC to milk component data
Behavior
Early Disease Detection
Detect diseases
earlier than with
visual observation
alone
Improve individual
cow treatment
results
Indicate a larger,
herd-level, problem
leading to improved
prevention strategies
Mastitis Detection Benefits
Reduced labor
Early treatment and intervention
Cultured based therapy?
NSAID administration?
Pathogen-specific approach?
Mastitis Detection Benefits
Increase in bacteriological cure
Less chronic cases?
Increase in well-being
Fewer severe cases
Preemptive NSAID administration based on
culture results
Reduced chronic cases
Faster more appropriate treatment regimes
Mastitis Detection Benefits
Reduced mastitis transmission
Contagious animals separated sooner
Separate abnormal milk
Threshold in component changes
For example, presence of blood
Use of management protocols already in
place to address sick cows
Drying off
Abrupt cessation is US industry norm
Milk leakage and discomfort are concern
Increase risk of IMI with > 17.5 kg/d
Primiparous animals show reduced risk of IMI
with gradual cessation
Role in tailoring drying off approach
Selective dry cow therapy
(Gott et al., 2016)
(Rajala-Schultz et al., 2005)
Herd or group or shift monitoring
What Technologies are Out
There?
Electrical Conductivity
Ion concentration of milk changes, increasing
electrical conductivity
Inexpensive and simple equipment
Wide range of sensitivity and specificity
reported
Results improve with quarter level sensors
Improved results with recent algorithms
Most useful combined with other metrics
Milk Color
Color variation (red, blue, and green)
sensors in some automatic milking systems
Reddish color indicates blood (Ordolff,
2003)
Clinical mastitis may change color patterns
for three colors (red, green and blue)
Specificity may be limited
www.lely.com
Temperature
Not all cases of mastitis result in a
temperature response
Best location to collect temperature?
Noise from other physiological impacts
Thermography
May be limited because not all cases of mastitis
result in a temperature response
Difficulties in collecting images
Hovinen et al., 2008; Schutz, 2009
Before Infection After Infection
Inline Somatic Cell Count
CellSenseDeLaval OCC
Lely MQCCMastiline
< 200,000
200,000 — 400,000
400,000 — 800,000
800,000 — 2,000,000
> 2,000,000
SCC value
Works like a traffic light
Spectroscopy
Visible, near-infrared, mid-infrared, or radio
frequency
Indirect identification through changes in
milk composition
AfiLab uses near infrared
Fat, protein, lactose
BiosensorsandChemicalSensors
Biological components (enzymes, antibodies, or
microorganism)
Enzyme, L-Lactate dehydrogenase (LDH), is released
because of the immune response and changes in cellular
membrane chemistry
Chemical sensors: changes in chloride, potassium, and
sodium ions, volatile metabolites resulting from mastitis,
haptoglobin, and hemoglobin (Hogeveen, 2011)
Milk measurements
Progesterone
Heat detection
Pregnancy detection
LDH enzyme
Early mastitis detection
BHBA
Indicator of subclinical ketosis
Urea
Protein status
Wearable Technologies
NeckorEar BasedBehavior
Monitoring
On-farm evaluation of lying time:
Identification of cows requiring
attention (lameness, illness, estrus)
Assessment of facility
functionality/cow comfort
Assess animal well-being
LyingBehaviorMonitoring
Real TimeLocation Systems
Current Research Limitations
Examines changes retrospectively
Changes may be seen around
subclinical and clinical disease
Extrapolation? Is it appropriate?
How much?
Breed, housing type, region?
We find differences….but so what?
MastitisChallenges
Meeting sensitivity (80%) and specificity
(99%) goals (Rasmussen, 2004)
Calibration across time
Automatic diversion or alert?
Recommended action when an alert occurs
with no clinical signs
Mastitis Challenges
Dynamics of clinical and subclinical
mastitis
Potential for overtreatment
Employee education
Gold standards imperfect
Mastitis Challenges
No growth
Different pathogens behave
differently
Changes affected by
Heat stress, estrus, production, overall
health, calving, etc.
How good are
we at finding
events of
interest?
Reasonsalertswerenotchecked
36%
15%
2%1%
9%
8%
6%
5%
5%
2%
2%
3% 3% 3%
No flakes/clots on filter sock
Milk production not alarming
Repeat
No time
Combination alert not alarming
Temporary physical problems
Conductivity alert not alarming
AMS disorders
Too many cows treated
Green alert
Checked before, not clinical
Not clinical at last check
Will be culled
In heat
Hogeveen et al., Precision Livestock Farming ‘13
*Farmers only checked 3% of alerts and missed 74% of mastitis cases
HowManyCowsWithConditionDoWeFind?
Example: 100 mastitis events
80 Mastitis Events Identified by Technology
20 Mastitis Events
Missed by Technology
HowManyAlertsCoincidewithanActualEvent?
Example: 100 mastitis alerts
90 Alerts for Cows that Have Mastitis
10 Alerts for Cows that
Do No Have Mastitis
Are we focused too heavily
on disease detection?
Other Cautions
• Huge within cow and within herd variation
• Many management factors and
environmental conditions affect these
variables
• Group/pen changes affect behaviors
• Some cows don’t read the book
• Not all changes are linear
Where do we go from here?
Identify disease prospectively
Determine course of action after alert
Culture?
Treatment?
Another intervention?
Assess economic benefit of
identification
Questions?
Jeffrey Bewley, PhD, PAS
jbewley@bovisync.com
jbewley@cowfocused.com

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The Role of Technology in Quantifying Mastitis Related Decisions

  • 3.
  • 6. Mastitis Detection isMostWanted Most Useful Parameters Mean ± SD Mastitis 4.77 ± 0.47 Standing heat 4.75 ± 0.55 Daily milk yield 4.72 ± 0.62 Cow activity 4.60 ± 0.83 Temperature 4.31 ± 1.04 Feeding behavior 4.30 ± 0.80 Milk components (e.g. fat, protein, and SCC) 4.28 ± 0.93 Lameness 4.25 ± 0.90 Rumination 4.08 ± 1.07 Hoof health 4.06 ± 0.89 1Results calculated by assigning the following values to response categories: Not useful: 1, Of little usefulness: 2, Moderately useful: 3, Useful: 4, Very useful:5. Matthew Borchers et al.
  • 7. Shift Away from Treatment Historical focus on treatment Physical observations Cow-side tests Need more rapid and continuous measures Recent proactive movement Advancement in technologies
  • 8. Mastitis Detection Daily use of CMT and EC Monthly testing of SCC More recent focus Introduction of in-line analyzers Addition of EC to milk component data Behavior
  • 9. Early Disease Detection Detect diseases earlier than with visual observation alone Improve individual cow treatment results Indicate a larger, herd-level, problem leading to improved prevention strategies
  • 10. Mastitis Detection Benefits Reduced labor Early treatment and intervention Cultured based therapy? NSAID administration? Pathogen-specific approach?
  • 11. Mastitis Detection Benefits Increase in bacteriological cure Less chronic cases? Increase in well-being Fewer severe cases Preemptive NSAID administration based on culture results Reduced chronic cases Faster more appropriate treatment regimes
  • 12. Mastitis Detection Benefits Reduced mastitis transmission Contagious animals separated sooner Separate abnormal milk Threshold in component changes For example, presence of blood Use of management protocols already in place to address sick cows
  • 13. Drying off Abrupt cessation is US industry norm Milk leakage and discomfort are concern Increase risk of IMI with > 17.5 kg/d Primiparous animals show reduced risk of IMI with gradual cessation Role in tailoring drying off approach Selective dry cow therapy (Gott et al., 2016) (Rajala-Schultz et al., 2005)
  • 14. Herd or group or shift monitoring
  • 15. What Technologies are Out There?
  • 16. Electrical Conductivity Ion concentration of milk changes, increasing electrical conductivity Inexpensive and simple equipment Wide range of sensitivity and specificity reported Results improve with quarter level sensors Improved results with recent algorithms Most useful combined with other metrics
  • 17. Milk Color Color variation (red, blue, and green) sensors in some automatic milking systems Reddish color indicates blood (Ordolff, 2003) Clinical mastitis may change color patterns for three colors (red, green and blue) Specificity may be limited www.lely.com
  • 18. Temperature Not all cases of mastitis result in a temperature response Best location to collect temperature? Noise from other physiological impacts
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  • 20. Thermography May be limited because not all cases of mastitis result in a temperature response Difficulties in collecting images Hovinen et al., 2008; Schutz, 2009 Before Infection After Infection
  • 21. Inline Somatic Cell Count CellSenseDeLaval OCC Lely MQCCMastiline
  • 22. < 200,000 200,000 — 400,000 400,000 — 800,000 800,000 — 2,000,000 > 2,000,000 SCC value Works like a traffic light
  • 23. Spectroscopy Visible, near-infrared, mid-infrared, or radio frequency Indirect identification through changes in milk composition AfiLab uses near infrared Fat, protein, lactose
  • 24. BiosensorsandChemicalSensors Biological components (enzymes, antibodies, or microorganism) Enzyme, L-Lactate dehydrogenase (LDH), is released because of the immune response and changes in cellular membrane chemistry Chemical sensors: changes in chloride, potassium, and sodium ions, volatile metabolites resulting from mastitis, haptoglobin, and hemoglobin (Hogeveen, 2011)
  • 25. Milk measurements Progesterone Heat detection Pregnancy detection LDH enzyme Early mastitis detection BHBA Indicator of subclinical ketosis Urea Protein status
  • 28. On-farm evaluation of lying time: Identification of cows requiring attention (lameness, illness, estrus) Assessment of facility functionality/cow comfort Assess animal well-being LyingBehaviorMonitoring
  • 30. Current Research Limitations Examines changes retrospectively Changes may be seen around subclinical and clinical disease Extrapolation? Is it appropriate? How much? Breed, housing type, region? We find differences….but so what?
  • 31. MastitisChallenges Meeting sensitivity (80%) and specificity (99%) goals (Rasmussen, 2004) Calibration across time Automatic diversion or alert? Recommended action when an alert occurs with no clinical signs
  • 32. Mastitis Challenges Dynamics of clinical and subclinical mastitis Potential for overtreatment Employee education Gold standards imperfect
  • 33. Mastitis Challenges No growth Different pathogens behave differently Changes affected by Heat stress, estrus, production, overall health, calving, etc.
  • 34. How good are we at finding events of interest?
  • 35. Reasonsalertswerenotchecked 36% 15% 2%1% 9% 8% 6% 5% 5% 2% 2% 3% 3% 3% No flakes/clots on filter sock Milk production not alarming Repeat No time Combination alert not alarming Temporary physical problems Conductivity alert not alarming AMS disorders Too many cows treated Green alert Checked before, not clinical Not clinical at last check Will be culled In heat Hogeveen et al., Precision Livestock Farming ‘13 *Farmers only checked 3% of alerts and missed 74% of mastitis cases
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  • 37. HowManyCowsWithConditionDoWeFind? Example: 100 mastitis events 80 Mastitis Events Identified by Technology 20 Mastitis Events Missed by Technology
  • 38. HowManyAlertsCoincidewithanActualEvent? Example: 100 mastitis alerts 90 Alerts for Cows that Have Mastitis 10 Alerts for Cows that Do No Have Mastitis
  • 39. Are we focused too heavily on disease detection?
  • 40. Other Cautions • Huge within cow and within herd variation • Many management factors and environmental conditions affect these variables • Group/pen changes affect behaviors • Some cows don’t read the book • Not all changes are linear
  • 41. Where do we go from here? Identify disease prospectively Determine course of action after alert Culture? Treatment? Another intervention? Assess economic benefit of identification
  • 42. Questions? Jeffrey Bewley, PhD, PAS jbewley@bovisync.com jbewley@cowfocused.com